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Studies on the Affinity Membranes for Adsorption and Purification of Lysozyme(Ⅱ) Adsorption Characteristics of Metal Chelating Affinity Membranes for Lysozyme

LI Jing, CHEN Huan Lin *, CHAI Hong (College of Chemical Engineering, Zhejiang University, Hangzhou 310027, China)  
Based on activation, coupling and chelating processes, PP(polypropylene) microporous membranes whose surface was treated by low temperature plasma were used for preparation of affinity membranes with Fe 3+ , Ni 2+ , Cu 2+ and Zn 2+ metal chelating ligand respectively. Those affinity membranes have been used for adsorption desorption experiments of lysozyme. The experimental results showed that adsorption capacity of the membranes with Ni 2+ or Cu 2+ metal chelating ligand is higher than that with Fe 3+ or Zn 2+ metal chelating ligand. The adsorption capacity of affinity membrane made in Cl - ion solution is also higher than that made in SO 2- 4 ion solution. The experimental results also showed that affinity membranes with Ni 2+ or Cu 2+ can be reused several times while affinity membrane with Fe 3+ ion can not. Adsorption amount of lysozyme can be partly restored by addition of metal ions on the reused affinity membranes.
【Fund】: 国家自然科学基金! (批准号 :2 96760 38)资助课题
【CateGory Index】: TQ465
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