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《Chemical Research In Chinese Universities》 2005-02
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In-situ UV and CD Spectroelectrochemistry Investigation of the Electrochemical Reduction Reaction Inducing Conformational Transition of Microperoxidase-11

JIANG Xiu-E, ZHANG Zhe-Ling, HUANG Wei-Min, LIU Xiao-Qiang, WANG Er-Kang, DONG Shao-Jun*(State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China)  
Electrochemical reduction process of microperoxidase-11 was investigated by in-situ UV-Vis and CD spectroelectrochemistry methods with a long optical path thin layer cell. The spectra obtained were analyzed by singular value decomposition least square and double logarithmic analysis methods. The results showed that the electrochemical reduction induced the conformational transition of MP-11 from random coil to α-helix and provided an important information for understanding biological electron transfer mechanism coupled with conformational transitions.
【Fund】: 国家自然科学基金 (批准号 :2 0 2 75 0 3 6 2 0 2 75 0 3 7)资助
【CateGory Index】: O629
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