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《Chemical Research In Chinese Universities》 2000-12
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Coupling of DNA to Bead Type Cellulose as Immunoadsorbent

KONG De Ling *, DAI Jun, CHEN Chang Zhi, YU Yao Ting(Institute for Molecular Biology, Bioactive Materials Key Laboratory of Ministry of Education, Nankai University, Tianjin 300071, China) [WT5HZ]  
In order to remove the anti DNA antibody from the blood of patients with systematic lupus erythematosus(SLE), DNA immunoadsorbent was prepared by coupling calf thymus DNA to epichlorohydrin activated cellulose beads. The methods and conditions for activation of carrier beads, DNA coupling and adsorption with patient's plasma were investigated. The activation level was found to be dependent on the NaOH concentration, amount of offered epichlorohydrin and the molar ratio between NaOH and epichlorohydrin. One milliliter of cellulose beads was activated by incubation with 0.5 mL of epichlorohydrin and 2.0 mL of 2.0 ? ?.0 mol/L NaOH solution at 40 ℃ for 2 h, and the activation level was 60_100 μmol/mL beads. DNA coupling was found to be strongly dependent on temperature and pH. The optimal coupling conditions were 0.1 mol/L Tris HCl buffer, pH 7.9 ? ?.0 and 70—90 ℃. High activation level, high DNA concentration and long reaction time were also required. Under the optimal condition, 2.0 ? ?.0 mg of DNA could be coupled to 1.0 mL of cellulose beads. In vitro adsorption tests showed that incubation of 1.0 mL of adsorbent with 3.0 mL of plasma could remove 40%—70% of anti DNA antibody from the plasma. [WT5HZ]
【Fund】: 国家自然科学基金! (批准号 :2 96740 14 )资助
【CateGory Index】: O636;R318
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【References】
Chinese Journal Full-text Database 10 Hits
1 YAN Wei-Rong, YU Yao-Ting *, YANG Li, ZHANG Wei-Hua, MIAO Peng-Chong(The Key Laboratory of Bioactive Material of Minstry of Education, Nankai University, Tianjin 300071, China);Studies on New Immunoadsorbent for Myasthenia Gravis[J];Chemical Research In Chinese Universities;2002-10
2 CHU Jia Qiang 1, YU Yao Ting \{1*\}, ZHANG Wei Hua 1, CHEN Jian Jun 1, ZHU Bo Ru 1, LIU Jing Duo 2 (1. Key Lab of Bioactive Materials Research of Educational Ministry, Nankai University, Tianjin 300071; 2. Air Force Military General Hospital, Beijng 100035, China);In vitro Studies on the Immunoadsorbent for Removal of IgA in IgA-Nephropathy (Ⅱ)[J];Chemical Research In Chinese Universities;2003-04
3 DONG Qi Gong *, ZHANG Jun Ping, LI Ya Rong, JIA Ying Qi (Department of Chemistry, Northwest University, Xian 710069, China);Synthesis and Adsorption Properties of New Type Chelating Cellulose Resins Containing Sulfur and Nitrogen[J];Chemical Research In Chinese Universities;2003-04
4 Huang Jianhui 1 Liu Minghua 1 Fan Juan 2 Zhan Huaiyu 2 (1.Dept. of Environment and Resources Engineering, Fuzhou University,Fuzhou 350002; 2.State Key Laboratory of Pulp & Paper Engineering, South China University of Technology, Guangzhou 510640);Research and Application of the Cellulose Adsorbents[J];Guangdong Pulp & Paper;2004-01
5 ZOU Chang-jun (School of Chemistry and Chemical Engineering, Southwest Petroleum Institute, Chengdu 610500,China);Coupling of Anti-HBsAg Monoclonal Antibody to Bead Type Cellulose as Immunoadsorbent[J];;2004-01
