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《Academic Journal of Guangdong College of Pharmacy》 2005-02
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Optimized separation conditions for the determination of encapsulation efficiency of liposome-encapsulated capsaicin

HONG Hui~1,LONG Xiao-ying~(1,2#),LI Li-ren~1,LIN Dan~1,LIANG Shao-hua~1(1.Department of Pharmaceutics, Guangdong College of Pharmacy,Guangzhou 510240,China; 2.Key Laboratory of pharmaceutics of Chinese Materia Media,Nanfang Medical University,Guangzhou 510515,China)  
Objective To optimize the separation conditions for the detection of encapsulation efficiency of liposome-encapsulated capsaicin on Sephadex G50 mini-column. Method The effects of water content of the gel, centrifuge speed and time on the measurement of encapsulation efficiency were investigated using UV absorbance of the eluate as standard. Result When Sephadex G50 was centrifuged at 1 000 r/min for 6 s for removing 3 ml of water, then at 2 000 r/min for 5 min, liposome-encapsulated capsaicin was separated thoroughly from the gel and free capsaicin remained in the column. Conclusion Proper water content of the gel, suitable centrifuge speed and time are helpful for the separation of liposome-encapsulated capsaicin from the free capsaicin on the column.
【Fund】: 广州市科技局科技攻关课题(编号: 2003Z3-E5121)
【CateGory Index】: TQ460.1
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