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《Chemical Research In Chinese Universities》 2002-07
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Studies on Rapid Micro-scale Peptide Mapping Analysis Using a Capillary Micro-reactor

GUO Zhong, ZHANG Qing Chun, LEI Zheng Deng, KONG Liang, MAO Xi Qin, ZOU Han Fa * (National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China)  
Peptide mapping analysis by combining the enzymatic digestion and the MALDI TOF MS has emerged as a powerful tool for identification and characterization of protein. We describe a nanoliter micro reactor prepared with immobilization of enzyme on the porous wall of a capillary. The advantages of the prepared micro reactor for digestion of the proteins are the elimination of enzyme autolysis and concentration of the micro sample. Horse heart cytochrome c is selected as the target protein for the evaluation of micro reactor, and it is found that about 0 1 picomole of protein analyte could routinely be digested for peptide mapping analysis and even femtomole of protein can be well characterized.
【Fund】: 国家杰出青年基金 (批准号 :2 972 5 5 12 )
【CateGory Index】: O657.63
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