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《Chemical Journal of Chinese Universities》 2007-01
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Electrochemical Studies on Interaction Between Dinuclear Copper(Ⅱ) Complex Containing Dimethylglyoxime and DNA

XU Gui-Yun~(1,2),JIAO Kui~(2*),LI Yan-Tuan~3,REN Yong~2,ZHANG Xu-Zhi~2(1.College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266003,China;2.College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266042,China;3.Institute of Marine Drug and Food,Ocean University of China,Qingdao 266003,China)  
Cyclic voltammetry was used to study the interaction between dinuclear copper(Ⅱ) complex containing dimethylglyoxime[Cu_2(Hdmg)_4] and DNA.The influences of pH,temperature,ionic strength and concentration of Cu_2(Hdmg)_4 on the interaction were described,and the mechanism of the interaction was also discussed.The result shows that Cu_2(Hdmg)_4 can interact with the bases of DNA to form non-electroactive complex,which can decrease the anodic peak current of Cu_2(Hdmg)_4.The interaction between Cu_2(Hdmg)_4 and ssDNA is stranger than that between Cu_2(Hdmg)_4 and dsDNA because of the exposed bases of ssDNA.The combining ratios of Cu_2(Hdmg)_4 to ssDNA and to dsDNA are 2∶1 and 1∶1,and the combining constants 3.56×10~9 and 2.75×10~5,respectively.
【Fund】: 国家自然科学基金(批准号:20375020)资助
【CateGory Index】: Q523
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