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《CHEMICAL RESEARCH IN CHINESE UNIVERSITIES》 1999-04
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Fluorescence Immunoassay for HBsAg Using a Thermal Phase Separating Polymer

ZHU QingZhi\+*, YANG HuangHao, LI DongHui XU JinGou, ZHANG Yi, ZHANG ChangGong (The Research Laboratory of SEDC of Analytical Science for Material and Life Chemistry, Department of Chemistry, Cancer Research Center, Xiamen University, Xiamen, 361005)  
A novel fluorescence immunoassay for HBsAg was developed by using polyNisopropylacrylamide(PNIP) as an immunoreaction carrier. In sandwich immunoassay, after two steps immunoreaction, PNIP precipitates out of water above a critical temperature of 31 , enabling a polymerbound immune complex to be separated from the solution. The calibration graph for HBsAg was linear over the range of 05100 g/mL with a detection limit of \{10 ng/mL\}. This method combines some advantages of both homogeneous and heterogeneous immunoassays, and has been applied to determining the HBsAg in human blood serum with satisfactory results.
【Fund】: 教育部博士点基金 福建省自然科学基金
【CateGory Index】: O657.3
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