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《Journal of Guangdong Pharmaceutical College》 2011-02
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Myocardial fibrosis of metabolic syndrome rats induced by low dose of STZ and L-NAME with high-fat diet

LIU Xiao-ying1,Dong Hui2,FU Yong-heng1,LIANG Jia-liang1,WANG Yan2,CHEN Da-qiang2,YANG Min1,DENG Chun-yu1,YU Xi-yong1(1.Guangdong Academy of Medical Science,.Medical Centre of Guangdong General Hospital;2.Department of Clinical laboratory,Guangdong General Hospital,Guangzhou,Guangdong 510080,China)  
Objective To establish a rat model of metabolic syndrome and investigate the changes in cardiac function and cardiomyopaphy of the model,and to supply an experimental platform for the therapeutic study.Methods Male SD rats were fed with a high-fat diet for 6 weeks after being administered 20 mg/kg STZ through tail intravenous injection,and were given with 0.04% L-NAME solution mixed with 10% fructose through daily drinking.The change of metabolism syndrome basic characteristics was observed and recorded.Isolated heart function test was employed to determine the cardiac function of the rats.The myocardium pathological section was stained by HE and the expressions of CollagenⅠand Collagen Ⅲ in myocardial tissue were detected by immunofluorescence.Results Blood pressure and plasma triglyceride,high-density lipoprotein and LDL/HDL levels of the treated rats were all increased significantly after 3 weeks(P0.05).The fasting plasma glucose and AUC of oral glucose tolerance test of treated group increased(P0.05) after 6 weeks.The heart rate,LVPP,LVDP,-dp/dt max and Vpm of the treated rats all decreased but LVEDP increased significantly(P0.05).By staining,broken junctions and wide gaps between muscle fibers were observed in the myocardium of the treated rats,expression of Collagen Ⅲ increased significantly while expression of Collagen I didn't change obviously.Conclusion Myocardial fibrosis,cardiac dysfunction and other typical changes of metabolic syndrome with high blood pressure,high glucose and lipid could be induced in treated rats with high-fat diet,which could be applied as an ideal animal model for the study of cardiac diseases in MS.
【Fund】: 国家973项目(2005CB523305);; 广东省科技计划项目(2009B080701054);; 广东省中医药管理局科研基金(2010239)
【CateGory Index】: R589
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 Cao Tingbing, Yan Zhencheng, Shen Chengyi et al. Department of Hypertension and Endocrinology, Daping Hospital, Third Military Medical University, Chongqing 400042, China;Reproduction of a rat model of metabolic syndrome to study the related gene expression[J];Medical Journal of Chinese People's Liberation Army;2005-08
2 SU Chang-hai,FU Ji-hua,SHAN Ying,KONG Shu-jia,WANG YuanDepartment of Physiology,China Pharmaceutical University,Nanjing 210009,China;A rat metabolic syndrome model induced by stress accompanying high-glucose-fat-diet[J];Journal of China Pharmaceutical University;2006-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 JIN Qi-guan,ZHANG Mei-ling,PAN Yan-juan,LUO Qiang,CHEN Bao,DENG Yu-qiang(Physical education college,Yangzhou University,Yangzhou 225009,Jiangsu China);Intervention Effects of Aerobic Exercise and Soy Protein Hydrolyzate on the Informing of Insulin Resistance and Blood Lipid Metabolism in SD Rats Fed High Fat Diet[J];Journal of Beijing Sport University;2008-01
2 CHEN Jing,ZHANG Su-hua,DU Juan,et al.(1.Department of Hypertension and Endocrinology,Third Military Medical University,Chongqing 400042,China;2.Department of Endocrinology,First Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400016,China);Changes of blood glucose,serum lipids,leptin and the levels of hypothalamic leptin in diet-induced obese rats[J];Chongqing Medicine;2006-09
