Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chemical Research In Chinese Universities》 2001-12
Add to Favorite Get Latest Update

Mechanism of Heavy Metal Ions on α-Amylase Activity from Porcine Pancreas

HONG Fa\|Shui\+*, WANG Xue\|Feng, WU Kang, SHEN Song\|Dong, SU Guo\|Xing,PAN Xin\|Fa (Department of Biology Science, College of Life Science, Suzhou University, Suzhou 215006, China)  
The activity of \%α\%\|amylase from porcine pancreas was enhanced under the treatment by Ce\+\{3+\}, Cd\+\{2+\} and Pb\+\{2+\} at a low concentration (0.5\_5 μmol/L) , but was inhibited by Ce\+\{3+\}, Cd\+\{2+\} and Pb\+\{2+\} at a high concentration(4 or 5 μmol/L above). Ce\+\{3+\}, Cd\+\{2+\} and Pb\+\{2+\} at a high concentration could competitively displace Ca\+\{2+\} from \%α\%\|amylase. The equilibrium dialysis demonstrated that \%α\%\|amylase have five Ca\+\{2+\}\|binding sites with different affinities. The fluorescence titration showed that Ce\+\{3+\}, Cd\+\{2+\} and Pb\+\{2+\} are not only bound to Ca\+\{2+\}\|binding sites, but also bound to other sites of \%α\%\|amylase, and resulted in the \%α\%\|amylase conformational changes.
【Fund】: 苏州大学人才引进基金 (批准号 :XQ316 0 11)资助
【CateGory Index】: O629
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 QIN Tao et al (Enzyme Engineering Institute,Shaanxi Academy of Sciences,Lintong,Shaanxi 710600);Research Reviw of Compound Feed Additive Ingredient[J];Journal of Anhui Agricultural Sciences;2008-04
2 GUO Yong-jun et al (Tianjin Key Lab of AQUA-Ecology and Aquaculture,Department of Fishery Science,Tianjin Agricultural University,Tianjin 300384 );Effects of Metal Ions on Protease Activities in the Intestines and Hepatopancreas of Red-white Ornamental Carp[J];Journal of Anhui Agricultural Sciences;2010-10
3 MA Cheng et al(The Key Laboratory of Animal Biology,Engineering Research Center of Bioactive Substances,College of Life Sciences,Chongqing Normal University,Chongqing 400047);Study on Amylase Activities of the Hepatopancreas in Cultured Tinca tinca L.[J];Journal of Anhui Agricultural Sciences;2012-07
4 BAI Dong-qing1,2 LIU Jin-lan1,2 YAN Shan-shan1,2 GUO Yong-jun1,2 WU Xuan1,2 TANG Fei1,2(1Department of Fishery Science,Tianjin Agricultural University,Tianjin 300384;2 Key Lab of Aqua-Ecology and Aquaculture of Tianjin);Effects of Four Kinds of Metal Ions on Amylase Activities in Red-white Ornamental Carp[J];;2009-19
5 NING Bo WU Xuan JIA Zhi-chao BAI Dong-qing QIAO Xiu-ting LIANG Guang-hai(Tianjin Key Lab of AQUA-Ecology and Aquaculture,Department of Fishery Science,Tianjin Agricultural University,Tianjin 300384);Effects of Four Kinds of Metal Ions on Amylase Activities in Digestive Organ of Chalcalburnus chalcoides aralensis[J];Modern Agricultural Science and Technology;2012-09
6 QI Ye1,SUN Xiang-jun2,LIANG Yong-jun2,QIAO Xiu-ting1(1.Tianjin Key Laboratory of Aqua-ecology and Aquaculture,Fishery Science Department,Agricultural University,Tianjin 300384,China;2.Beijing Fisheries Research Institute,Beijing 100068,China);Effects of Four Metal Ions on Protease Activity in Jade Perch(Scortum barcoo)[J];Fisheries Science;2010-09
7 CHEN Hui1 WANG Cuiju1 WU Pengwei1 DI Keqian2 BAO Huiling3 HUANG Renlu1 (1.College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071001,China; 2.Hebei University,Baoding 071001,China;3.Shijiazhuang Municipal Animal Husbandry and Aquatic Products Bureau,Shijiazhuang 052160,China);Effects of Nano-Ceria on Activity and Gene Expression of Pancreatic Lipase in Hens[J];Chinese Journal of Animal Nutrition;2012-05
8 HONG Fa-shui College of Life Sciences, Suzhou University,Suzhou 215006,China;Study of the Effect of Pb ~(2+) on α-Amylase Activity by Spectroscopy[J];Spectroscopy and Spectral Analysis;2003-03
