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《Chemical Research In Chinese Universities》 2003-05
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Studies on PVDF Hollow Fiber Affinity Membrane for Separation of X-Globulin from Human Plasma( Ⅰ ) -Preparation of PVDF Hollow Fiber Affinity Membrane and Its Adsorption Properties

YU Ji, CAN Hong-Yu, HE Yi, YUAN Jim, CHEN Huan-Lin (College of Materials Science and Chemistry Engineering, ZheJiang University, Hangzhou 310027, China)  
Poly(vinylidene fluoride) (PVDF) hollow fiber membrane used as the matrix was hydrophile-modified by alkali and hydroxyethyl-cellulose(HEC) treatment in this paper. After being activated and coupled, the PVDF hollow fiber affinity membranes with phenylalanine as the ligand were prepared. XPS analysis was used for measuring the changes of chemical structure on the membrane surface. The results indicated that on the membrane surface oxygen and nitrogen contents increased from 5. 6% to 16. 5% and 0. 6% to 3. 0% respectively, while fluorine content decreased from 38. 3% to 23. 0%. The affinity membrane was used for separating γ-globulin (HGG) from human plasma. The result showed that adsorption capacity of HGG by the affinity membrane was 5. 85 mg/g membrane under the condition of pH = 7. 4, I=0. 18(ionic strength) , ρ-1. 0 mg/mL(feed liquid) and the purity of HGG extracted from human plasma was up to 83. 9%.
【Fund】: 国家自然科学基金(批准号:29676038);; 中国博士后科学基金会科研基金
【CateGory Index】: O647
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