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《HIGH TECHNOLOGY LETTERS》 2000-07
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Construction of Vector for Oilseed Rape Chloroplast Transformation and Its Insecticidal Toxicity

Hou Bingkai, Zhang Zhonglin , Zhou Yihua Zhang Yinmei, Shen Guifang , Chen Zhenghua (Institute of Genetics, Chinese Academy of Sciences, Beijing 100101) ( Biotechnology Research institute, Chinese Academy of Agricultural Sciences, Beijing  
WT5BZ]1.0 kb and 2.4 kb DNA fragment containing rps7 gene and ndhB gene, respectively, were cloned from oilseed rape chloroplast genome. A full length 3.5 kb Bt toxin gene ( cry1Aa ) was also cloned from the plasmid of Bacillus thuringiensis. The vector containing Bt toxin gene and aadA gene for oilseed rape chloroplast transformation was constructed with the rps7 gene and ndhB gene as homologous recombinant fragments. Bioassays using crude expressed products from host strain of the vector showed that Bt toxin gene was expressed and its products were toxic to larvae of Heliothis armigera. The chloroplast transformation vector will be of great value to oilseed rape chloroplast transformation and to the insect resistant crop improvement.
【Fund】: 国家自然科学基金!(39970624)
【CateGory Index】: Q943
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【References】
Chinese Journal Full-text Database 10 Hits
1 WU Yu-yong et al(Genetics Staff Room,Binzhou Medical College,Yantai,Shandong 264003);Construction of Chloroplast Multicistron Single-cross Expression Vector in Brassica napus L.[J];Journal of Anhui Agricultural Sciences;2008-11
2 Zhou Yihua, Shi Dongqiao, Hou Bingkai, Xiao Yuhong, Zhang Zhonglin , Zhanglihua, Sheng Guifang , Hu Zanmin, Chen Zhenghua (Institute of Genetics, Chinese Academy of Sciences, Beijing 100101) ( Biotechnology Research Institute, Chinese Academy;Cloning of Two DNA Fragments from the Chloroplast Genome of Populus tomentosa and Construction of Its Site-directed Chloroplast Transformation Vectors[J];High Technology Letters;2001-09
3 PAN Weidong,ZHANG Guixing,YUAN Baomei, LI Jie, WANG Jianmin, XUE Lexun Laboratory for Cell Biology, Zhengzhou University, Zhengzhou 450052;Cloning of 16S rRNA sequence of the Dunaliella salina chloroplast[J];Journal of Zhengzhou University(Science Medical);2004-01
4 PAN Weidong,L Yumin,ZHANG Guixing,NIU Xiangli,HOU Guiqin,XUE Lexun Laboratory for Cell Biology , Zhengzhou University, Zhengzhou 450052;Transcription activities of the atpA promoter of Chlamydomonas reinhartii chloroplast in Dunaliella salina[J];Journal of Zhengzhou University(Science Medical);2004-01
5 GENG Xiao-xia,WANG Wen-chao,HOU Bing-kai*(The Key Lab of Plant Cell Engineering and Germplasm Innovation,Ministry of Education and School of life Science,Shandong University,Jinan 250100,Shandong,China);The construction and prokaryotic expression of a site-specific integration vector for chloroplast transformation of spinach(Spinacia oleracea L.)[J];Journal of Shandong University(Natural Science);2010-01
6 HOU Bing kai 1,2 ,XIA Guang min 1,CHEN Zheng hua 2 (1.College of Life Sciences,Shandong University,Jinan 250100,China; 2.Institute of Genetics, Chinese Academy of Sciences,Beijing 100101,China);Strategies for Optimizing Expression Vectors Used in Plant Genetic Engineering[J];Hereditas(Beijing);2001-05
7 FENG Ying,XUE Qing-zhong (Department of Agronomy,Agriculture and Biotechnology College,Zhejiang University,Hangzhou,310029,China);Genetic Engineering of Crop for Insect Resistance and Its Biosafety[J];Hereditas(Beijing);2001-06
8 HOU Bing-kai 1,YU Hui-min 2,XIA Guang-min 1 (1.School of Life Science,Shandong University,Jinan 250100,China; 2.Department of Biology,Shandong Education College,Jinan 250013,China);Expression Vector Used in Chloroplast Genetic Transformation[J];Hereditas(Beijing);2002-01
9 LI Hong-Tao,ZHAO Shu-Qing,ZHAO Yan-Xiu, ZHANG Hui (Key Lab of Salt Stress Plants ,Shandong Normal University , Jinan 250014 ,China);The Introduction of Chloroplast Gene Engineering[J];Hereditas(Beijing);2003-04
