Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chemical Research In Chinese Universities》 2004-03
Add to Favorite Get Latest Update

Preparation and Structural Characterization of a New Corn Anti-oxidative Peptide

XU Li 1, LI Xiang Lu 1, WU Xiao Xia 1, WANG Hua 2, HOU Rui Zhen 3, HUANG Yi Bing 1, ZHANG Xue Zhong 1* (1. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130023, China; 2. China Japan Union Hospital of Jilin University, Changchun 130033, China; 3. College of Chemical Engineering, Changchun University of Technology. Changchun 130012, China)  
A kind of new corn anti oxidative peptide was prepared via protease hydrolysis of zein catalyzed by alcalase, an alkaline protease under a certain condition, followed by isolation and purification. The hydrolysis time was fixed on the basis of the dependence of antioxidative activity on hydrolysis degree. It was found that the hydrolysis for 4 h gave the highest antioxidative activity. The isolation and purification steps include ultrafiltration with a membrane of cutoff M w 1 000, successive chromatography on Sephadex G 15, Amberlite CG 120(Na) ion exchange, Sephadex G 10 and preparative HPLC columns. The amino acid sequence of the antioxidative peptide was characterized to be Leu Asp Tyr Glu by amino acid analysis and electrospray ion trap mass spectrometry. We also discussed the possible anti oxidative mechanism of the antioxidative peptide on the basis of its structure.
【Fund】: 国家“十五”科技攻关计划 (批准号 :2 0 0 1BA960 C)资助
【CateGory Index】: O629
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Qiang,ZHOU Zheng-yi,SUN Yu-jun,XU Ting(Life Science College,Anhui Science and Technology University,Fengyang 233100,China);Study on Separation and Purification of Rice Bran Antioxidant Peptide[J];Journal of Anhui Science and Technology University;2008-01
2 Mu Xuejiao,Zhang Qiang(Anhui Science and Technology University,college of life science,Fengyang Anhui233100);Progress in Research on Antioxidant Bioactive Peptides from Plant[J];Forest By-Product and Speciality in China;2008-03
3 ZHU Yun-han1,LIU Rui1,2,WU Hao1,2,WANG Ling-chong1,2(1School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210029,China;2Jiangsu Research and Development Center of Marine Drugs,Nanjing 210029,China);Progress of structure-activity relationship of bioactive peptides[J];China Journal of Traditional Chinese Medicine and Pharmacy;2012-10
4 PANG Cai-xia 1,JIN Ying-zi 2(1 The HUHhot Vocational College HUHhot 010051 China;2 The Xin-Jiang Light Industrial Vocational Technologyical College Wulumuqi 830021 China);The Application of Zein[J];Journal of Liaoning Agricultural Vocation-technical College;2005-01
5 WANG Xiao-yan,TANG Chuan-he(College of Light Industry and Food Science,South China University and Technology,Guangzhou510640,China);Preparation of antioxidant peptide from soy protein and its structure-activity relationship[J];Cereals & Oils;2009-04
6 Wang Guanyu,Chen Ye,Du Yue(College of Food Engineering and Biology Technology,Tianjin University of Technology,Tianjin 300457,China);Advance Study on Corn Gluten Meal[J];Academic Periodical of Farm Products Processing;2008-07
7 ZENG Ming-yong,GUO Yao,LIU Zun-ying,DONG Shi-yuan,ZHAO Yuan-hui,CUI Wen-xuan (College of Food Science,Ocean University of China,Qingdao 266003, China);Preparation of radical scavenging peptides from Oreochromis niloticus skin gelatin and its antioxidative activity[J];Journal of Fisheries of China;2008-01
8 Wei Linjing,Li Daguang,Shu Xugang;Synthesis method of glutamine dipeptide and its application in pig breeding[J];China Feed;2012-05
9 Wu Hui,Li Li-li,Li Xiao-feng,Yu Yi-gang(College of Light Industry and Food Science,South China University of Technology,Guangzhou 510640,China);Study on Preparation of Polypeptide from Corn Gluten Protein by Immobilized Enzyme Hydrolysis in Nonaqueous Media[J];Food and Fermentation Industries;2010-07
10 Wu Hui,Li Li-li,Li Xiao-feng,Yu Yi-gang (College of Light Industry and Food Science,South China University of Technology,Guangzhou 510640,China);Preparation of Corn Peptide in Nonaqueous Media and Its Antialcoholism Activity in vitro[J];Food and Fermentation Industries;2010-12
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XIA Hai-jun(School of Resources and Environment,Anhui Agricultural University,Hefei,Anhui 230036);Research Progress in Bamboo Leaf Extracts Bioactivity[J];Journal of Anhui Agricultural Sciences;2009-28
2 LI Meng-qing, WANG Ping, LIU Gui-min(Department of Chemistry, Hebei University of Technology, Tianjin300130,China);Measurement of superoxide dismutase-like activity of proanthocyanidins[J];China Surfactant Detergent & Cosmetics;2005-01
3 CHEN Tianfei WU Dayang LI Chunfeng (Sericulture and Biotechnology College of Southwest Agricultural University, The Key Sericultural Laboratory of Agricultural Ministry, Chongqing 400716, China);ANALYSIS OF SOD ACTIVITY IN CRYOPRESERVED SPERMS OF SILKWORM[J];Newsletter of Sericultural Science;2004-04
4 Jiang Heyuan Lu Feijie Tai Jianxiang (Institute of Crop Breeding and Cultivation ,CAAS,Beijing,100081);BIOACTIVE COMPONENTS OF SOYBEAN AND THEIR FUNCTION[J];SOYBEAN SCIENCE;2000-02
5 LIU Bo,LU Ming-chun,LIU Ying-xin,LI Zhi-rui,JIN Feng-xieSchool of Biological & Food Engineering,Dalian Polytechnic University,Dalian,116034,China;Anti-oxidative activity of zein hydrolyte by protease from Bacillus natto[J];Journal of Dalian Polytechnic University;2008-02
6 LI Fei-Fei WU Yong-Jun QU Ling-Bo WU Yi-Ming (Department of Chemistry,Zhengzhou University,Zhengzhou 450052,P. R. China) (College of Public Health,Zhengzhou University,Zhengzhou 450052,P. R. China);Study on Anti-Radical Activity of Chinese Medicine Morinda Officinal is How[J];Chinese Journal of Spectroscopy Laboratory;2005-03
7 Fu Gang,Li Cheng,Ma Chang-zhong,Li Xi (Food department,Sichuan agricultural university,Yaan 625014,China);Investigation of Swine Bone Antioxidant Peptide Preparation by Enzymatic Hydrolysis[J];Modern Food Science and Technology;2006-03
8 CHENG Hua;LI Lin-ling;WANG Shao-bin;XU Feng;WANG Yan;CHENG Shui-yuan;Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratories;College of Chemistry and Life Science,Huanggang Normal University;Technology Education Department,Forestry Department of Hubei Province;;The Relationship Between Flower Bud Differentiation and the Changes of Metabolism-related Enzyme Activity in Castanea mollissima‘Bayuehong'[J];Northern Horticulture;2013-20
9 XIANG Tian-yong 1, ZHANG Chi 2, XIE Da-ping 1 (1. Hunan Agricultural University, Changsha 410128, China; 2.Hubei Institute for Nationalities,Enshi 445000,China);The Separation and Purification of the Bioactive Substances of Indocalamus Leaves[J];Journal of Hubei Institute For Nationalities;2002-03
10 FENG Xiao-mei,HAN Yu-qian,ZHAO Zhi-qiang,JIANG Xin,JIANG Shou-lei(National Enginering Research Center For Marine Drugs,Ocean University of China,Qingdao 266101,China);Preliminary study on the preparation and physicochemical characteristics of bioactive peptides from Crassostrea gigas thunberg[J];Chinese Journal of Marine Drugs;2006-02
China Proceedings of conference Full-text Database 1 Hits
