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《Journal of Guangdong College of Pharmacy》 2007-02
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Delayed effects of TMPZ on neonatal rat myocardiocytes subjected to anoxia/reoxygenation

GU Xiao-ming1,HE Ming2 (1.LongHua Epidemic-Prevention and Healthcare Institute of Baoan District,Shenzhen,Guangdong 5181091,China;2.Department of Pharmacology,Medical College of NanChang University,Nanchang,Jiangxi 330006,China)  
Objective To investigate the delayed myocardioprotection of TMPZ pre-conditioning.Methods Cultured myocardial cells of neonatal SD rats have been randomly divided into 4 groups: Control group;anoxia reoxygenation(A/R)group;anoxia preconditioning group(APC);TMPZ group;In each group,the cardiomyocytes were subjected to anoxia(3 hr) followed by reoxygenation(1 hr) 24 hr after anoxic(or TMPZ) preconditioning on the model of the cultured cardiomyocytes.At the end of experiment,myocardial cells beating rate,cell viability and the activity of LDH in culture were measured.Results ①Beating rate of ventricular myocytes: The beating rate was significantly slow in A/R group(13±3 beats/min) compared with that of control group(P0.05);Between APC group(84±6 beats/min) and TMPZ group(85±3) beats/min,the beating rate was similar to each other(P0.05),and compared with A/R,the beating rate was significantly increased(P0.05);②the cell viability:The cell viability of A/R(36.23±4.05)% was significantly decreased when compared with that of control group(P0.05);the viability of APC(83.76±6.92)%,TMPZ(80.56±4.65)% was significantly increased when compared with A/R respectively,(P0.05),between APC and TMPZ,the cell viability was similar to each other(P0.05);③LDH activity: LDH activity of A/R(36.73±2.35) U/L was significantly increased when compared with Control group(P0.05);LDH activity of APC(5.70±0.63)U/L or TMPZ(5.74±0.34) U/L was significantly decreased when compared with A/R respectively(P0.05),but LDH activity of TMPZ was similar to that of APC group(P0.05).Conclusion TMPZ preconditioning can mimic the delayed protection on cultured neonatal rat myocardiocytes with hypoxia/reperfusion injury.
【CateGory Index】: R285
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