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《Chemical Research In Chinese Universities》 2005-02
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Electrochemical Detection of DNA Sequences Using Nano-magnetic Particles

LIU Ai-Li1, PENG Tu-Zhi 1*, CHENG Qiong 1,2(1. Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China; 2. Department of Chemical Engineering, Jiaxing College, Jiaxing 314001, China)  
The carboxyl nanoparticles were prepared by dispersing polymerization method. The oligonucleotides were immobilized on the surface of the nano-magnetic particles by EDC and NHS. The novel DNA probe of magnetic particle can concentrate the trace amount of complementary DNA selectively from the sample solution onto the gold film electrode under a magnetic field. Differential pulse voltammetry was used to detect DNA with an electroactive indicator of neutral red which could bind to double stranded DNA. The linear range for the determination of target DNA is from 1.0×10 -6 to 5.0×10 -9 mol/L, and the detection limit is found to be 8.6×10 -10 mol/L.
【Fund】: 国家自然科学基金 (批准号 :2 9975 0 2 4 2 0 2 75 0 3 4);; 浙江省重点科技计划项目 (批准号 :2 0 0 3 C2 10 2 4)资助
【CateGory Index】: Q52
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