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Studies on Plant Extracellular Calmodulin by Lanthanide Luminescence Probe

LIU De Long 1,* , SUN Da Ye 2, YANG Yan Sheng 3 [WT5”BX](1. Department of Chemistry, 2. Department of Biology, Hebei Normal University, Shijiazhuang 050091, China; 3. Department of Chemistry, Zhongshan University, Guangzhou 510275, China) [WT  
Plant extracellular calmodulin(CaM) has been purified from cauliflower and identified with NAD kinase(NADK) activation and inhibition effect of CaM antagonist W7. Tb 3+ fluorescence titration showed that extracellular CaM contained four metal binding sites. The excitation spectrum and emission specturm indicated that extracellular CaM contained one tyrosine residue which could transfer energy to bound Tb 3+ . Based on Frster type nonradiative energy transfer theory, the distances of Tyr→sites Ⅲ, Ⅳ have been determined, these are 1 104 nm(Tyr→Ⅲ, site) and 1 056 nm(Tyr→Ⅳ, site). By studing the effect of CaM antagonist W7 and CaM antibody on Tb 3+ sensitized fluorescence, it was found that the binding sites of W7 and antibody were located on the c terminal part of plant extracellular CaM which contains domain Ⅲ and domain Ⅳ. [WT5HZ]
【Fund】: 国家自然科学基金!重点项目 (批准号 :3 973 0 2 3 0 );; 中国科学院长春应用化学研究所稀土化学与物理开放实验室资助
【CateGory Index】: O614
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