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《Acta Polymerica Sinica》 2015-01
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Synergistic Antitumor Effect of Cisplatin and siRNA Mediated by Polyethylenimine

Zi-xue Jiao;Jie Cen;Hua-yu Tian;Xue-si Chen;Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences;University of Chinese Academy of Sciences;  
Polyethylenimine( PEI) was utilized as a carrier for mediating anti-apoptotic siRNA( si Survivin),which could silence the expression of survivin gene. The si Survivin was used with an antitumor drug( cisplatin) for synergistic antitumor treatment. The siRNA binding capacity of PEI was evaluated by gel retardation assay,and the complete formation of PEI / siRNA was achieved at the weight ratio of 0. 4. The drug resistant ability was found to be eightfold higher in resistant cells compared with the sensitive cells,and the survivin gene was overexpressed in A549 DDPcells. RT-PCR experiment proved that PEI / si Survivin complex was capable of achieving an effective silencing effect of survivin gene,and the silencing effect did not change after the cisplatin was added. Cell apoptosis assay showed that the synergistic effect of cisplatin and gene could achieve a significant apoptosis rate of 60. 9%,while cisplatin or PEI / si Survivin complex was used alone,only about 30. 2% and 19. 8% of apoptosis rate could be obtained,respectively. Cell proliferation assays could further validate the effective synergistic antitumor effect of cisplatin and si Survivin mediated by PEI.
【Fund】: 国家自然科学基金(基金号51303173 51222307 51390480);; 吉林省科技发展计划项目(基金号20130521011JH)资助
【CateGory Index】: O631.3;TQ460.1
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