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Shen Hong(The First Military Medical University,Guangzhou,510515)  
An advanced quantitative method of immunohistochernical staining was developed,which brought about a new formula for testing and calculating positive unit(PU).According to this method,only the gray level and the area of defined area A and the phase a of positive products(or background 9) in A need to be tested; besides,the gray setting method needn't to be regarded.The formula is as follows:PU=100×|Ga-GA |/(AAβ.Gmax)=100×|Ga-GA|/[(1 -AAα).Gmax].G.and GA denote separately the mean gray level of the studying structure a and the test area A which equals area a plus area β in the formula;AAαand AAα mean respectively the area density of a and 5 in the A; Gmax is the maximum gray level of the instrument.Although the gray setting is free,the test of gray level should be performed in the same setting.The method could also be used for the reactive intensity of other histochemistry.cytochemistry and hybridization in situ.
【Fund】: 国家自然科学基金
【CateGory Index】: R392
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