Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Agricultural Research in the Arid Areas》 2017-03
Add to Favorite Get Latest Update

Study on key enzyme activity in nitrogen metabolism and the content of molybdenum and iron in alfalfa under different NO_3~--N/NH_4~+-N ratio

HAO Feng;LIU Xiao-jing;FAN Jun-jun;College of Grassland Science,Gansu Agricultural University/Key Laboratory of Pratacultural Ecosystem,Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-US Center for Grazingland Ecosystem Sustainability;  
By solution culture method,‘Gannong No. 3'alfalfa was chosen as the experimental material to explore the effects of NO_3~--N/NH_4~+-N ratios( 1/7、1/3、3/5、5/5、5/3、3/1、7/1) based on nitrogen supply of210 mg·L-1on the activity of nitrate reductase( NR) and nitrogenase,and the content of molybdenum( Mo) and iron( Fe) in organs of alfafa during the whole growth period. The results showed that:( 1) NR activity and Mo content was consistent: leaf root stem,while Fe content was root leaf stem. With the proportion of NO_3~--N increasing,NR activity in each organ became higher. In the early stage,when NO_3~--N/NH_4~+-N equaled to 7/1,NR activity was the highest. The influence of different ratio on Mo content in root during seedling stage was similarto NR activity,that is to say,in large proportion of NO_3~--N it was the highest. During budding stage,the same as nitrogenase activity,in 1/7 it was highest. During mature stage,same as nitrogenase and NR activity,in 5/3 it was highest. The influence of different ratio on Fe content in root was similar to nitrogenase activity,that is to say,it was highest at 1/7 in seedling and 5/3 from full-bloom to mature.( 2) NR and nitrogenase activity showed a single-peak curve during the whole growth period,that is to say,NR activity was the highest during full-bloom stage and nitrogenase activity was the highest during podding stage. NR activity was higher under larger proportion of NO_3~--N in different ratios during the whole growth period. Nitrogenase activity was the highest in 1/7 at seedling and budding,and from full-bloom to mature it was the highest in 5/3. Mo and Fe content in the stem and leaf was consistent,they were the highest for larger proportion of NH_4~+-N from budding to full-bloom and highest for larger proportion of NO_3~--N from podding to maturity.( 3) Relationship between NR and nitrogenase activity differed in the whole growth period. At the early growth stage( from seeding to budding),NR activity was highest in 7/1 and lowest in 1/7. Nitrogenase activity was in the opposite situation,suggesting that there was an antagonistical relationship between NR and nitrogenase activity. From full-bloom to mature,both NR and nitrogenase activity was highest in 5/3,they accelerated each other.
【Fund】: 国家自然科学基金项目(31460622);; 公益性行业(农业)科研专项(201403048-8);; 甘肃省科技支撑项目(1504NKCA003)
【CateGory Index】: S541.9
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 NAN Li-li;YU Ji-hua;GUO Quan-en;College of Pratacultural Science,Gansu Agricultural University/Ministry of Education Key Laboratory of Ecosystem/Gansu Province Laboratory of Pratacultural Engineering / Sino-US Center for Grazingland Ecosystem Sustainability;College of Agronomy,Gansu Agricultural University;Soil Fertilizer and Water-saving Agriculture Institute,GAAS;;The change of soil enzyme activities in alfalfa field with different growing years in arid desert oasis[J];干旱地区农业研究;2015-06
2 Hao Feng;Liu Xiaojing;Zhang Xiaolei;Qi Minxing;College of Grassland Science/Key Laboratory of Grassland Ecosystem of Ministry of Education/Sino-U.S.Centers for Grazing land Ecosystem Sustainability,Gansu Agricultural University;;Effect of Complex Saline-alkali Stress on Nitrogen and Phosphorus Absorption and Physiological Traits of Alfalfa Seedlings[J];中国沙漠;2015-05
3 LIU Xiao-Jing;YE Fang;ZHANG Xiao-Ling;College of Grassland Science,Gansu Agricultural University;Key Laboratory of Grassland Ecosystem of Ministry of Education / Sino-U. S. Centers for Grazing Land Ecosystem Sustainability;;Effects of exogenous nitrogen forms on root characteristics of alfalfa at different growth stages[J];草业学报;2015-06
4 YE Fang;LIU Xiao-jing;ZHANG Jin-xia;College of Grassland Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem,Ministry of Education/Sino-U.S.Centers for Grazing Land Ecosystem Sustainability;;Effects of Nitrogen Forms on the Nitrogen Metabolism of Alfalfa'Gannong No.3' Varity at Different Growth Stages[J];草地学报;2015-02
