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《Chemical Research In Chinese Universities》 2004-02
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Optimization Method of Multi-segment Gradient for Separation of Ginsenosides in Reversed Phase-High Performance Liquid Chromatography

MA Xiao-Qiong, WANG Long-Xing, XU Qing, ZHANG Feng, XIAO Hong-Bin, LIANG Xin-Miao * (Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China)  
A multi-segment gradient method for the separation of ginsenosides in reversed phase-high performance liquid chromatography(RP-HPLC) was developed. The retention parameters of 8 ginsenosides(Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2 and Rd) were obtained on an ODS2 C18 column(4.6 mm×250 mm) from a series of isocratic acetonitrile-water elution. It is found that two-parameter retention equation of ginsenosides can′t be used to accurately predict the resolution, but the three-parameter retention equation can. The reason is that the retention factor k′ decreases fast while the volume fractions of acetonitrile increase in mobile phase, which make the precision of two-parameter retention equation can′t satisfy the predicting need of resolution. Based on the three-parameter retention equation, an optimum elution program consisting of three segments was developed. Under the optimized gradient conditions, the retention time and resolution of ginsenosides are in good agreement with the predicted ones. The analysis time of ginsenosides is shortened and the resolution is improved by the method.
【Fund】： 国家自然科学基金重点项目 (批准号 :2 0 2 3 5 0 2 0 );; “九七三”方剂关键科学问题的研究项目 (批准号 :G19990 5 44 )资助
【CateGory Index】： O657.7
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【References】
Chinese Journal Full-text Database 8 Hits
1 ZHAO Jing1, PANG Qi-chang2, MA Ji3*, LIU Chuan-ming3, WANG Lin2, CUI Dai-jun2 1. College of Science, South China Agricultural University, Guangzhou 510640, China 2. Key Laboratory of Optoelectronic Information and Sensing Technologies, Jinan University, Guangzhou 510630, China 3. College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China;In Vivo Identification of Radix Panacis Quinquefolii by Spectral Imaging Technology[J];Spectroscopy and Spectral Analysis;2011-01
2 DING Ling,DONG Jun,XIAO Yuan-Sheng,ZHANG Xiu-Li,XUE Xing-Ya,LIANG Xin-Miao(Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China);Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;2013-02
3 GAO Leng,BAO Lei,WANG Gu-yuan,GONG Dian-peng (School of Chemistry and Life Science,Changchun University of Technology,Changchun 130012,China);Ultrasound extraction process of Herba epimedii[J];Chemical Engineer;2010-02
4 ZHANG Min*,ZHAO Suo-qi,ZHAO Guang-lei(State Key Laboratory of Heavy Oil Processing,China University of Petroleum,Beijing 102249,China);Study on the Technique of Removing Organochlorine Pesticide Residues from Ginseng Extract by Sub-critical Propane Extraction[J];Chinese Journal of Pesticide Science;2008-01
5 CHEN Jun-hui,XIE Ming-yong,WANG Hui-qin,PENG Ri-huang,WANG Yuan-xing(Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047,China);Determination of Ginsenosides in Panax quinquefolium. L by HPLC and Study on Their Fingerprints Analysis[J];Food Science;2005-11
6 ZHOU Wei, LUO Zhenshi, ZHOU Pei (Department of Biosynthetic Drug, School of Pharmacy, Fudan University, Shanghai 200032, China);Determination of Ginsenoside Compound-K by Reversed-Phase High Performance Liquid Chromatography[J];Chinese Journal of Chromatography;2005-03
7 XU Qing,WANG Longxing,ZHANG Xi,JIN Gaowa,XIAO Hongbin,LIANG Xinmiao(Dalian Institute of Chemical Physics,The Chinese Academy of Sciences,Dalian 116023,China);Quick Optimization of High Performance Liquid Chromatographic Conditions for Separation of Flavones of Astragalus[J];Chinese Journal of Chromatography;2005-06
