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《Agricultural Research in the Arid Areas》 2015-03
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Establishment and optimization of two-dimensional gel electrophoresis for proteome of Glycyrrhiza uralensis

CAO Jun-mai;ZHANG Xin;College of Life Science & Engineering,Beifang University of Nationalities;  
Callus of Glycyrrhiza uralensis was used in this research for extraction of total proteins,SDS-PAGE,loading quantity and staining method,allowing optimization of the two-dimensional gel electrophoresis(2D-E) technology. The results showed that improved acetone precipitation could increase the protein contents and image resolution of gel electrophoresis. There were 1 132 protein spots identified using silver staining when the loading quantity of the sample protein was 450 μg/(17 cm IPG trip). This research developed a 2D-E method applicable for protein analysis of Glycyrrhiza uralensis callus.
【Fund】: 宁夏自然科学基金资助项目“甘草脱分化过程中细胞结构变化的研究”(NZ0953)
【CateGory Index】: S567.71
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