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《Chinese Journal of Histochemistry and Cytochemistry》 2005-02
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CONSTRUCTION OF EUKARYOTIC VECTOR CARRYING TGF-β1 GENE AND ITS EXPRESSION IN BONE MESENCHYMAL STEM CELLS

Wang Rong, Cao Yingguang~*,Wang Huajun,Wu Huihua (Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. Wuhan 430030, China);  
Objective To construct the eukaryotic vector containing TGF-β1, which was transfected intobone mesenchymal stem cells and expressed. Methods Plasmid pCDNA3.1(+) containing human transforming growth factor β1 gene was constructed and then transfected into the primary cultured rBMSCs under the mediation of Lipofectamine. Positive clones were selected by G418; the expression was verifiedby using RT-PCR, ELISA, and immunocytochemical staining; and the proliferation of the transfected BMSCs was evaluated by MTT. Results RT-PCR, ELISA and immounhistochemical staining of post-transgenic BMSCs were TGF-β1positive. MTT colorimetric methodshowed that the transfected TGF-β1 had a positiveeffect on the proliferationof BMSCs. Conclusion Transfection ofpCDNA3.1(+)-TGF-β1can provide persistent expression in BMSCs.
【Fund】: 武汉市科技攻关计划项目 (20036004)
【CateGory Index】: R346
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