8 Jiang Haiping1,Dou Deqiang1,Jing Shuqin2,Liu Fengyun2(1.College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian Liaoning 116620,China;2.Benxi Institute For Drug Control,Benxi Liaoning,117000,China);Study on Ginsenoside and Fingerprint of Linxiashanshen by HPLC[J];Modern Chinese Medicine;2008-04
China Proceedings of conference Full-text Database 1 Hits
1 JIANG Hai-ping~1 DOU De-qiang~1 JING Shu-qin~2 LIU Feng-yun~2 1.College of Pharmacy,Liaoning University of Traditional Chinese Medicine,77 Life One Road DD port,Dalian 116620; 2.Benxi Institute For Drug Control;Study on ginsenoside and fingerprint of linxiashanshen by HPLC[A];[C];2007
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Shan Yichu, Zhao Ruihuan, Zhang Weibing, Liang Zhen, Zhang Yukui * (Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011);Fast Optimization Method of Binary Multi-segment Gradient Elution Conditions for the Separation of Complex Samples in Reversed Phase-High Performance Liquid Chromatography[J];Chinese Journal of Analytieal Chemistry;2002-12
2 ZouHanfa,Zhang Yukui,Bao Miansheng,Dong Lifu and Lu Peichang,Dalian Chromatographic R. & D. Center of China, Dalian Institute of Chemical Physics,Academia Sinica;The Optimization of Binary Mobile Phase Composition in Multi-Step Linear Gradient Elution[J];Chinese Journal of Chromatography;1988-04
3 CHEN Wei, HU Guang lin, WANG Yi ru, WANG Xiao ru (The Key Laboratory of Modern Analytical Science of Ministry of Education and Department of Chemistry, Xiamen University, Xiamen 361005, China);Determination of Six Ginsenosides in Panax Species by High Performance Liquid Chromatography[J];Chinese Journal of Chromatography;2000-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Wei et al(School of Chemical Engineering,University of Science and Technology,Anshan,Liaoning 114051);Study Progress on the Extraction Method of Ginsenosides and Ginsenoside Rb_1[J];Journal of Anhui Agricultural Sciences;2010-29
2 DU Zeng-kai,CHENG Zhou,YANG Xiao-ling,WAN Shu-wen,ZHANG Wen-ju (School of Life Science and Technology ,Tongji University, Shanghai 200092,China;Shanghai Lei-yun-shang Medicine Co. Ltd. , Shanghai 200002,China;Institute of Biodiversity Science ,School of Life Science ,Fudan University, Chinese Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering,Shanghai 200433,China);Comparative Anatomical Study of the Roots of Transplanted Panax ginseng Plants Growing for Different Times[J];Acta Botanica Boreali-Occidentalia Sinica;2006-04
3 Jiao Qingcai; Liu Qian(Department of Biological Science and Technology, Nanjing University . Nanjing 210093)Chen Yaozu(Department of Chemistry, Lanzhou University, Lanzhou 730000);Computer Prediction of Gradient Elution Times in High Performance Liquid Chromatography[J];Chinese Journal of Analytieal Chemistry;1996-08
4 WANG Yu-Tang,LI Xu-Wen,JIN Hai-Yan,YU Yong,YOU Jing-Yan,ZHANG Kun,DING Lan,ZHANG Han-Qi*(College of Chemistry,Jilin University,Changchun 130012,China);Degradation of Ginsenosides in Root of Panax ginseng C.A.Mey. by High-pressure Microwave-assisted Extraction[J];Chemical Journal of Chinese Universities;2007-12
5 Ke Dazhi;Wang Hongning;Chen Dilong;Gu Hengwei;Wang Yaping;Wang Jianwei;Long Xuan;Li Chunli;Luo Chunyan;Department of Geriatric Cardiology,Second Affiliated Hospital,Chongqing Medical University;Department of Histology and Embryology,College of Basic Medical Sciences,Chongqing Medical University;College of Nursing,Chongqing Medical University;College of Life sciences,Chongqing Medical University;Chongqing School of Nursing;;Molecular mechanism of ginsenoside Rg1 in inhibiting proliferation and inducing differentiation in chronic myelocytic leukemia K562 cells[J];Journal of Third Military Medical University;2014-02
6 Li Guangping Zhu Xiaohong Huang Kewu Hu Wanhua(Chemistry and Chemical Engineering Department. Beijing Instituteof Clothing Technology)Li Rongchun (Research Center for Eco-Environmental Sciences, Academia Sinica);APPLICATION OF COMPUTER IN SETTING PROGRAM OF HPLC GRADIENT ELUTION[J];Environmental Chemistry;1990-04