5 Liu Xiaojing;Zhang Jinxia;Li Wenqing;Fan Junjun;College of Pratacultural Science/Ministry of Education Key Laboratory of Grassland Ecosystem/Sino-U.S.Center for Grazing Land Ecosystem Sustainability,Gansu Agricultural University;Gansu Provincial Sci.& Tech.Department;;Effects of Nitrogen and Phosphorus Addition and Cuttings on Yield and Quality of Alfalfa in Dry Region of Gansu,China[J];中国沙漠;2014-06
6 Yu Ling;Ma Huiling;College of Pratacultural/ Ministry of Education Key Laboratory of Grassland Ecosystem/Sino-U.S.Center for Grazingland Ecosystem Sustainability,Gansu Agricultural University;;The Endogenous Hormone Level and Drought Adaptability of Four Kentucky Bluegrass Species in Gansu,China[J];中国沙漠;2015-01
7 LI Kai;GUO Yu-qi;LIU Chu-nan;LU Xing;LIAO Hong;Root Biology Center,College of Resource and Environment Science,South China Agricultural University;;Effects of different NH_4~+/NO_3~- ratios on soybean growth,nodulation and biological N fixation[J];中国油料作物学报;2014-03
8 JIN Feng-xia;MA Dong-mei;LIU Hao-yan;YANG Peng-peng;XU Xing;Agricultural College, Ningxia University;;Effects of planting years of alfalfa on soil quality[J];干旱地区农业研究;2014-02
9 LIU Zhao-fan1,ZHANG Guo-bin1,YU Ji-hua1,YANG Hai-xing2,SHI Gui-ying1,MA Yan-xia1,LI Jie1(1College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;2Yuzhong Agricultural Technology Promotion Center,Yuzhong 730100,Gansu,China);Effects of different nitrogen forms and their ratios on broccoli yield,quality,and nutrient absorption[J];应用生态学报;2013-07
10 HU Ying,HUANG Yi-zong*,HUANG Yan-chao,LIU Yun-xia(Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China);Formation of Iron Plaque on Root Surface and Its Effect on Cd Uptake and Translocation by Rice(Oryza sativa L.)at Different Growth Stages[J];农业环境科学学报;2013-03
【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 SUN Yingying;SUN Xubo;CAO Tingting;ZHANG Haiou;Shaanxi Land Construction Group Co.,Ltd.;Institute of Land Engineering & Technology,Shaanxi Provincial Land Engineering Construction Group,Co.,Ltd.;Key Laboratory of Degraded and Unused Land Consolidation Engineering the ministry of Land and Resources of China;;Study on the Influence of Soft Rock on Moisture Storage Capacity of Sandy Soil[J];水土保持研究;2017-06
2 TAO Shuang;HUA Xiaoyu;WANG Yingnan;GUO Na;YAN Xiufeng;LIN Jixiang;Key Laboratory of Saline-alkali Vegetation Ecology Restoration,Ministry of education,Research Center for Biological Resources and Environment in Saline-alkali Soil,Northeast Forestry University;Department of Food and Environmental Engineering, East University of Heilongjiang;;Research Advance in Effects of Different Nitrogen Forms on Growth and Physiology of Plants[J];贵州农业科学;2017-12
3 WANG Li-xue;WU Hong-xin;HUO Wen-juan;FENG Jing;MENG Fan-rui;Tianjin Animal Science and Veterinary Research Institute;Tianjin Technology and Engineering Center of Livestock and Poultry Healthy Breeding;Grassland Research Institute of CAAS;Tianjin Academy of Agricultural Sciences;;Study on the Relationships between Nutrition Quality of Medicago sativa and Climate Factors[J];中国草地学报;2017-06
4 TANG Jianmin;SHI Yancai;ZOU Rong;XIONG Zhongchen;JIANG Yunsheng;WEI Jiqing;Guangxi Key Laborary of Functional Phytochemicals Research and Utilization,Guangxi Institute of Botany,Chinese Academy of Sciences;;Effects of Different Fertilization Treatments on Yield and Quality of Flos Sophorae Immaturus[J];广西科学院学报;2017-04
5 Chen Runjuan;Lei Yawei;Bai Xiaoming;Tian Yanfeng;College of Grassland Science/Key Laboratory of Grassland Ecosystem of Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S.Centers for Grazingland Ecosystem Sustainability,Gansu Agricultural University;;Effect of NO on Cold Resistance of Several Wild Kentucky Bluegrass Germplasm Materials[J];中国沙漠;2017-06
6 Wei Jing;Agricultural Technology Promotion Center of Longkou City of Shandong Province;;Simple Analysis of the Effect of Saline-alkali Stress on Seed Germination and Seedling of Oats[J];黑龙江八一农垦大学学报;2017-05
7 SHAN Tian-yu;LIU Qiu-xin;YAN Xiu-lan;SHAO Jin-qiu;LIAO Xiao-yong;Beijing Key Laboratory of Environmental Damage Assessment and Remediation, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences;Key Laboratory of Land Surface Pattern and Simulation;University of Chinese Academy of Sciences;;Cd and As absorption and transport characteristics of rice in a paddy field co-contaminated by Cd and As[J];农业环境科学学报;2017-10