9 JIANG Hui-min;Key Laboratory of Animal Physiology and Biochemistry in Shandong;;Effect of Ammonia on Activity of Amylase and Lipase in Carps[J];Progress in Veterinary Medicine;2013-10
10 LUO Lin-tao;TONG Wei;HAN Ji-chang;WANG Huan-yuan;XIE Jian-cang;MAZeng-hui;Institute of Water Resources and Hydro-electric Engineering,Xi'an University of Technology;Key Laboratory of Degraded and Unused Land Consolidation Engineering,theMinistry of Land and Resources of China,Shaanxi Province Land Reclamation Engineering Technology Research Center,Shaanxi Estate Development Service Corporation;;Analysis and evaluation of heavy metal content in the arsenic rock,sand and compound soil of Mu Us Desert[J];Journal of Safety and Environment;2014-01
【Citations】
Chinese Journal Full-text Database 10 Hits
1 HONG Fa-shui;FANG Neng-hu;ZHAO Gui-wen;(Department of Chemistry. University of Science and Technology of China, Hefei 230026; 2. College of Chemical Industrial,University of Communication of Shanghai,Shanghai 200240);Studies of physiological and biochemical bases of Lanthanum on increasing seed vigor of rice[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1999-04
2 ZHU Bing, DU Xiu Lian, LI Rong Chang, WANG Kui  (Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China) JIN Jian , WANG Bo Cheng ( National Key Laboratory of Nuclear Medicine, Wuxi 214063, Chin;Spectroscopic Studies on the Binding of Lanthanides to the Apolactoferrin[J];Chemical Research In Chinese Universities;2001-01
3 Duan Ch-angqua, Wang Huanxiao, Qu Zhongxiang (Biology Department, Yu nnan University, Kunming 650091);Studies on the Effects of Heavy Metals on the Contents of Nucleic Acids and Activities of Nu-cleases in the Root Tips of Vicia faba.[J];Environmental Science;1992-05
4 Duan Changqun Wang Huanxiao(Biology Department & Evolutionary Ecology Laborotary,Yunnan University,Kunming,650091,China);THE EFFECTS OF LEAD,CADMIUM AND MERCURYIONS ON LDH IN Vicia faba[J];ACTA ECOLOGICA SINICA;1998-04
5 Li Xin-Min;Ni Jia-Zuan (Laboratory of Rare Earth Chemistry and Physics,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022)Wang Ping-Yuan;Chen Jian-Wen;Hwang Fen (National Laboratory of Biomacromolecules,Institute of Biop;Effects of Rare Earths on the Activity of Sarcoplasmic Reticulum Ca ̄(2+)-ATPase[J];;1995-03
6 Wang Weilie,Tu Chuqiao,Wang Guanghui(Institute of Chemistry,Wuhan University,Wuhan 430072);Advance in Study of Interaction Between Rare Earth Metal Ions and Enzyme Molecules[J];CHINESE RARE EARTHS;1998-03
7 Hong Fashui,Fang Nenghu,Gu Yuehua,Zhao Guiwen (University of Science and Technology of China,Hefei 230026);Effect of Cerium Nitrate on Seed Vigor and Activitiesof Enzymes during Germination of Rice[J];CHINESE RARE EARTHS;1999-03
8 Wu Jiayan, Xia Zenglu, Ba Yin and Li Senzhao (Institute of geography, Academia Sinica, Beijing 100101).;Enzymological diagnoses of soil pollution by heavy metals: influence of Cd, Cu, Pb and As in purple soil on dehydrogenase of rice roots.[J];Chinese Journal of Applied Ecology;1991-01
9 Ma Chengcang, Hong Fashui and Li Qingfang(Huaibei Teachers College of Coal Industry,Huaibei 235000).;Effect of seed soaking with Hg on enzyme activities of maize seed during its germination.[J];CHINESE JOURNAL OF APPLIED ECOLOGY;1997-01
10 Ma Chengcang;Hong Fashui;Li Qingfang (Department of Biology, Huaibei Teachers College);THE EFFECT OF MERCURY ON METABOLISMAND ENZYMES ACTIVITIES OF SOYBEAN SEED DURING GERMINATION[J];;1995-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HU Yunhu Lu Xianwen(Department of chemistry and biology,Huainan Normal University,Huainan 232001,China);Influence of Heavy metals zinc on wheat chlorophyll synthesis[J];Anhui Agricultural Science Bulletin;2008-09
