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《Chemical Research In Chinese Universities》 2003-09
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Direct Electrochemistry of Hemoglobin Immobilized on Carbon Paste Electrode by Inorganic Film

WANG Quan-Lin, YANG Bao-Jun, LU Gong-Xuan (State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China)  
Direct electrochemical behavior of hemoglobin (Hb) immobilized on carbon paste electrodes by Sol-gel film derived from tetraethylorthosilicate was investigated for the first time. Hb-Sol-gel-modified electrodes showed a pair of well-defined and nearly reversible cyclic voltammetric peaks for HbFe(Ⅲ )/ Fe( Ⅱ ) redox couple at about -0. 275 V(vs. Ag/AgCl) in pH 7. 0 phosphate buffers. The formal potential of Hb heme Fe( Ⅲ )/Fe( Ⅱ ) couple shifted linearly between pH 5. 0-11. 0 with a slope of 51. 85 mV/ pH, suggesting that one proton was coupled to each electron transfer in the electrochemical reaction. It was demonstrated by FTIR spectra that the structure of Hb was still maintained native-like in the film. The immobilized Hb displayed the features of a peroxidase and gave an excellent electrocatalytic response to the reduction of H2O2. Consequently, this work provides a possible route of using Hb-Sol-gel-modified electrodes as the third generation biosensors.
【Fund】: 国家“九七三”计划(批准号:G20000264)资助
【CateGory Index】: O646.54
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