8 YU Tie-feng;LIU Xiao-jing;ZHANG Xiao-ling;HAO Feng;College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Pratacultural Ecosystem,Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-US Center for Grazingland Ecosystem Sustainability;;Effects of nitrogen on nitrogen content and nitrate reductase activity in root stem and leaf of alfalfa[J];草原与草坪;2017-05
9 XU Zixian;LI Yinshui;HAN Peipei;LIAO Xiangsheng;HU Xiaojia;XIE Lihua;YU Changbing;ZHANG Xiurong;LIAO Xing;QIN Lu;Oil Crops Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetics Improvement of Oil Crops of the Ministry of Agriculture;;Effect of Different NH_4~+-N/NO_3~--N Ratios on the Photosynthetic and Fluorescence Characteristics of Sesame Seedlings[J];河南农业科学;2017-09
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 BAI Li-guo;YU Ling;MA Hui-ling;College of Pratacultural Science,Gansu Agricultural University,Key Laboratory of Grassland Ecology System,Ministry of Education,Sino-U.S.Centers for Grazingland Ecosystem Sustainability;;Physiological response of wild Poa pratensis to drought stress[J];草原与草坪;2014-02
2 NAN Li-li;SHI Shang-li;ZHANG Jian-hua;College of Pratacultural Science,Gansu Agricultural University,Key Laboratory of Grassland Ecosystem of Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing land Ecosystem Sustainability;;Study on root system development ability of different root-type alfalfa[J];草业学报;2014-02
3 XU Guo-wei;LI Shuai;ZHAO Yong-fang;CHEN Ming-can;LI You-jun;Agricultural College,Henan University of Science and Technology;;Effects of straw returning and nitrogen fertilizer application on root secretion and nitrogen utilization of rice[J];草业学报;2014-02
4 PENG Lan-qing;LI Xin-yong;QI Xiao;YUE Yan-hong;FAN Shu-gao;LI Shu-cheng;WANG Yan-rong;State Key Laboratory of Grassland Agro-ecosystems,College of Pastoral Agriculture Science and Technology,Lanzhou University;National Animal Husbandry Service,Office of the Chinese Herbage Cultivar Registration Board;;The relationship of root traits with persistence and biomass in 10 alfalfa varieties[J];草业学报;2014-02
5 Cui Xing;Shi Shangli;College of Grassland Science/Key Ecosystem Laboratory of the Ministry of Education/Sino-US Center for Grazing land Ecosystem Sustainability/Pratacultural Engineering Laboratory of Gansu Province,Gansu Agricultural University;;Effects of Cropping Patterns on Soil Aggregates of Alfalfa Field[J];中国沙漠;2014-02
6 WANG Bao-ping;DONG Xiao-yan;DONG Kuan-hu;College of Animal Science and Veterinary Medicine,Shanxi Agricultural University;;Effects of Saline-alkali Stress on the Physiological Characteristics of Alfalfa Seedlings[J];草地学报;2013-06
7 WANG Dan;HE Feng;XIE Kai-yun;WAN Li-qiang;LI Xiang-lin;Institute of Animal Sciences,Chinese Academy of Agricultural Sciences;;Effects of nitrogen application on alfalfa plant growth and soil nitrogen content[J];草业科学;2013-10
8 LIU Xiao-jing1,LIU Yan-nan1,KUAI Jia-lin2,QI Min-xing1,ZHANG Xiao-lei1(1.Key Laboratory of Grassland Ecosystem,Ministry of Education/College of Pratacultural Science,Gansu Agricultural University /Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S.Centers for Grazingland Ecosystem Sustainability,Lanzhou, Gansu Province 730070,China;2.Gansu Academy of Agricultural Sciences,Lanzhou,Gansu Province 730070,China);Effects of Different N Levels on Productivity and Quality of Alfalfa Varieties[J];草地学报;2013-04
9 YIKIM Maynur1,ZHANG Bing-chang2,SULAYMAN Mamtimin1(1.College of Life Science and Technology,Xinjiang University,Urumqi 830046,China;2.Key Laboratory of Biogeography and Bioresources in Arid Land,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China);Seasonal Variations of Microbial Biomass and Soil Enzyme Activity in Biological Soil Crusts in the Gurbantunggut Desert[J];中国沙漠;2013-04
10 LIU Yan-nan1,LIU Xiao-jing1,ZHANG Xiao-lei1,QI Min-xing1,KUAI Jia-lin2(College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S.Centers for Grazingland Ecosystem Sustainability,Lanzhou 730070,China;2.Gansu Academy of Agricultural Sciences,Lanzhou 730070,China);Effect of clippingand fertilization on production performance of different alfalfa varieties[J];草原与草坪;2013-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved