Study on Aloe Extract C induced cytotoxic factors in mice
XIE Guang-lin, HE Shen, HE Ming-chang (College of Basic Medical Science, China Medical University, Shenyang 110001 )
Objective: To study the effect of Aloe Extract C ( AE - C) in inducing cytotoxic factors in mice. Methods: Mice were injected with AE - C through abdominal cavity for 5 days, blood was taken for separating serum on the sixth day. Y99 , L929 and A549 tumor cell lines were used as target cells to assay cytotoxic factors activity of the serum. The lasting time of the activity, its sensitivity to temperature and the cytotoxic activity of different concentration of the serum were observed. Results: AE - C could induce the production of cytotoxic factors in BALB/C mice, compared with the control, there was a significant differences ( P 0. 01 ) . Cytotoxic activity reached the peak 24 hours after last injection of AE - C and began to drop 4 days later. The cytotoxic activity disappeared 5 days later. The cytotoxic acitivity was still obvious with 200 times dilution of the serum. Conclusions: AE - C can induce the production of cytotoxic factors in BALB/C mice.