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《Acta Polymerica Sinica》 2004-01
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LU Diannan, ZHOU Xuanrong, XING Xiaodong, WANG Xiaogong, LIU Zheng (Department of Chemical Engineering,Tsinghua University,Beijing\ 100084)  
Surface contact disinfecting technique is a newly developed method for water sterilization.An experimental protocol to elucidate the anti bacterial p r ocess and mechanism was proposed and applied to a novel surface contact disinfec tant made of cellulose fiber grafted with DMAE BC,using E.Coli,as a model b ac terium.The minimum inhibition concentration (MIC) of the monomer,defined as a concentration at which the growth of E.Coli.in water is completely inhibite d ,was determined by the plate counting method.MIC was then used as an index to ch aracterize disinfection capability of the monomers.The disinfection mechanism o f the monomer was further investigated by measuring the activity of β gala ctosid ase and observing the cell morphology with a scanning electron microscope (SEM). To elucidate the disinfection process and mechanism of the grafted fiber,the s teady state and dynamic adsorption of E.Coli.on the fiber was examined,resp e ctively.The TTC dehydrogenase activity and respiration activity of E.Coli.a dsor bed on the fiber surface were also determined.The results showed that the steri lization process by the grafted fiber was composed of two steps,in which the ads orp tion of E.Coli.bacteria on the fiber surface driven by electrostatic interac tion was a fast process and cell lysis was a relatively slow process.
【CateGory Index】: TU991.2
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