6 SHI Qing-hong, PENG Guan-ying, SUN Shu, SUN Yan (Department of Biochemical Engineering, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China);Process Intensification and Characterization of Agarose Gel Activation with Epichlorohydrin[J];The Chinese Journal of Process Engineering;2007-04
7 ZOU Chang jun 1,TAN Nai di 2,YUE Chang jin 2 (1.School of Chemistry and Chemical Engineering, Southwest Petroleum Institute, Nanchong 637001,China;2.Department of Pharmacy and Applied Chemistry, Jilin Institute of Chemical Technology, Jilin 132022;Coupling of Anti-HBsAg Monoclonal Antibody as Well as Immunoadsorption[J];Fine Chemicals;2002-05
8 YIN Chunyan YU Yaoting WANG Yongjian LIN Enfu CHEN Changzhi The Key Laboratory of Bioactive Materials,Ministry of Education,Nankai University,Tianjin 300071,China;PREPARATION AND PROPERTIES OF DNA CARBONIZED RESIN AS ADSORBENT FOR REMOVAL OF RHEUMATOID FACTOR[J];Ion Exchange and Adsorption;2005-06
9 GAO Po1,YUE Zheng-yu1,YAN Xin1,FANG Gui-zhen2(1.School of Chemistry and Materials Science,Heilongjiang University,Harbin 150080,China;2.College of Material Science and Engineering,Northeast Forestry University,Harbin 150040,China);The adsorbability of diethylenetriamine oxycellulose for creatinine and As(Ⅲ)[J];Journal of Natural Science of Heilongjiang University;2010-01
10 CHENG Shuang 1,2 ( 1Department of Food Engineering, 2Department of Chemistry,Liaocheng Teachers'Univeristy ShanDong Liaocheng 252059);Immobilization of Neutral Proteinase AS1.398 with Extrusion Soybean Fiber[J];;2002-04
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Yang Yan, Li Naihong and He Binglin(Department of Chemistry, Nankai University, Institute of Molecular Biochemistry, Nankai University, Tianjin)Quan Wulie, Cui Runhai, Shao Xinhua, Ye Fuhua, Qian Shaocheng and Song Jichang (Liter and Biliary Disease Research Unit. Hedong Hospital, Tianjin);Removal of DNA Antibodies and DNA Immune Complexes from Mammal Blood with Spherical Carbonaceous Polymeric Adsorbent by Hemoperfusion[J];Chemical Research In Chinese Universities;1985-09
2 Zhu Boru; Shi Zuoqing; He Binglin(Institute of Polymer Chemistry, Nankai University, Tianjin 300071);STUDY ON THE PREPARATION OF SPHERICAL MACROPOROUS CELLULOSIC IONEXCHANGER AND ITS ADSORPTION PROPERTIES (Ⅰ) THE PREPARATION OF SPHERICAL MACROPOROUS CELLULOSE[J];ION EXCHANGE AND ADSORPTION;1996-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ;Adsorbents for Hemoperfusion[J];Materials Review;1995-03
2 HE Bing-Lin; MA Jian-Biao(The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation,Institute of Polymer Chemistry, Nankai University, Tianjin, 300071);Polymeric Adsorbents for Blood Purification[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1997-07
3 TANG Ai\|min, LIANG Wen\|zhi (State Key Laboratory of Pulp and Paper Engineering,South China University of Technology,Guangzhou 510640,China);The Functionalization of Cellulose[J];POLYMER BULLETIN;2000-04
4 ZOU Chang-jun (School of Chemistry and Chemical Engineering, Southwest Petroleum Institute, Chengdu 610500,China);Coupling of Anti-HBsAg Monoclonal Antibody to Bead Type Cellulose as Immunoadsorbent[J];;2004-01
5 LING Hong-xia1 CHEN Zhong-lan2(1.Dept of Biological and Chemical Engineering,Pan zhi hua 617000,China;2.Chemistry and ChemicaI Engineering,China West Normal University,Nanchong 637002,China);Preparation and property of porous spherical cel iulose sorbent[J];Chemical Research and Application;2007-08
6 He Yongbing; Meng Lingzhi; Chen Fujie; Wu Chengtai (Department of Chemistry, Wuhan University, Wuhan 430072)Tang Yuirong (Department of Analysis-Measurement Science, Wuhun University, 430072);COTTON STALK CELLULOSE MODIFICATION(Ⅱ) ──SYNTHESIS AND ADSORPTION PROPERTIES FOR METALLIC ION OF AZACROWN ETHER CELLULOSE DERIVATIVES[J];ION EXCHANGE AND ADSORPTION;1998-03
7 DAI Jun1 CHEN Changzhi1 YU Yaoting1 SUN Yumin2 1 Institute for Malecular Biology, Bioactive Materials Research Laboratory, Nankai Universty, Tianjim 300071, China 2 Institute for traditional Chinese Medicine, Tingjin;PREPARATIN AND PROPERTIES OF IMMUNOADSORBENT FOR RHEUMATOID FACTORS[J];Ion Exchange and Adsorption;2001-05
8 Guo Xianquan;He Binglin (Institute of polymer Chemistry Nankai University, Tianjin, 300071);Recent Advances in Clinical Application of Active polymer On Hemoperfusion[J];Ion Exchange and Adsorption;1995-04
9 OUYANG Zhi-gang,CHEN Zhong-lan,FENG Li(Lab of Applled Chesnistry and Pollution Contral,China West Normal University,Nanchong 637009,China);Porous Spherical Celiulose Preenrichment for Determination of Cu by Flame Atomic Absorption Spectrometry[J];Journal of China West Normal University(Natural Sciences);2009-04
10 QIN Juan,ZHANG Yong-gang,FU Xue-qi,XU Xiang(College of Environmental Science and Engineering,Nankai University,Tianjin 300071,China);The research progress of spherical cotton cellulose[J];Tianjin Chemical Industry;2006-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WU Gang,SHEN Yu-Hua,XIE An-Jian,LIN Hong-Yun(College of Chemistry and Chemical Engineering, Anhui University, Heifei230039,China);Studies on the adsorption of bilirubin in solution by chitosan[J];Journal of Anhui University(Natural Sciences);2003-01
2 WEN Han (Department of Bioengineering,Anhui Agricultural University,Hefei 230036);Purification of RGL from Rice Germ by Affinity Chromatograph on a Chitin Column[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2001-02
3 TIAN Sen lin, ZHANG Qi hua & GUAN Yan wei (Faculty of Forestry Engineering, Southwest Forestry College, Kunming 650224, China);A STUDY OF THE SAW-DUST ADSORPTION EFFECT IN PROCESSING WASTE WATER CONTAINING CHROMIUM[J];Journal of Safety and Environment;2002-04
4 LIU Xiaopeng ZHANG Jianbo WANG Weijing LI Feng (Department of Environmental Science, College of Environmental Scienc e, Peking University, Beijing,100871);Adsorption of Cr(Ⅵ) to Prepared Chitosan Ramifications[J];Acta Scicentiarum Naturalum Universitis Pekinesis;2003-06
5 CHEN Man~1, REN Qi-sheng~2, SONG Xin-rong~2, FENG Chang-gen~1(1.School of Mechatronics Engineering, Beijing Institute of Technology, Beijing100081, China; 2.Beijing Jiangzhong Zesheng Science and Technology Co.Ltd., Beijing100050, China);Preparation and Application of Affinity Chromatography Resin Immobilized Erythrina Trypsin Inhibitor[J];Journal of Beijing Institute of Technology;2005-02
6 YANG Li-zhen,HAO Yan-ping,JIA Jing-ru,HAN Xing-zhou(Beijing Institute of Graphic Communication,Beijing 102600,China);Magnetic Liquid made in chemical co-precipitation Method[J];Journal of Beijing Institute of Graphic Communacation;2005-04
7 LIU Yu lan WANG Juan ZHANG Ji ping (Medical College,Qindao University,Qindao,Shandong,266021);The Skin Moisturizer and Evaluation of Its Propertis[J];CHINA SURFACTANT DETERGENT & COSMETICS;1999-05
8 YAN Jin-long (Chemical Engineering Department, Yancheng Engineering College,Yancheng,P.R.China 224003).;The Spetrophotometric Determination of Micro Chromium(VI) Enriching on Protoated Chitin[J];Materiais Protection;2001-11
