Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《CHEMICAL RESEARCH IN CHINESE UNIVERSITIES》 2000-06
Add to Favorite Get Latest Update

Studies on Plant Extracellular Calmodulin by Lanthanide Luminescence Probe

LIU De Long 1,* , SUN Da Ye 2, YANG Yan Sheng 3 [WT5”BX](1. Department of Chemistry, 2. Department of Biology, Hebei Normal University, Shijiazhuang 050091, China; 3. Department of Chemistry, Zhongshan University, Guangzhou 510275, China) [WT  
Plant extracellular calmodulin(CaM) has been purified from cauliflower and identified with NAD kinase(NADK) activation and inhibition effect of CaM antagonist W7. Tb 3+ fluorescence titration showed that extracellular CaM contained four metal binding sites. The excitation spectrum and emission specturm indicated that extracellular CaM contained one tyrosine residue which could transfer energy to bound Tb 3+ . Based on Frster type nonradiative energy transfer theory, the distances of Tyr→sites Ⅲ, Ⅳ have been determined, these are 1 104 nm(Tyr→Ⅲ, site) and 1 056 nm(Tyr→Ⅳ, site). By studing the effect of CaM antagonist W7 and CaM antibody on Tb 3+ sensitized fluorescence, it was found that the binding sites of W7 and antibody were located on the c terminal part of plant extracellular CaM which contains domain Ⅲ and domain Ⅳ. [WT5HZ]
【Fund】: 国家自然科学基金!重点项目 (批准号 :3 973 0 2 3 0 );; 中国科学院长春应用化学研究所稀土化学与物理开放实验室资助
【CateGory Index】: O614
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 XU Xiao Long, LIU Qing Liang *, XIE Yong Shu (Department of Chemistry, University of Science and Technology of China, Hefei 230026, China);Studies on Anticoagulation Factor Ⅱ from Agkistrodon Acutus Venom by Rare Earth Ion Fluorescence Probes[J];Chemical Research In Chinese Universities;2001-11
2 LI Wei-Guo 1, QI Chao 1,2, DU Li 2, LIU Li-Yan 1, ZHAO Da-Qing 1* (1. Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; 2. Department of Biochemistry, Quartermaster University of PLA, Changchun 130062, China);Influence of Rare Earth Irons on the Molecular Recognition Between Calmodulin and Monoclonal Antibody[J];Chemical Research In Chinese Universities;2003-08
3 XIAO Feng juan 1, LIU De long 2, BAI Juan 3, SUN Da ye 3 1. Material Branch of College, College of Railway, Shijiazhuang 050043, China 2. Department of Chemistry, Hebei Normal University, Shijiazhuang 050091, China 3. College of Life Science, Hebei Normal University, Shijiazhuang 050091, China;Fluorescence of Tb~(3+)-Calmodulin Complex and Its Analytical Application[J];Spectroscopy and Spectral Analysis;2004-08
4 WANG Hui-juan,GUO Li-jian,YANG Ning (Hebei College of Industry and Technology,Shijiazhuang 050091, China);Advance in Studies on Calmodulin by Tb~(3+) Luminescence Probe[J];Hebei Chemical Engineering and Industry;2008-08
5 XIAO Feng_juan 1 ,CHANG Hong 2 ,LIUDe_long 3 (1.Department of Material Science and Engineering,Shijiazhuang Railway Institute,Shijiazhuang050043,China; 2.College of Physical Sciences,Hebei Normal University,Shijiazhuang050016,China; 3.College of Chemistry,Hebei Normal University,Shijiazhuang050016,China);Study on the Ca~(2+) Binding to Calmodulin by Potential Titration Using a Calcium Ion-Selective Electrode[J];Journal of Instrumental Analysis;2004-04
6 LIU De-long1,2,GENG Li-na1,BAI Juan2,SUN Da-ye2(1.College of Chemistry,Hebei Normal University,Shijiazhuang 050016,China;2.College of Life Science,Hebei Normal University,Shijiazhuang 050016,China);Study on the Binding Equilibrium of Calmodulin-Pb~(2+) by Pb~(2+) Ion-selective Electrode[J];Journal of Instrumental Analysis;2007-02
7 Xing-Hao Liao,Jie Zhao*(Key Laboratory of Ministry of Education for Plant Developmental Biology,College of Life Sciences,Wuhan University,Wuhan 430072,China);Extracellular Calmodulin in Plants and Its Signaling Transduction Pathways[J];Chinese Journal of Cell Biology;2006-05
8 Xiao Fengjuan~(1*), Liu Delong~2, Bai Juan~3, Sun Daye~3 (1. Material Science and Engineering (Department,) Shijiazhuang Railway Institute, Shijiazhuang 050043; 2. Department of Chemistry, (3. Department) of Biology, Hebei Normal University, Shijiazhuang 050091, China);Investigation on Competition Binding of Ca~(2+), La~(3+) and Al~(3+) to Tb_4~(3+)·CaM System by Using Tb~(3+) Fluorescence Probes[J];Journal of The Chinese Rare Earth Society;2004-03
