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《高分子科学(英文版)》 2013-05
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Wei Qu a,Si Chen a,Shan Ren b,Xue-jun Jiang b,Ren-xi Zhuo a and Xian-zheng Zhang a** a Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry,Wuhan University,Wuhan 430072,China b Department of Cardiology,Renmin Hospital of Wuhan University,Wuhan 430060,China  
A disulfide bond containing polypeptide,PolyK 6-R 8,was designed and prepared by oxidative polymerization between terminal cysteinyl thiol groups of CHK 6 HC and CR 8 C.The molar ratio between CHK 6 HC and CR 8 C within obtained PolyK 6-R 8 was 9:1,and PolyK 6-R 8 could combine DNA compactly when weight ratio reached 5.Through in vitro investigation,it was found that PolyK 6-R 8 was an efficient gene vector with low cytotoxicity for delivering DNA in both COS-7 and HeLa cells.Cellular uptake of DNA mediated by PolyK 6-R 8 /DNA complexes was promoted after incubation for 4 h.Moreover,by examining the histological sections of hindlimb ischemia rats through immunohistochemistry,PolyK 6 R 8 /VEGF complexes were proved to be effective in both VEGF protein expression and succeeding vessel formation.The results indicated that polypeptide-based PolyK 6-R 8 is a promising gene vector for the limb ischemia treatment.
【Fund】: financially supported by the National Natural Science Foundation of China(Nos.51125014 51233003);; the Ministry of Science and Technology of China(2011CB606202)
【CateGory Index】: R450
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