2 Cao Deju et al (Dept. of Environment and Plant Protection, Anhui Agricultural University,Hefei 230036);Effect of Mercury Actate on Root Tip Cell of Horse Bean[J];Journal of Anhui Agricultural Sciences;2003-03
3 XIONG Yu-hui et al (School of Biological Science,Huzhou Teachers College,Huzhou,Zhejiang 313000);Review of the Toxic Effects of Cadmium on Plants and Their Tolerant Mechanism[J];Journal of Anhui Agricultural Sciences;2006-13
4 LIU Wan-ling (Biology Department of the Shangqiu Normal College, Shangqiu, Henan 476000);Heavy Metal Pollution and its Effect on Plant Growth and Development[J];Journal of Anhui Agricultural Sciences;2006-16
5 LI Xiu-zhen et al(Chonging Academy of forestry,Chongqing Key Laboratory of the Three Gorges Storehouse District Forest Ecodogy Protects and Restores,Chongqing 400036);Effects of Heavy Metal on Plant Growth and Quality[J];Journal of Anhui Agricultural Sciences;2008-14
6 LU Xian-wen et al(Huainan Normal University,Huainan,Anhui 232001);Effect of Heavy Metals Cu and Zn on Seed Germination and Biomass of Wheat[J];Journal of Anhui Agricultural Sciences;2008-35
7 TIAN Shuai et al (School of Geographical Sciences,Guangzhou University,Guangzhou,Guangdong 510006);Study on the Response of Maize to Heavy Metal Stress and Its Applications in Phytoremediation[J];Journal of Anhui Agricultural Sciences;2009-05
8 HUANG Kai-feng et al(Institute of Plant Genetics and Breeding,School of Life Sciences,Guizhou Normal University,Guiyang,Guizhou 550001);Effects of Cd and Pb Combined Stress on the Antioxidant Enzyme of Zizania latifolia[J];Journal of Anhui Agricultural Sciences;2009-19
9 XUE Luo-qi et al (College of Agriculture,Nanjing Agricultural University,Nanjing,Jiangsu 210095);Effects of Cd Stress on Physiological and Biochemical Traits and Quality of Maize[J];Journal of Anhui Agricultural Sciences;2009-24
10 GONG Hong-mei et al (Biological Department,Resources and Environment College of Henan Polytechnic University,Jiaozuo,Henan 454000);Research Progress on the Toxicity of Zinc to Plants and Its Mechanism[J];Journal of Anhui Agricultural Sciences;2009-29
China Proceedings of conference Full-text Database 3 Hits
1 LIU Hui-fen, GAO Da-xiang, MA Yong (Department of Agronomy, Tianjin Agricultural College, Tianjin 300384, China);Effects of Hg Stress on Growth and Physiological and Biochemical Characteristics of Rice Seedlings[A];[C];2007
2 Chen Zuyi (Naning Agricultural University, Nanjing, 210095, China);Hormesis-Effects of Rare Earths on Biologys and Its Concealed Effects on Agricultural Eco-Environment[A];[C];2004
3 ZHANG Jun 1,LI Min 1,YUAN Zhuo-bin 2 1.School of Chemistry and Engineering,Inner Mongolia University,Hohhot 010021,China 2.School of Chemistry and Chemical Engineering,University of Chinese Academy of Sciences,Beijing 100049,China;The Effect of lanthanum nitrate on the Activity of the Hind III[A];[C];2013
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 SONG Qing-ping,DING Chun-mei(Dept.of Bioch.Engn.,Anhui University of Technology and Science,Wuhu 241000,China);Study on antibacterial properties of microsphere-chitosan Silver complex[J];Journal of Anhui University of Technology and Science(Natural Science);2010-01
2 YANG Li-ling et al (Anyang Institute of Technology,Anyang,Henan 455000);Research Advance in the Assessment of Phosphorus Form in Soil[J];Journal of Anhui Agricultural Sciences;2006-19
3 WANG Kun et al(Fisheries College & Institute of Aquaculture Biotechnology,Jimei University,Xiamen,Fujian 361021);Effects of Different Temperatures and pH on the Protease and Amylase Activities in the Digestive Tract of Anguilla rostrata[J];Journal of Anhui Agricultural Sciences;2007-33
4 LI Xiao-hui et al(Pearl River Fisheries Research Institute Chinese Academy of Fisher Science,Guangzhou,Guangdong 510380);Effects of Astaxanthin on the Color of Blood Parrot[J];Journal of Anhui Agricultural Sciences;2008-20
5 HUI Ming et al(College of Biological Engineering,Henan University of Technology,Zhengzhou,Henan 450001);Advances in Application Research of Bacillus subtilis[J];Journal of Anhui Agricultural Sciences;2008-27
6 LIU Na et al (College of Bioengineering,Henan University of Technology,Zhengzhou,Henan 450001);Effects of Different Concentrations of Metal Ions on the Acid Protease Activity[J];Journal of Anhui Agricultural Sciences;2010-32
7 HEI Shu-mei(College of Life Science,Yanan University,Yanan,Shaanxi 716000);Effects of Heavy Metal-Chromium on the DNA Methylation in Wheat Seedling[J];Journal of Anhui Agricultural Sciences;2011-13
8 CHU Qing-long et al(College of Life Sciences,Henan Agricultural University,Zhengzhou,Henan 450002);Screening and Breeding of High-yield Strains for Acid α-Amylase by UV Mutagenesis[J];Journal of Anhui Agricultural Sciences;2011-13
9 ZHANG Sheng-zhou,ZHU Lian-jiu,QIAN Yu,CHENG Wen-juan(Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province,College of Life Science,Anhui Normal University,Wuhu 241000,China);Effects of pH and Temperature on the Activity of Lipase in Digestive Tract of Rana Nigromaculata[J];Journal of Anhui Normal University(Natural Science);2008-02
10 ZHU Ming-jun,LIANG Shi-zhong (College of Food and Biological Engineering, South China University of Science and Technology, Guangzhou 510640);Effects of Liquid Volume and Inoculum Concentration in Flasks on Growth and Astaxanthin Accumulation of Phaffia rhodozyma[J];Amino Acids & Biotic Resources;2002-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 GUO Yong-jun et al (Tianjin Key Lab of AQUA-Ecology and Aquaculture,Department of Fishery Science,Tianjin Agricultural University,Tianjin 300384 );Effects of Metal Ions on Protease Activities in the Intestines and Hepatopancreas of Red-white Ornamental Carp[J];Journal of Anhui Agricultural Sciences;2010-10
2 LIU Na et al (College of Bioengineering,Henan University of Technology,Zhengzhou,Henan 450001);Effects of Different Concentrations of Metal Ions on the Acid Protease Activity[J];Journal of Anhui Agricultural Sciences;2010-32
3 LIU Ya-qin et al(School of Bioscience and Engineering,The North University for Ethnics,Yinchuan,Ningxia 750021);Optimization of the Fermentation Condition for Acid-resistant α-amylase Producing Strain[J];Journal of Anhui Agricultural Sciences;2010-35
4 LIU Jie et al(Honghe University,Mengzi,Yunnan 661100);Content Analysis of Metal Elements in Wild Caragana sinica on Honghe[J];Journal of Anhui Agricultural Sciences;2011-21
5 LIANG Jia et al (College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083);Cytotoxicity research of Recombinant Human Lactoferrin on Primary Hepatocyte and Nephrocyte Cell[J];Journal of Anhui Agricultural Sciences;2011-36
6 MA Cheng et al(The Key Laboratory of Animal Biology,Engineering Research Center of Bioactive Substances,College of Life Sciences,Chongqing Normal University,Chongqing 400047);Study on Amylase Activities of the Hepatopancreas in Cultured Tinca tinca L.[J];Journal of Anhui Agricultural Sciences;2012-07
7 BAI Dong-qing1,2 LIU Jin-lan1,2 YAN Shan-shan1,2 GUO Yong-jun1,2 WU Xuan1,2 TANG Fei1,2(1Department of Fishery Science,Tianjin Agricultural University,Tianjin 300384;2 Key Lab of Aqua-Ecology and Aquaculture of Tianjin);Effects of Four Kinds of Metal Ions on Amylase Activities in Red-white Ornamental Carp[J];;2009-19