9 Liu Delong1,2,Wang Huijuan1,Bai Juan2,Sun Daye2 (1.College of Chemistry;2.College of Life Science,Hebei Normal University,Shijiazhuang 050016,China);Study on Binding Reaction of Ce~(3+) to Plant Calmodulin by Ce~(3+) Fluorescence[J];Journal of the Chinese Rare Earth Society;2007-05
10 XIAO Feng-juan~1,CHANG Hong~2,LIU De-long~2,BAI Juan~2(1.Department of Materials,Shijiazhuang Railway Institute,Shijiazhuang 050043,China; 2.Hebei Normal Univesity,Shijiazhuang 050016,China);Advance in Study on Interaction of Rare Earth Ions with Calmodium[J];Chinese Rare Earths;2003-06
【Citations】
Chinese Journal Full-text Database 1 Hits
1 LlU De-Long; ZHU Zhi-Hua; ZHAO Shan-Kai; SUN Da-Ye; YANG Yan-Sheng (Department of Chemistry, Analytical and Testing Centert, Zhongshan University, Guangzhou, 510275;Department of Biology, Hebei Normal University, Shijiazhuang, 050016);Matrix-assisted Laser Desorption Ionization Mass Spectrometry for Determination of Molecular Weight of CaMs[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1997-12
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 Ma Ligeng(Department of Biology, Hebei Normal University, 050016,Shijiazhuang, PRC);Light-Regulation of Phosphoenolpyruvate Carboxylase in Plants[J];;1996-02
2 ZHANG Lai-Qun LI Fang SUN Da-Ye (Institute of Molecular Biology, Hebei Normal University, Shijiazhuang 050016);The Effects of Extracellular Calmodulin on Protein Phosphorylation in Cytoplasmic Fraction from Suspension-cultured Tobacco(Nicotiana tobacum L.) Cells[J];Acta Photophysiologica Sinica;2001-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Saijun;ZHANG Wanbai (Dept.Technol.Phys.,Peking Univ.,Beijing,100871) GAO Song;WU Jinguang (Department of Chemistry,Peking Univ.,Beijing,100871) HIDENARI Inoue (Dept.Appl.Chem.,Faculty of Sci.& Technol.,Keio Univ.,Yokahama,223,Japan);Studies on Variable Temperature MCD Spectra A Complex of Chlorophyll-a and Lanthanum[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1996-02
2 GONG Ming YANG Zhonghan CAO Zongxun(T H Tsao)(Life Science College,Peking University,Beijing 10087);Initiating Effects of Calcium on Pollen Germination and Its Regulative Role in Pollen Tube GroWth[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1995-02
3 WANG Libo\ WU Jinguang\ XU Guangxian (College of Chemistry and Molecular Engineering, Peking University, State Key Labortory of Rare Earth Material Chemistry and Application, Beijing, 100871) WANG Caiqing\ ZHANG Tingfang (College of Life Sciences, P;FT IR Study on the Secondary Structure of Earthworm Calmodulin(CaM) and the Effects of Ca 2+ and Tb 3+ Ions[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;1997-06
4 Nie Yuxiu,Zhou Li,Huang Kexin(Department of Histology and Embryology);Regulation of the low dose of samarium chloride on the secretory activity of pancreatic islet cells from diabetes rat[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1997-06
5 Zhang Weifang Liu Dewen(Department of Chemical Engineering, Beijing Institute of Light Industry, Beijing 100037);STUDY ON RARE EARTH IONS LUMINESCENCE AND APPLICATION ON BIOCHEMISTRY[J];JOURNAL OF BEIJING INSTITUTE OF LIGHT INDUSRY;1999-02
6 LU Kuanke; CHANG Zhenzhan; CHEN Baowei; GUO De'an; ZHENG Junhua; WANG Kui (Department of Bioinorganic and Inorganic Drug Chemistry, Beijing Medical University, Beijing 100083);HORMONE-LIKE EFFECT OF RARE EARTH ELEMENTS ON THE PLANT[J];JOURNAL OF BEIJING MEDICAL UNIVERSITY;1997-04
7 Cheng Rong (Ganan work Group of Grassland)Du Guozhen (Gannan school of Animal Husbandry);Dynamic Study of Above-ground Biomass of Herbage Communities in Artificial pastures on Subalpine Meadow[J];;1988-02
8 Zhao Junquan Li Shuan (Yunnan Livestock and Pasture Research Center, Kunming 650212, Yunnan);EFFECTS OF NITROGEN PHOSPHORUS AND POTASSIUM RATES ON BOTH SEED AND DRY MATTER YIELS FOR SETARIA SPHACELATA CV.NAROK[J];Pratacultural Science;1991-02
9 ZHANG Zi he (Gansu Grassland Ecological Institute, Lanzhou Gansu 730020 China);Silent crisis desertification and degeneration of grasasland[J];PRATACULTURAL SCIENCE;2000-02
10 LA Yuan lin (The Supesvisory and Management Station of Guinan County,Qinghai Province,Guinan 813100,China);Prataculture--the fundation of economical development and ecological environment renovation in Qinghai Province[J];Pratacultural Science;2002-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Shen Xuemei(College of Life science,Nanjing Agricultural University,Nanjing 210095);The Kinds of Plant G Protein and the Function of Heterotrimeric G Protein in Cell Signal Transduction[J];Anhui Agricultural Science Bulletin;2009-15
2 ZHOU Xiu-jie1,ZHAO Hong-bo2,HU Zhen-dong3(1.Department of Biology,Huaibei Coal Industry Teachers College,235000,Huaibei,Anhui,China;2.The Attached Middle School,Huaibei Coal Industry Teachers College,235000,Huaibei,Anhui,China;3.Department of Physical Education,Huaibei Coal Industry Teachers College,235000,Huaibei,Anhui,China);The Toxic Study of the High Concentration Rare Earth Nd(NO_3)_3 Etc.[J];Journal of Huaibei Industry Teachers College (Natural Sciences Edition);2005-02
3 LIAO Li-fu1,XIAO Xi-lin1,2,YANG Ming-hui2,YANG Jing1 1.Department of Chemistry and Chemical Engineering,Nanhua University,Hengyang 421001,China 2.State Key Laboratory of Chemo/Biosensing and Chemometrics,Hunan University, Changsha 410082,China;Simultaneous Determination of Calcium and Magnesium by Calculative Spectrophotometric Complexometric Titration[J];Spectroscopy and Spectral Analysis;2007-12
4 WANG Hui-juan,GUO Li-jian,YANG Ning (Hebei College of Industry and Technology,Shijiazhuang 050091, China);Advance in Studies on Calmodulin by Tb~(3+) Luminescence Probe[J];Hebei Chemical Engineering and Industry;2008-08
5 MEI Lin~1 CHENG Zheng-xue~2 SHI Kai-yun~3 1.Logistual Engineering University,Chongqing 400014,China; 2.Chongqing Institute of Optics and Mechanics,Chongqing 400039,China; 3.Chongqing University,Chongqing 400044,China;Progress in the interaction of biological molecules and small molecules by fluorescence spectroscopy[J];Laser Journal;2007-03
6 WANG Chunxia①②(①Nanjing University Technology.Nanjing 210009;②Xu zhou Medical School of Advanced Profession.Xuzhou 221116);Rare-earth element terbium to broad bean root point fresh growingtrend influence[J];Journal of Liaoning Agricultural College;2008-01
7 GAO Li-li1,ZHANG Chen1,QIU Lin1,ZHOU Qing1,2(1.Lab.of Environmental Science,College of Biotechnology,Jiangnan University,Wuxi,Jiangsu 214122,China;2.The Key Lab.of Industrial Biotechnology, Ministry of Education,Jiangnan University,Wuxi,Jiangsu 214122,China);Effects of Lanthanum on Germination of Winter Wheat Seeds under Acid Rain Stress[J];Journal of Triticeae Crops;2008-01
8 MO Zhao-zhan1,CAO Fu-liang2,OU Zu-lan2(1.Department of Chemistry and Biology,Yulin Normal University,Yulin 537000,China;2.College of Forest Resources andEnviroment,Nanjing Forestry University,Nanjing 210037,China);Effects of lanthanum nitrate on growth of Dendrobium fimbriatum tube seedling[J];Journal of Nanjing Forestry University(Natural Sciences Edition);2009-04
9 LIU Shi-quan, ZHONG Tong-sheng, ZHAO Xin-min, XU Ling, DENG Zhong-ri (Department of Chemistry and Environment Engineering, Hunan City University, Hunan Yiyang, 413000, China);Effect of Rare-earth Elements on Dendrobium huoshanense C.Z.Tang et S.J.Cheng[J];Biotechnology;2008-03
10 REN Xiao-xiao2, ZHOU Qing1,2(1.The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;2.Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China);Effects of Rare Earth Elements on Mineral Metabolism in Plant[J];Chinese Journal of Soil Science;2009-02
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 ZHONG Feng ZHAO Shan-Kai(Instrumental Analysis & Research Center, Zhongshan University, Guangzhou, 510275);Matrix-assisted Laser Desorption Mass Spectrometry of Proteins[J];Acta Chimica Sinica;1995-09
2 TONG Zhe Institute of Botany, Academia Sinica, Beijing 100044 LIANG Bo-Fan Hebei University, Baoding 071002;Photomodulated Synthesis of Amaranthin in Amaranthus caudatus Seedlings[J];;1989-01
3 ;Effects of Inhibitors Ca~(2+) and CaM on the Formation of Amaranthin in Amaranthus caudatus Seedlings[J];Plant Physiology Communications;1992-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved