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《Chemical Journal of Chinese Universities》 2006-10
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Element Transference Characteristics and Element Speciation Analysis in Panax quinquefolium. L by RP-HPLC-ICP-MS

CHEN Jun-Hui 1,2,XIE Ming-Yong 1*,WANG Feng-Mei 3,WANG Xiao-Ru 3,4,Frank S.C. LEE 4(1.Key Laboratory of Food Science of Ministry of Education,Nanchang University,Nanchang 330047,China;2.Faculty of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266003,China;3.Key Laboratory of Modern Analytical Science of Ministry of Education,Xiamen University,Xiamen 361005,China;4. Qingdao Key Laboratory of Analytical Technology Development and Standardization of Chinese Medicines,First Institute Oceanography of SOA,Qingdao 266061,China)  
Inductively coupled plasma mass spectrometry(ICP-MS)and high performance liquid chromatography coupled with ICP-MS(HPLC-ICP-MS)were applied to the study of the chemical speciation of inorganic elements in Panax quinquefolium.L.Chloroform,water and ethyl alcohol were employed to extract Panax quinquefolium.L,and the extracts were then determined by ICP-MS in order to analyze the transference characteristics of the elements.The RP-HPLC-ICP-MS technique was applied for the analysis of element speciation.The results showed that the polarity of the extraction solvent affects significantly the elemental transference ratio.The transference characteristics of kinds of elements including Mg,Ca,P,Mn,Sr,Mo and Pb were in linearity proportion with the polarity of extraction solvent.The inorganic and organic species of the elements were well separated by HPLC-ICP-MS under the following conditions:C_ 18 bonded silica gel column,0.10 mol/L nitric acid and super pure water as the mobile phase,flow rate of 0.4 mL/min and injection volume of 20 μL.
【Fund】: 国家自然科学基金重点项目(批准号:20235020)资助.
【CateGory Index】: R284
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