Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《高分子科学(英文版)》 2013-05
Add to Favorite Get Latest Update

A BIOREDUCIBLE POLYPEPTIDE FOR EFFICIENT GENE TRANSFECTION BOTH IN VITRO AND IN VIVO

Wei Qu a,Si Chen a,Shan Ren b,Xue-jun Jiang b,Ren-xi Zhuo a and Xian-zheng Zhang a** a Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry,Wuhan University,Wuhan 430072,China b Department of Cardiology,Renmin Hospital of Wuhan University,Wuhan 430060,China  
A disulfide bond containing polypeptide,PolyK 6-R 8,was designed and prepared by oxidative polymerization between terminal cysteinyl thiol groups of CHK 6 HC and CR 8 C.The molar ratio between CHK 6 HC and CR 8 C within obtained PolyK 6-R 8 was 9:1,and PolyK 6-R 8 could combine DNA compactly when weight ratio reached 5.Through in vitro investigation,it was found that PolyK 6-R 8 was an efficient gene vector with low cytotoxicity for delivering DNA in both COS-7 and HeLa cells.Cellular uptake of DNA mediated by PolyK 6-R 8 /DNA complexes was promoted after incubation for 4 h.Moreover,by examining the histological sections of hindlimb ischemia rats through immunohistochemistry,PolyK 6 R 8 /VEGF complexes were proved to be effective in both VEGF protein expression and succeeding vessel formation.The results indicated that polypeptide-based PolyK 6-R 8 is a promising gene vector for the limb ischemia treatment.
【Fund】: financially supported by the National Natural Science Foundation of China(Nos.51125014 51233003);; the Ministry of Science and Technology of China(2011CB606202)
【CateGory Index】: R450
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 Zhao Hong-pu1,Xu Qiu-yu2,Yang Fan3 1Department of Orthopaedic Surgery,Third Affiliated Hospital of Guangzhou Medical Collage,Guangzhou 510150,Guangdong Province,China;2Department of Outpatient,First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;3Department of Pharmacy,Guangdong College of Pharmacy,Guangzhou 510224,Guangdong Province,China;Intra-articular injection of polylactic acid microspheres containing compound Chinese herbal medicine into rabbits with knee osteoarthritis[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2010-51
2 ZHOU Zhi-Yi,REN Jian-Li,WU Qi,et al.Department of Cardiovascular,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China;Preparation and detection of two growth factors controlled-releasealginate hydrogel carrier[J];Chinese Journal of Gerontology;2011-08
3 JIA Yingbin,LI Jian,PAN Haiyan,SU Yonghui,GUAN Xiaodong,ZHANG Baimeng (Department of General Surgery,the Fifth Affiliated Hospital,Sun-yat University,Zhuhai,Guangdong 519000,China);Establishment of chronic limb ischemia model in rats and its comparison with acute chronic limb ischemia model[J];Chinese Journal of General Surgery;2011-12
4 Lin Xiao-hua, Huang Zong-hai, Yu Jin-long Department of General Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China;Alginate fiber: Development and biomedical application[J];Chinese Journal of Tissue Engineering Research;2013-12
5 WEI Xiaojuan1,2,XI Tingfei1,GU Qisheng3,ZHENG Yufeng2.1Academy for Advanced Interdisciplinary Studies,2College of Engineering,Peking University,Beijing,100871,P.R.China;3Shanghai Qisheng Institution of Biomaterials & Technology;PROGRESS OF ALGINATE-BASED BIOMEDICAL MATERIALS[J];Chinese Journal of Reparative and Reconstructive Surgery;2013-08
6 KANG Kai1,QU Hui2,TANG Ji-quan3,XIE Bao-dong1,JIA Zhi-bo1,ZHANG Yu-nan1,WU Hua1,LI Ren-ke1,JIANG Shu-lin1(. 1. Department of Cardiovascular Surgery,Second Affiliated Hospital of Harbin Medical University, Key Laboratory of Myocardial Ischemia Mechanism and Treatment established by Ministry of Education & Heilongjiang Province,Harbin 150086,P.R.China;2. Department of Pediatrics,Second Affiliated Hospital of Harbin Medical University, Harbin 150086,P. R. China;3. Department of Cardiothoracic Surgery,Jixi People's Hospital,Jixi 158100,P. R. China);Growth Factor-conjugated Collagen Patch Prolongs Survival Rate of Transplanted Cells after Ventricular Aneurysm Repair in Rats[J];Chinese Journal of Clinical Thoracic and Cardiovascular Surgery;2013-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved