Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chemical Journal of Chinese Universities》 2006-11
Add to Favorite Get Latest Update

Detection of Potato Spindle Tuber Viroid Using RNA Hybridized Chips

GU Yü~(1,3),DU Zhi-You~2,LANG Qiu-Lei~2,CHEN Ji-Shuang~2,HE Nong-Yue~3*(1.Department of Biology and Biochemistry,School of Basic Medical Science,Nanjing University of Traditional Medicine,Nanjing 210029,China;2.Laboratory of Microbial Molecular Biology,Zhejiang University,Hangzhou 310029,China;3.State Key Laboratory of Bioelectronics,Southeast University,Nanjing 210096,China)  
Potato diseases caused by potato spindle tuber viroid(PSTVd) bring about substantial losses in agricultural production.So developing a high throughput,rapid,sensitive and specific detection method is ne-cessary. This article reports a new method to detect plant viroidRNA glass slide hybridization.This me-thod combines dot blot hybridization technique with cDNA microarray technique.We could spot a number of RNA samples extracted from new leaves of potatoes onto a silanated glass slide directly and then perform the hybridization on the slide with Cy5-labeled probe of PSTVd prepared by PCR.Analyzing the results of hybri-dization, we could ascertain which sample is infected by PSTVd.The factors affecting the results were optimized and used to test six potato samples with PSTVd-specific probes.The developed method was used to detect two samples which infected by PSTVd.These results were in good accordance with those from the RT-PCR approach and the samples are proved to be PSTVd by cloning and sequencing.So it is an accurate and reliable method for detecting plant viroid and the first report for the diagnosis of PSTVd using dot blot hybridization and RNA glass side hybridization with fluorescently labeled probe.
【Fund】: 江苏省社会公益研究项目(批准号:BM2003701);; 国家自然科学基金(批准号:60571032)资助
【CateGory Index】: S435.32
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved