EXPRESSION OF INTEGRIN-LIN KED KINASE IN NORMAL AND DIABETIC KIDNEYS AND ITS SIGNIFICANCE IN THE PATHOGENESIS OF GLOMERULOSCLEROSIS
Wang Zhaoxia, Chen Xiangmei, Shi Suozhu, Su Junxia, Yin Zhong, Zhang Xueguang, Wu Jie(Division of Nephrology, General Hospital of PLA, Kidney Center and Key Laboratory of PLA, Beijing 100853, China)
Objective To explore the expression of integrin-linked kinase (ILK) in normal and diabetic kidneys and its significance in the pathogenesis of diabetic glomerulosclerosis. Methods Immunohistochemistry and image analysis were wsed to detect ILK and fibronectin (FN) expression in renal biopsies from 14 cases with diabetic nephropathy and 3 cases of normal kidney. Results ILK was detected in podocytes and faintly expressed in tubular epithelial cell and mesangial cells of the normal kidney. In diabetic nephropathy, ILK was expressed in mesangial cells, podocytes and atrophied tubular epithelial cells. ILK level was reduced in nodular sclerosis and global sclerosis. There was a positive correlation between the expressions of ILK and FN at the early and medium stage of diabetic nephropathy (P0.001), but a negative correlation at its end stage (P0.05).Conclusion ILK expression in the mesangium is dramatically increased and correlated with that of FN, suggesting that ILK may increase extracellular matrix deposition and be involved in mesangial matrix expansion in the pathogenesis of diabetic glomerulosclerosis.