1 ;Advances in Studies on Chemical Components and Biological Activities of Fig (Ficus carica L.)[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Liu Xiaoging (Deprtment of Biology)Yao Hongyi(the Biological Institute of Anhui Province)Zhang Yuanguan(Hefei Pharmaceutid Factony);Extraction Technology and Property Researches of Leaf Protein[J];JOURNAL OF ANHUI UNIVERSITY(NATURAL SCIENCES);1994-03
2 Ding Yi (Experiment and Tech Center, Anhui Univ) Liu Xiaoying Ge Honghua Gan Dingjuan (The Biological Institute, Anhui Univ);Studies on the Decolorization of Leaf Protein Concertrates and the Properities of Pigment[J];JOURNAL OF ANHUI UNIVERSITY(NATURAL SCIENCES);1998-01
3 WANG Xue-zhi, REN Gui-jiu, WU Zhi-chuan, XIE Tong-yun, GENG Chuan, DING Sheng-li (Department of Biochemical Engineering, Anhui Institute of Mechanical and Electrical Engineering, Anhui Wuhu 241000, China);Initial results of research to produce corn protein-based biodegradable plastic[J];JOURNAL OF ANHUI INSTITUTE OF MECHANICAL AND ELECTRICAL ENGINEERING;2000-01
4 XUE Zheng-lian, LIU Yan, YANG Hao (Dept. of Bioch.Engn., Anhui University of Technology and Science, Wuhu 241000, China);Study on the plant leaf protein[J];Journal of Anhui University of Technology and Science;2005-02
5 Song Zong-qing1,Li Chun-bao1,Kan Qing-hua2,Tang Bin2(1.Qiaqia Food Co.,Ltd.,Hefei 230601,China;2.Modern Analysis Center,Anhui Polytechnic University,Wuhu 241000,China);Research on the fat-soluble flavor compounds in melon seeds of baked ank raw sunflower seeds[J];Journal of Anhui Polytechnic University;2011-01
6 Jing Qingdong(Zhengzhou college of Aminal Husbandry Engineering,Department of verinery medicinezhengzhou 45008);Adrance in Research of Carnosine Antioxidizability[J];Anhui Agricultural Science Bulletin;2007-02
7 Zhang Zhong,Li Jing,Li Zheng-tao,Gong Fa-yong*(Xichang College,Xichang,Sichuan 615013);Study on the Conditions of Extracting Polysaccharides in Tea Seed by Means of Ultrasonic Assistant Extraction[J];Anhui Agricultural Science Bulletin;2007-10
8 LI Na (College of Science,Liaoning Technical University,Fuxin,Liaoning 123000);Research progress in nutritional ingredients and biological activities of pine pollen[J];Anhui Agricultural Science Bulletin;2008-13
9 LI Feng et al(Biotechnology and Science College,Shenyang Agricultural University,Shenyang,Liaoning 110161);Study on Elimination Protein from the Roofs of Aralia continentalis Kitag Polysaccharide[J];Journal of Anhui Agricultural Sciences;2006-01
10 WANG Ying et al (College of Life Sciences,Linyi Normal University,linyi,Shandong 276000);Progress in Research on solid Phase Synthesis Polypeptide[J];Journal of Anhui Agricultural Sciences;2006-22
China Proceedings of conference Full-text Database 4 Hits
1 ZHU Shu-wen, CHEN Li-hua, AI Xiao-jie, HUA Xiu-guo ,LI Hua-wei, WEI Xiang-mei School of Agriculture and Biology, Shanghai JiaoTong University, Shanghai 201101 Shanghai Zoological park, Shanghai 200335,China;Concentration of reproduction hormone in the peripheral serum of Huangshan macaque during the oestrous cycle[A];[C];2005
2 DONG Yu-lan CHEN Yao-xing WANG Zi-xu BAI Yong-ping XIE Dian ZHAO Jiao-hui (College of Animal Medicine,China Agricultural University,Beijing 100094,China);Effect of Sympathetic Nerve on Early Embryonic Development and Serum Sex Steroid of Mice[A];[C];2008
3 Ayimuguli~(1,2) CAI Yong~3 FAN Guang-Li~2 (1.Life Science and Engineering College of Northwest University for nationalities,Lanzhou 730030; 2.College of Veterinary Medicine;Northwest Science and Technology University of Agriculture and Forestry;Yangling 712100; 3.Experiment Center of Northwest University for nationalities,Lanzhou 730030 China);Effects of Estrogen on Bcl-2 and Bax in Cerebral Cortexes and Hippocampus of Rat[A];[C];2008
4 Zhou Bin, Wu Yujuan (Institute of Bee Products, Ymgzhou University,225009);Studies on the Effects of Pollen on the Caste Differentiation in the Honeybee[A];[C];2004
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WANG Jin-heng et al(Center of Research and Development,Dancheng Caixin Sugar Co.,Ltd.,Dancheng,Henan 477150);Research on the Extraction of Pigment and Gliadin in Corn[J];Journal of Anhui Agricultural Sciences;2009-34
2 ZHANG Hong-li et al (Guangdong Food and Drug Vocational College,Guangzhou,Guangdong 510520);Research Progress of Antioxidant Peptide[J];Journal of Anhui Agricultural Sciences;2011-02
3 ZHOU Yong et al(Department of Public Health,Jilin Medical College,Jilin,Jilin 132013);Study on Antioxidant Activity of Active Peptide from Skin of Forest Frog(Rana chensinensis)[J];Journal of Anhui Agricultural Sciences;2012-14
4 Mu Xuejiao,Zhang Qiang(Anhui Science and Technology University,college of life science,Fengyang Anhui233100);Progress in Research on Antioxidant Bioactive Peptides from Plant[J];Forest By-Product and Speciality in China;2008-03
5 WANG Xin1,JIANG Lianzhou1,2,WU Xia1,TIAN Ruihong1(1.Food College,Northeast Agricultural University,Harbin 150030,China;2.National Institute of Soybean Engineering Technology,Harbin 150030,China);Study on alkaline protease hydrolysis of soy protein isolate and its antioxidant activity[J];Journal of Northeast Agricultural University;2011-11
6 DONG Jiang-tao,ZHOU Ting-ting,LI Yan,SONG Fei(College of Food Science & Technology,Shanghai Ocean University,Shanghai 201306,China);Purification of Soybean Peptides with Anti-oxidant Activity[J];Soybean Science;2010-04
7 LUO Li-hua,SUN Jian-hua,HUANG Qiu-xia,WU Shan-guang, LAN Xiong-diao,LIAO Dan-kui(College of Chemistry and Chemical Engineering,Guangxi University;Guangxi Key Lab of Perochemical Resource Processing & Process Intensification Technology,Nanning 530004,China);Optimization of enzymatic hydrolysis conditions parameters for antioxidant capacity of hydrolysates from protein of Tilapia heels[J];Journal of Guangxi University(Natural Science Edition);2011-03
8 ZENG Xiao-fang1,LU Chang-hai1,CHEN Yao2,BAI Wei-dong1(1.College of Light Industry and Food,Zhongkai University of Agricultural and Engineering,Guangzhou 510225,China)(2.Group of Huang shanghuang,Guangzhou 510170,China);Reviews of Application of Fat Oxidation Control Technology in the Cantonese Sausage Production[J];Modern Food Science and Technology;2012-08
9 ZHU Yun-han1,LIU Rui1,2,WU Hao1,2,WANG Ling-chong1,2(1School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210029,China;2Jiangsu Research and Development Center of Marine Drugs,Nanjing 210029,China);Progress of structure-activity relationship of bioactive peptides[J];China Journal of Traditional Chinese Medicine and Pharmacy;2012-10
10 XU Jun-jie1,YU Hong-quan2,ZHAO Wei1,JIN Hong3,WEN Na3,QI Ling3,LIU Xing-ji2(1.Department of Biochemistry,Jilin Medical College Jilin 132013,China;2.Department of Neurosurgery, First Hospital,Jilin University,Changchun 130021,China;3.Department of Pathology,Jilin Medical College,Jilin 132013,China);Induction of broccoli polypeptide on apoptosis of C6 glioma cells[J];Journal of Jilin University(Medicine Edition);2013-01
China Proceedings of conference Full-text Database 1 Hits
1 Wang Being Ma Haile (School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013);Enzymatic Preparation of Anti-oxidation Peptide from Potato Pulp[A];[C];2010
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved