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《Chemical Journal of Chinese Universities》 2007-09
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Bioeffects of Silica Nanoparticles and Silica Microparticles as Carriers for Enzyme Immobilization

SHI Hui1,2,4,HE Xiao-Xiao1,2,3,4,WANG Ke-Min1,2,4,YUAN Yin1,2,4,TAN Wei-Hong1,2,4(1.State Key Laboratory of Chemo/Biosensing and Chemometrics,2.Engineering Center for Biomedicine,3.Institute of Life Science & Biological Technology,4.Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province,Changsha 410082,China)  
In this paper,the differences between silica nanoparticles(SiNPs)and silica microparticles(SiMPs)in the bioeffects of them as carriers for enzyme immobilization were investigated.By choosing bovine liver catalases and horseradish peroxidases as the multimeric enzyme model and monoer enzyme model,respectively,four kinds of immobilized enzymes were obtained through the covalently binding method.After the characterization of above four immobilized enzymes,the following conclusion was stated.Firstly,in the yields of enzyme activity,SiNPs exhibit non-selective excellent biocompatibility to both enzyme models;secondly,SiMPs are superior to SiNPs on thermostability;thirdly,both SiNPs and SiMPs could greatly improve the stability of enzymes in organic solvents;lastly,there is no obvious rule indicated on the optimum temperature of enzyme catalysis,namely,SiNPs are better than SiMPs in the multimeric enzyme model and SiMPs possess much more advantages over SiNPs in the monomer enzyme model.The results would be instructional to the evaluation of nanomaterials' bioeffcts and the application of nanomaterials for enzyme immobilization.
【Fund】: 国家“九七三”计划(批准号:2002CB513110);; 国家高科技发展规划项目(批准号:2003AA302250);; 国家科技攻关计划项目(批准号:2003BA310A16);; 教育部重点科研项目(批准号:107084);; 教育部新世纪优秀人才支持计划(批准号:NCET-06-0697);; 科技部国际合作重点项目(批准号:2003DF000039);; 国家自然科学基金(批准号:90606003 20405005);; 湖南省杰出青年基金(批准号:06JJ10004)资助.
【CateGory Index】: Q814.2
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