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《Journal of Guangdong Pharmaceutical University》 2012-01
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Study on extraction process of total flavonoids from Gynostemma pentaphyllum

PAN Zhi-hao1,YE Zhen-zhen2,CUI Sheng-miao2(1.Chencun Hospital of Shunde,Foshan 528313,China;2.School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China)  
Objective To optimize the extraction process of total flavonoids from Gynostemma pentaphyllum.Methods The L9(34) orthogonal test was designed to investigate the effect of ethanol concentration,material-liquid ratio,extraction temperature and extraction time with the content of total flavonoids as index.UV-spectrophotometry was used for the determination of total flavonoids.Results The optimum conditions were as follows: 60% ethanol,material liquid ratio of 1∶60,extraction temperature at 90 ℃ and extracting time for 2 h.Conclusion The extraction process is feasible and suitable for extracting total flavonoids from Gynostemma pentaphyllum.
【Fund】: 广东药学院重点培养教师资助计划
【CateGory Index】: R284.2
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