3 MU Hua,TIAN Zhi-qiang,ZHONG Jian,et al.(Department of Hypertension and Endocrinology,Daping Hospital,Third Military Medical Universtity,Chongqing 400042,China);Relationship between abdominal fat and ambulatory blood pressure monitoring[J];Chongqing Medicine;2006-09
4 WEI Bin,ZHAO Zhi-gang,CHEN Jing,et al.(Department of Hypertension and Endocrinology,Chongqing Institute of Hypertension,Daping Hospital,Center for Hypertension and Metabolic Diseases,Third Military Medical University,Chongqing 400042,China);Ultrasonography-determined visceral fat and its clinical significance[J];Chongqing Medicine;2006-09
5 YIN Qun-fang 1 reviewing,CHEN Dan 2 checking (1.Department of Nutrition;2.Department of Endocriology,Dongguan People's Hospital,Dongguan 523018,Guangdong,China);Mechanism and prevention of metabolic syndrome[J];Parenteral & Enteral Nutrition;2009-03
6 LUO Yi-zhou1,MIAO Dong-mei2,FU Ji-hua3(1.Oncology Department of the 454th Hospital of PLA,Nanjing 210002,Jiangsu,China;2.Intenal Department of Port Hospital,Nanjing 210011,Jiangsu,China;3.Department of Physiololgy,China Pharmaceutical University,Nanjing 210009,Jiangsu,China);Establishment of rat stress ulcer model induced by cold sea water-immersion and exhausted swimming[J];Journal of Southeast China National Defence Medical Science;2007-06
7 ZHU Zhi-ming (Department of Hypertension and Endocrinology,Daping Hospital,Third Military Medical University,Chongqing 400042,China);Metabolic hypertension:the real existence and clinical significance[J];World Clinical Drugs;2009-10
8 GENG Xue-chuan,ZHAO Xiao-hong,HAN Li-rong.Department of Neurology,Boxing peoples hospital,Shandong 256500,China;Relationship Between the Helicobacter Pylori Infection and the Blood-lipoids Metabolism in Cerebral Infarction Patients[J];Neural Injury and Functional Reconstruction;2010-01
9 WANG Xiao-mei,JIN Tao(Zaozhuang Municipal Hospital,Shandong Zaozhuang 277102,China);The Characteristics of Carotid Atherosclerosis in the Individuals at High Risk of Metabolic Syndrome[J];Hebei Medicine;2008-02
10 LUO Yi-zhou, FU Ji-hua, LIAO Hui, et al. Department of Oncology and Hematology, The 454th Hospital, PLA, Nanjing Jiangsu 210002, China;Effects of Immersion and Exhausting Swimming in Low Temperature Sea Water on Hematalogy and Functions of Liver and Kidney in Rats[J];Military Medical Journal of South China;2007-04
China Proceedings of conference Full-text Database 2 Hits
1 LIU Xiaoying HUANG Xiaozhong YU Xiyong Research Center for Medical Sciences,Guangdong Provincial People's Hospital,Guangzhou 510080;Development of animal model with metabolic syndrome[A];[C];2007
2 YU Hai-Bin1 ZHAO Min2 LI Jian-Sheng1 ( Beijing University of Traditional Chinese Medcine ,Beijing,100029 );The Kidney Pathological Change of Rat Model of Metabolic Syndrome and Effcet of Ageing[A];[C];2006
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 WANG Xiuqing,GAO Zhen,GUO Hong,et al.Lab of Military Pathology,Bethune military Medical College,Shijiazhuang 050081,China;Creation of Chronic Stress Hypertension Animal Model[J];Journal of Bethune Military Medical College;2003-04
2 WT5”BX]GUO Xiaohua,LU Zhihong,LI Hen,ZHU jiaming,LI Leishi Research Institute of Nephrology,Jinling Hospital,Nanjing University Clinical School of Medicine,Nanjing 210002;A NOVEL RAT MODEL OF TYPE 2 DIABETES MELLITUS[J];CHINESE JOURNAL OF NEPHROLOGY, DIALYSIS & TRANSPLANTATION;2000-04
3 LI Yi 1, WU Zhu tang 1, LIU Zhen 1, LI Ye 1, SU Wei 2, LIU Su mei 2 (1.Beijing Hospital of Ministry of Health,Beijing 100730,China;2.Institute of Laboratory Animals, Chinese Academy of Medical Sciences);Insulin Resistance and Hypertension Rat Model Induced by Fructose-rich and Its Features[J];Chinese Journal of Laboratory Animal Science;2004-04
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