8 NING Bo WU Xuan JIA Zhi-chao BAI Dong-qing QIAO Xiu-ting LIANG Guang-hai(Tianjin Key Lab of AQUA-Ecology and Aquaculture,Department of Fishery Science,Tianjin Agricultural University,Tianjin 300384);Effects of Four Kinds of Metal Ions on Amylase Activities in Digestive Organ of Chalcalburnus chalcoides aralensis[J];Modern Agricultural Science and Technology;2012-09
9 JIANG Ai-li,YU Zhen,WANG Chang-hai(School of Ocean,Yantai University,Yantai,Shandong 264005,China);Effect of Pb~(2+) on Metabolism of the Ascidian Styela Clava[J];Transactions of Beijing Institute of Technology;2011-02
10 QI Ye1,SUN Xiang-jun2,LIANG Yong-jun2,QIAO Xiu-ting1(1.Tianjin Key Laboratory of Aqua-ecology and Aquaculture,Fishery Science Department,Agricultural University,Tianjin 300384,China;2.Beijing Fisheries Research Institute,Beijing 100068,China);Effects of Four Metal Ions on Protease Activity in Jade Perch(Scortum barcoo)[J];Fisheries Science;2010-09
China Proceedings of conference Full-text Database 1 Hits
1 Wang Cuiju~1,Liu min~2,Chen Hui~1,Huang Renlu~1,Wu Pengwei~1 (1.College of Animal Science and Technology,Agricultural University of Hebei,BaoDing,071001; 2.Hebei North University,Zhangjiakou,075000);Effect of nano-ceria on growth and digest organs development of rearing hens[A];[C];2011
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Guang-Hui;HUA Jian-Li(Department of Chemistry, Wuhan University, Wuhan, 430072);Catalyzed Oxidation by Rhus Vernicifera Laccaxe(X)──The Laccase-catalyzed Oxldation of Cateckols Containing tert-Butyl Group and the Microenvironment Effect of Rhus Laccase[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1995-11
2 YANG Bin Sheng * (Institute of Molecular Science, Shanxi University, Taiyuan, 030006) Wesley R. Harris (Department of Chemistry, University of Missouri St Louis, St Louis, MO63121, USA);Spectroscopic Studies on the Binding of Lanthanides to Apoovotransferrin[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1998-07
3 Huang Yinxiao Lin Shunhua Han Rongzhang Yao Yiqun (Institute o] Botany, Academia Sinica);THE EFFECT OF DIFFERENT MERCURY COMPOUNDS ON RICE AND WHEAT AND THEIR ACCUMULATION IN CROPS[J];Acta Ecologica Sinica;1985-04
4 Lin Yuefu, Chen Rongzhong, Zhou Xiulan, Yin Weiping and Wang Chusheng (Third Institute of Oceanography, NBO, Xiamen);EFFECTS OF MERCURY ON THE GPT ACTIVITY OF PERNA VIRIDIS[J];Journal of Oceanography In Taiwan Strait;1985-01
5 Wang Guanghul; Jiang Tao(Department of Chemistry, Wuhan University, Wuhan, China, 430072);CATALYZED OXIDATION BY RHUS VERNICIFERA LACCASE Ⅵ. STUDIES ON MECHANISM OF ENZYMATIC OXIDATION REACTION BY RHUS LACCASE[J];WUHAN UNIVERSITY JOURNAL(NATURAL SCIENCE EDITION);1994-01
6 Zhang Baolin Wang Wenqing (Technical Physics Department of Peking University, Beijing. 100871);INTERACTION OF RARE EARTHS WITH BOVINE SERUM ALBUMIN[J];Chinese Journal of Inorganic Chemistry;1993-04
7 Wu Zhaoming;Tang Xie;Jia Zhewang;wang Baokui Institute of Botany,Academia Sinica Xinjian Academy of Agriculture;STUDIES ON THE EFFECT OF RARE EARTH ELEMENTS ON THE INCREASEMENT OF YIELD IN AGRICULTURE 2. THE EFFECTS OF RARE EARTH ELEMENTS ON SOME PHYSIOLOGICAL PROCESSES OF CROPS[J];Journal of The Chinese Rare Earth Society;1984-02
8 Yang Jiapu and Zhang Shuyuan(Shanxi Agricultural University);PRELIMINARY STUDY OF THE EFFECT OF RARE EARTH ELEMENTS ON IMPROVING THE RESISTANCE OF WHEAT TO ADVERSITY[J];Journal of The Chinese Rare Earth Society;1986-04
9 Liu Enxia(Department of Biology, Inner Mongolia Normal University, Hohhot 010022);Influences of Rare Earths on Sprouting and Root System Growth of Helianthus Annuus Linn[J];CHINESE RARE EARTHS;1996-03
10 Yao Kemin Chen Jianshe (Department of Chemistry);Stability Studies of lanthanide Complexes with α-Amino Acids[J];Journal of Zhejiang University(Natural Science);1989-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved