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Analysis of Biologically Active Compounds in Chinese Medicines by Molecular Biochromatography with Human Serum Albumin(HSA) Stationary Phase

KONG Liang, ZOU Han-Fa* WANG Hai-Lin, NI Jian-Yi, ZHANG Yu-Kui (National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116011, China)  
A strategy for analysis of bioactive compounds in traditional Chinese medicine was first proposed by molecular biochromatography with human serum albumin(HSA) stationary phase. Separation of active compounds in four kinds of traditional Chinese medicine(TCM) was compared on the HSA column, and found that different TCM show different characteristic of chromatogram patterns. The effects of extracting solutions(water/methanol/ethanol) and extracted time on the amount of bioactive compounds in Angelica sinensis(Oliv.) Diels were examined, and a quality control method was also developed based on this technique, which is able to simultaneously determine the concentrations of ferulic acid and liguistilide in extracted solutions of Angelica sinensis(Oliv.) Diels by water and methanol.
【Fund】: 国家杰出青年基金! (批准号 :2 972 5512 )资助
【CateGory Index】: O657.7
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