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《CHINESE JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY》 1995-01
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STUDY ON THE QUANTITATIVE METHOD OF IMMUNOHISTOCHEMISTRY(Ⅲ)

Shen Hong(The First Military Medical University,Guangzhou,510515)  
An advanced quantitative method of immunohistochernical staining was developed,which brought about a new formula for testing and calculating positive unit(PU).According to this method,only the gray level and the area of defined area A and the phase a of positive products(or background 9) in A need to be tested; besides,the gray setting method needn't to be regarded.The formula is as follows:PU=100×|Ga-GA |/(AAβ.Gmax)=100×|Ga-GA|/[(1 -AAα).Gmax].G.and GA denote separately the mean gray level of the studying structure a and the test area A which equals area a plus area β in the formula;AAαand AAα mean respectively the area density of a and 5 in the A; Gmax is the maximum gray level of the instrument.Although the gray setting is free,the test of gray level should be performed in the same setting.The method could also be used for the reactive intensity of other histochemistry.cytochemistry and hybridization in situ.
【Fund】: 国家自然科学基金
【CateGory Index】: R392
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【Citations】
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1 Shen Hong Lu Yaodan (Department of Pathology, The First Military Medical University, Guangzhou 510515);Study on the Quantitative Method of Immunohistochemistry[J];生物医学工程学杂志;1993-04
【Co-citations】
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1 JIN Liyan;JIN Jianhua;LU Wenbin;YAO Juan;WANG Yue;Department of Oncology,Wujin Hospital,Jiangsu University;Institute of Oncology,Taizhou People 's Hospital,Jiangsu University;;The expression and significance of miR-33b and HMGA2 in esophageal squamous cell carcinoma[J];细胞与分子免疫学杂志;2016-09
2 CHEN Ren-hui;HE Song-guo;CAI Can-xin;HUANG Bo-xue;WANG Zhi-rong;Department of Criminal Sciences and Technology, Fujian Police College;Department of Pathology, Fujian Medical University;;Expression of Aquaporin 4 in Diffuse Brain Injury of Rats[J];法医学杂志;2016-01
3 Huang Yong;Lun Zengjun;Bao Guangjian;Chu Peng;Department of General Surgery,Zaozhuang Municipal Hospital;;Expression of AEG-1 and NF-kappa B in gastric carcinoma[J];中华普外科手术学杂志(电子版);2016-01
4 HUANG Yong;LUN Zeng-jun;BAO Guang-jian;ZHONG Xian-feng;CHU Peng;Department of General Surgery,Zaozhuang Municipal Hospital;;Expression of ERCCl and Survivin in colorectal carcinoma and its clinical significance[J];中国实用医刊;2015-14
5 CHEN Ren-hui;HE Song-guo;Department of Criminal Sciences and Technology of Fujian Police College;The Laboratory Center of Fujian Police College;;A Study on the Expression of Homer1 Protein After Diffusing Axonal Injury in Rats[J];福建警察学院学报;2014-05
6 CHEN Renhui;FAN Zhaohui;GAO Hui;Department of Criminal Sciences and Technology,Fujian Police Institute;Criminal Investigation Detachment of Public Security Bureau Cangshan Branch;Department of Pharmacology,Medical College of Jishou University;;Special Staining Pathologic Changes and the Expression of NF-κB After Diffuse Axonal Injury in Rats[J];吉首大学学报(自然科学版);2014-05
7 GUAN Kai-nian;XU Si-ri gu leng;ZHANG Yu-fei;WANG Zhuan-jia;LIU Shu-ying;College of Veterinary Medicine,Inner Mongolia Agricultural University;Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,The Ministry of Agriculture;;IMMUNOHISTOCHEMICAL DETECTION OF THE EXPRESSION OF ALDOLASE A IN OVINE PULMONARY ADENOMATOSIS[J];内蒙古农业大学学报(自然科学版);2014-04
8 HUANG Aili;LIU Shuguang;SHENG Wenjie;SHEN Hong;Department of Pathology,Nanfang Hospital,Southern Medical University;;Quantitative analysis of the expression and correlation of TGF-β1 and PTEN in non-small cell lung cancer tissues[J];中国体视学与图像分析;2014-01
9 LUO Qiong;SHAO Shaowei;LIU Shuguang;LEI Bin;SHEN Hong;Department of Pathology,College of Basic Medical Sciences,Southern Medical University;Department of Oncology,Leiyang People's Hospital;Department of Pathology,the Second Zhaoqing People's Hospital;;Quantitative analysis of the expression and clinical significance of P53 and Ki67 in cervical lesions[J];中国体视学与图像分析;2013-03
10 WANG Zhong-hua;DAI Gang;ZHOU Rong-liang;KUANG Ze-min;Department of Cardiology,Chenzhou First People's Hospital of Hunan Province;;The effect of recombinant human interleukin-2 activated natural killer cells on angiogenesis and cardiac function in rats with experimental myocardial infarction[J];中华心血管病杂志;2013-09
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Guan Meimei;Liu Changhao;Huang Miaoling;Department of Obstetrics and Gynecology,Sun Yat-sen Memorial Hospital,Sun Yat-sen University;;The study of correlation between the expression of estrogen receptor,progesterone receptor and the effect of hysteroscopic adhesiolysis with estrogen and progesterone therapy[J];现代妇产科进展;2014-06
2 Zhao Kailiang;Batur Niyaz;Yang Bo;Deng Wenhong;Zuo Teng;Wang Weixing;Department of General Surgery,Renmin Hospital of Wuhan University;;Effects of peroxisome proliferator-activated receptors-γ ligand on hyperlipidemic rats with severe acute pancreatitis[J];中华急诊医学杂志;2014-01
3 CHEN Deyu;JIN Liyan;ZHU Longping;MOU Xiao;WANG Shengjun;MAO Chaoming;Institute of Oncology,Affiliated Hospital of Jiangsu University;School of Medical Science and Laboratory Medicine,Jiangsu University;;Expressions and correlations of let-7a and IL-6 in esophageal squamous cell carcinoma[J];细胞与分子免疫学杂志;2013-11
4 ZHAO Jing,ZHANG Qiong,LI Yan-ping(Reproductive Medicine Center,Xiangya Hospital,Central South University,Changsha 410008,Hunan,China);The Role of Combined Endometrial Thickness and Pattern Measured by Ultrasonography in the Prediction of Clinical Outcome During IVF-ET Cycles[J];生命科学研究;2012-05
5 Ying-zhen Wang;Shi-wen Wang;You-cheng Zhang;Zhi-jiang Sun;Intensive Care Unit,Second Hospital of Gansu Province;Department of General Surgery,Lanzhou University Second Hospital;Intensive Care Unit,General Hospital of Lanzhou Petrochemical;;Protective effect of exogenous IGF-I on the intestinal mucosal barrier in rats with severe acute pancreatitis[J];World Journal of Emergency Medicine;2012-03
6 LIU Yu-huan1,ZHAO Yu-ting,JIANG Dong-qiao,XIA En-lan, LIU Lin-lin,SONG Dong-mei,ZHENG Jie,PENG Xue-bing (1 Fu Xing Hospital,Capital Medical University,Beijing 100038,P.R.China);Effect of high dose estrogen in prognosis of moderate or serious intrauterine adhesion[J];山东医药;2012-12
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10 CHEN Xiao-yan, WANG Wei-xing,DING You-ming,YIN Tao,CUI Zhou-jun,YU Jia.Department of Hepato-biliary and Laparoscopic Surgery,Renmin Hospital of Wuhan University,Wuhan 430060,China;Protective effect of rosiglitazone on liver injury in rats with severe acute pancreatitis[J];中华急诊医学杂志;2010-08
【Secondary References】
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1 REN Xueming;ZHU Yubo;Pharmacy of Traditional Medicine in Liaocheng Traditional Chinese Medicine Hospital;;The Effect on Dachaihutang of Inflammation Response and Oxidative Stress in Patients with Acute Pancreatitis of Liver-qi Stagnation Type[J];贵州医科大学学报;2017-07
2 CHEN Cui-jing;Department of Obstetrics and Gynecology, Guangdong Tongjiang Hospital;;Relationship between serum homocysteine, folic acid levels in early pregnancy and spontaneous abortion[J];中国实用医药;2017-18
3 GAO Shuang;WANG Jian-Fang;The Sixth People's Hospital of Huizhou City;;Effect of continuous administration of intrauterine water sac combined with large-dose estrogen on readhension,menstrual recovery,and pregnancy rate after transcervical resection of adhesion[J];中国妇幼保健;2017-12
4 YANG Yanlin;Department of Gynecology,Wuxi Hospital of Traditional Chinese Medicine;;Curative Observation of Using Guishen Pill Combined with Taohong Siwu Decoction in Improving the Prognosis of Intrauterine Adhesions[J];四川中医;2016-12
5 CAO Guangxin;LI Junfeng;ZHANG Guangping;ZHANG Chuanguang;LIU Yongdong;HAN Jianqiang;SHANG Xifeng;HU Qiang;Department of Vascular Surgery,Weifang People's Hospital;Department of Pediatric Surgery,Weifang People's Hospital;Weifang Medical University;;Expression of Connective Tissue Growth Factor in Kidney Tissue of Children Patients with Hydronephrosis Caused by UPJO[J];贵州医科大学学报;2016-10
6 LI Guangmei;DENG Shujing;SU Yu;Department of Clinical Laboratory,Maternal and Child Health Hospital of Wuchuan City of Guangdong Province;Department of Obstetrics and Gynecology,Maternal and Child Health Hospital of Wuchuan City of Guangdong Province;;The relationship between peripheral blood coagulation factor and unexplained recurrent spontaneous abortion[J];检验医学与临床;2016-12
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