7 WEI YuXia, WANG Lin, XIAO ShengYuan, QING Hong, ZHU Yong, HU GaoFei & DENG YuLin School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China;Peak compression technique in high-performance liquid chromatography[J];科学通报(英文版);2009-16
8 ZHANG Wei;SUN Cheng-he;WANG Shao-yan;LI Qian;WANG Ying-ping;School of Chemical Engineering,Liaoning University of Science and Technology;Engineering Technology Center of Fine Separation of Liaoning Provincial Education Department;Institute of Special Animal and Plant Sciences of CAAS;;Study of Adsorption and Preparation of Ginsenoside Re Molecularly Imprinted Monolithic Column[J];Fine Chemicals;2015-10
9 CHEN Jun-hui,XIE Ming-yong,WANG Hui-qin,PENG Ri-huang,WANG Yuan-xing(Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047,China);Determination of Ginsenosides in Panax quinquefolium. L by HPLC and Study on Their Fingerprints Analysis[J];Food Science;2005-11
10 MAO Qing-li1,2,SHI Zhao-peng1,LI Ling3,LIU Zhong-hua1,ZHU Qi1 (1.College of Horticulture, Hunan Agricultural University, Changsha 410128, China; 2.College of Life Science, Xiaogan University, Xiaogan 432100, China; 3.College of Life Science, South China Normal University, Guangzhou 510631, China);Study on A Rapid Determination of Total Momordicoside by Ultrasonic Extraction from Bitter Melon(Momordica charantia)[J];Food Science;2006-12
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhang Shuxiang Li Nin (Department of Biology Anhui University) (Anhui Food and Brewing Industrial Company) Shi Jun Chang Renhei Wang Lide (Anhui Salt and Vegetable Company) (Department of Biology Anhui University);Preliminary Study New Method of Extraction Panaquilon from P.Qumquefolius L.Fibrous Root[J];JOURNAL OF ANHUI UNIVERSITY(NATURAL SCIENCES);1999-01
2 YOU Li sha,\ ZHANG Wen min (Department of Chemistry, Anhui Normal University, 241000,Wuhu, Anhui, China);MICROWAVE SYNTHESIS OF MONODISPERSE POLYSTYRENE MICROSPHERES WITH LARGE SIZE[J];JOURNAL OF ANHUI NORMAL UNIVERSITY(NATURAL SCIENCE);2000-01
3 GUI Shuang-ying,ZHOU Ya-qiu (Anhui College of Traditional Chinese Medicine,Anhui Hefei 230038,China);Determination of Ginsenosides in Panax Ginseng by Colorimetric Method[J];Journal of Anhui Traditional Chinese Medical College;2003-04
4 SONG Chao 1,2 , ZHUANG Ping 1,2 , ZHANG Long-Zhen1, LIU Jian4,LUO Gang 1,2 1.Key and Open Laboratory of Marine and Estuary Fisheries, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China2.Aquaculture Division, E-institute of Shanghai Universities, College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China3. Superintendency Department of Shanghai Yangtze Estuarine Natural Reserve for Chinese Sturgeon, Shanghai 200002, China;Comparison of nutritive components in muscles between wild and farmed juveniles of Chinese sturgeon Acipenser sinensis[J];Acta Zoologica Sinica;2007-03
5 Wang Miqu,Yan Shilin,Li Weihong,et al Chengdu University of TCM,Chengdu(610075);A Study on Chinese Herb of Cold and Hot on SD Rats[J];Journal of Zhejiang College of Tcm;2002-06
6 Wang Xin,Hong Min, Jin Yan,Mai Yinqiao(Department of Biochemistry);STUDY ON REGULATING EFFECTS OF GINSENOSIDES ON MOLECULAR ENDOCRINE FUNCTION OF PITUITARY-GONADAL SYSTEM IN RATS[J];;1993-01
7 An Gang; Zhao Chunyan; Zhong Guogan(Dept. of Thysiology);Effects of CPPQ on Ca~(2＋)─activated K~＋Channels of mouse T─lymphocytes[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1997-05
8 Zhao Ping,Wang Xiaoming,Sun Chengwen,Jiang Yan,Zhong Guogan,Zhao Chunyan(Dept.of physiol.,Cen.Lab.of physiol.);Influence of oleanolic acid type ginsenoside Ro on single calcium channel activity and free radical ontent of myocardiocyte[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1994-06
9 Zhu Wei,Zhang Hongjun * ,Du Bairong ,et al (Dept.of Immunology; * Dept.of Organic Chemistry);Stimulating effect of PPQ on spleen lymphocyte of mice[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1997-03