9 YANG Ming-ping, LI Guo-bin(Department of Chemical Engineering, Xiangtan Polytechnic University, Xiangtan 411201, China);Adsorbing of Minim Chromium(Ⅵ) in Wastewater by Chitosan[J];Materials Protection;2003-12
10 ;The Progress of the Study on New Type Immunoadsorbents[J];Materials Review;1994-05
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 AN Fu-qiang*,FENG Xiao-qin and ZHANG Li-ping(Chemical Department, North University of China,Taiyuan 030051);Studies on adsorption properties of composite and chelating adsorption material of PEI/SiO_2 for Cu~(2+)[J];Chinese Journal of Analysis Laboratory;2007-02
2 CHU Jia-Qiang,ZHANG Wei-Hua,YU Yao-Ting *,ZHU Bo-Ru,CHEN Chang-Zhi (State Key Lab of Bioactive Materials Research,Nankai University,Tianjin 300071,China);In Vitro Studies of the Immunoadsorbent for Removal of IgA in IgA-nephropathy (Ⅲ)[J];Chemical Research In Chinese Universities;2004-08
3 Huang Jianhui 1 Liu Minghua 1 Fan Juan 2 Zhan Huaiyu 2 (1.Dept. of Environment and Resources Engineering, Fuzhou University,Fuzhou 350002; 2.State Key Laboratory of Pulp & Paper Engineering, South China University of Technology, Guangzhou 510640);Research and Application of the Cellulose Adsorbents[J];Guangdong Pulp & Paper;2004-01
4 YANG Fafu,CHEN Xilei,GUO Hongyu,CAI Xiuqin(College of Chemistry and Materials Science,Fujian Normal University,Fuzhou 350007);SUPRAMOLECULAR CHELATING RESINS——DESIGN,SYNTHESES AND ADSORPTION PROPERTIES OF CELLULOSE GRAFTED WITH CALIX[4]ARENE[J];Acta Polymerica Sinica;2005-06
5 ZHANG Jian-da,YUAN Zhi(The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation,Institute of Polymer Chemistry,Nankai University,Tianjin 300071,China);Progress in Hemoperfusion Material[J];Chinese Polymer Bulletin;2006-04
6 YANG Lian-min~1,MO Bin-rong~2,CHEN Wen-na~1,CHEN Yuan-xia~2,KE Min~2(1.Department of Chemistry,Guangxi Teachers College,Nanning 530001, China;2.Guangxi Research Institute of Chemical Industry,Nanning 530001,China);Preparations of Cellulose Adsorbents Contained Sulfur and Nitrogen and its Adsorption Properties[J];Technology & Development of Chemical Industry;2006-09
7 YANG Lian-min1,CHENG Wen-na2 (1.Luizhou Medical College,Luizhou 545006,China;2.Department of Chemistry,Guangxi Teachers Education University,Nanning 530001,China);On Preparation and Absorption Property of Modified Sugarcane Bagasse Cellulose Water Treatment Chemicals[J];Journal of Guangxi Teachers Education University(Natural Science Edition);2008-03
8 Qiu Huidong1,2,Duan Chuanren1(1.Bioengineering College,Chongqing University,Chongqing 400044 China; 2.Department of Chemical and Biological Engineering,Chongqing Institute of Science and Technology, Chongqing 400050,China);Research on the application of agricultural waste by-product to the treatment of industrial wastewater[J];Industrial Water Treatment;2007-01
9 ZHAO Ping,WANG Ya,ZHANG Yi,WANG Ling,LIN Ying-ji(College of Life Science and Engineering,Lanzhou Univ.of Tech.,Lanzhou 730050,China);Study of adsorbability of modified fiber of buckwheat husk and its effects of lead removal[J];Journal of Lanzhou University of Technology;2009-05
10 LIU Xi-lian,LIN Ri-jia,CHEN Shui-xia (PCFM Lab,Materials Science Institute,Sun Yat-Sen University,Guangzhou 510275,China);Preparation of a bagasse-based strong base anion exchange fibers and their application in sugar colorants removal[J];Materials Research and Application;2010-04
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