10 Zeng Qinghua,Zhan Shu ,Zhang Wenjie,et al (Dept.of physiology The School of Basic Medical Sciences);Study of ginsenoside monomer Rb 1 on whole cell ICa 2+ blockade action in ventricular myocytes of guinea pig[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1997-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 DU Zeng-kai,CHENG Zhou,YANG Xiao-ling,WAN Shu-wen,ZHANG Wen-ju (School of Life Science and Technology ,Tongji University, Shanghai 200092,China;Shanghai Lei-yun-shang Medicine Co. Ltd. , Shanghai 200002,China;Institute of Biodiversity Science ,School of Life Science ,Fudan University, Chinese Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering,Shanghai 200433,China);Comparative Anatomical Study of the Roots of Transplanted Panax ginseng Plants Growing for Different Times[J];Acta Botanica Boreali-Occidentalia Sinica;2006-04
2 Xia Chen,Song Yan,Cao Yang,Zeng Xiaodan,Zhang Yingjiao(Institute of Agro-food Science and Technology,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China);Research on Ultrasound-assisted Extraction of Ginseng Polysaccharides from Ginseng Residue[J];Guangdong Chemical Industry;2012-01
3 DING Ling,DONG Jun,XIAO Yuan-Sheng,ZHANG Xiu-Li,XUE Xing-Ya,LIANG Xin-Miao(Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China);Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;2013-02
4 PENG Yu-Meng;HUO Guang-Hua;HAN Qi-Can;ZHAN Wu-Gen;YE Ya-Jian;WANG Chun-Rong;Jiangxi Agricultural University,Key Laboratory of Protection and Utilization of Biological Resources in Nanchang;;Separation of Saponin Analogue from Schima Superba with Activity Resisting Magnaporthe Oryzae[J];Chinese Journal of Analytical Chemistry;2014-01
5 LIU Hua;ZHAO Xin;QI Tian;QI Yun-peng;FAN Guo-rong;Department of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University;Department of Pharmacognosy,School of Pharmacy,Anhui University of Traditional Chinese Medicine;;Establishment of the Model for Online Monitoring of the Column Separation and Purification Process by Near-Infrared Spectroscopy and Determination of Total Ginsenosides in Folium Ginseng[J];Spectroscopy and Spectral Analysis;2013-12
6 Yu Hongliang;Chang Mingxiu;Cao Henghai;Bo Liwei;Yang Yongjun;Li Chongmin;Li Ming;Henan Province Research Institute for Population and Family Planning;;The lethal dose test of Ginsenoside CK and Icraiin for sperm in vitro[J];Engineering Sciences;2015-06
7 Wang Fenghua,Zhou Ying,Deng Xiaobin,Tian Shen,Zhang Ting(Affiliated Hospital of Prevention and Treatment Institute of Occupational Disease of Act of Labour Health in Hunan,Changsha 410007);Determination of Ginsenoside Rb1 in Shenziling Capsule by HPLC[J];Guiding Journal of Traditional Chinese Medicine and Pharmacy;2010-11
8 ;Research progress on ginseng in forest[J];Journal of Jilin Medical College;2011-02
9 YIN Chun-mei1,LI Xiu-chang2,SUN Jia-ming2,TANG Shan2,CHEN Xiang-tao2,Zhang Hui2 1.College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China;2.Development Center of Traditional Chinese Medicine and Bioengineering,Changchun University of Chinese Medicine,Changchun 130117,China;Cluster Analysis of Content of Total Saponin in Panax quinquefolium[J];Journal of Jilin Agricultural University;2010-03
10 LIU Xiao-dong1,YAN Ming2,HUO Shi-xia1,ZHANG Lan-lan1(1.Pharmacy College of Shihezi University,Shihezi 832003,Xinjiang,China;2.Xinjiang Institute of Traditional Uighur Medicine,Urumqi 830049,Xinjiang,China);High performance liquid chromatography applied to the comparative study of the fingerprints for different extracts of Vernonia anthelmintica(L.) Willd[J];Journal of Medical Postgraduates;2009-08
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 SHAN Yi chu, ZHAO Rui huan, ZHANG Wei bing, ZHANG Yu kui (National Chromatographic R & A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China);A New Method for Predicting Retention Time of Solutes Under Linear Gradient Elution Conditions in RP-HPLC[J];Chinese Journal of Chromatography;2001-03
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