10 HOU Bing-Kai * HU Zan-Min ** DANG Ben-Yuan SHI Rui CHEN Zheng-Hua(Institute of Genetics, Chinese Academy of Sciences, Beijing 100101);Site-specific Integration of Insect-resistant Gene into Chloroplast Genome of Oilseed Rape and Acquisition of Transgenic Plants[J];Acta Photophysiologica Sinica;2002-03
【Citations】
Chinese Journal Full-text Database 8 Hits
1 Hou Bingkai\ Chen Zhenghua (Institute of Genetics,Chinese Academy of Sciences,Beijing\ 100101);The Plastid Transformation in Higher Plants[J];PROGRESS IN BIOTECHNOLOGY;2000-01
2 Zou Zhurong Zhang Zhonglin Shan Song Ni Pichong Shen Guifang (Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing, 100081);Construction of Vector for Tobacco Plastid Transformation and Obtainment of Tobacco Transgenic Plants[J];Acta Agronomica Sinica;1998-04
3 Shan Song Zhang Zhonglin Chen Xi Liu Chunying Shen Guifang(Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing, 100081);Cloning and Modification of 16S Promoter of Tobacco Chloroplast Genome and Production of Transgenic Plants[J];Acta Agronomica Sinica;1999-05
4 Zhang Zhonglin Shan Song Chen Xi Liu Chunying Qian Kaixian Shen Guifang(Biotechnology Research Center, Chinese Academy Academy of Agricultural Sciences, Beijing 100081; Department of Bio-science and Technology, Zhejiang University, Hangzhou 310027);Herbicide Resistance Gene (bar) was Introduced into the Chloroplast of Tobacco[J];Acta Agronomica Sinica;1999-05
5 LI Xue-Bao; ZHENG Shi-Xue; DONG Wu-Bei; CHEN Guang-Rong(College of he Sciences, Central China Normal University, Wuhan 430079)MAO Hui-Zhu; BAI Yong-Yan(Shanghai Institute of Plant Physiology Chinese Academy of Sciences shanghai 200032);Insect-resistant Transgenic Plants of Brassica napus and Analysis of Resistance in the Plants[J];ACTA GENETICA SINICA;1999-03
6 ZHANG Zhong-Lin; REN Yan-Guo; SHEN Yan-Xin; SHAN Song; FAN Guo-Chang'; WU Xiang-Fu'; QMN An-Xian; SHEN Gui-Fang Biotechnology Research Center, Chinese Academy Of Agricultural sciences, Beijing 100081, China Department of Bioscience and Technology, Cheji;Expression of Bacillus thuringiensis (Bt) Crystal Toxin Gene in the Chloroplast of Tobacco[J];ACTA GENETICA SINICA;2000-03
7 Wang Wugang Wu Kongming Liang Gemei (Institute of Plant Protection, CAAS, Beijing 100094) Li Xiuli (Qiliying Experimental Station of Xinxiang County, Henan);Occurrence of cotton pests in the Bt cotton fields and its control strategy[J];PLANT PROTECTION;1999-01
8 ZHANG Zhong_Lin 1CHEN Xi 2QIAN Kai_Xian 1SHEN Gui_Fang 1(Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing 100081) 2(Department of Bioscience and Technology, Zhejiang University, Hangzhou;Studies on Insect Resistance of Bt Transplastomic Plants and the Phenotype of Their Progenies[J];ACTA BOTANICA SINICA;1999-09
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 FAN Guang-hua,LI Dong-gang*(Dezhou Academy of Agricultural Sciences,Dezhou 253015,China);Effect of Different Ecological Factors on Occurence of Lygocoris lucorum Meyer-Dur on Insect-resistant Cotton[J];Shandong Agricultural Sciences;2008-02
2 GUI Teng-qin et al(Department of Chemistry & Biology,Southwest Guizhou Teachers College for Nationalities,Xingyi,Guizhou 562400);Progress of Studies on Chloroplast Genetic Engineering[J];Journal of Anhui Agricultural Sciences;2009-08
3 QIN Qiu ju, LI Guo ping, YANG Xiang dong, HE Yun zhuan (College of Plant Protection,Agricultural University of Hebei,Baoding 071001,China);Effects of transgenic Bt cotton on population dynamic of cotton bollworm and their natural enemies[J];Journal of Agricultural University of Hebei;2002-02
4 LI Wen xiang 1,LIU shun 1,WEI Guo shu 1,ZHAO Bin tao 2(1. College of Plant Protection, Agricultural University of Hebei, Baoding 071001,China;2. Department of Agriculture and Forestry, Vocational Technology College of Baoding, Hebei 071051,China);Influences of transgenic Bt cotton upon arthropod community and analysis of potential risk about it[J];Journal of Agricultural University of Hebei;2003-S1
5 GUO Li~(1),DUAN Jin-xiu~(1),LIANG Zhan-xun~(2),SUN Xiao-ling~(1)ZHANG He-shan,WANG Xiao-xing~(3),CAO Zhi-zhong~(1)(1.Pratacultural College of Gansu Agricultural University,Lanzhou 730070,China;2.Gansu Green Ecological Company,Lanzhou 730000,China;3.Sichuan Chuanda Guangyao Bioengineering Ltd,Chengdu 610031,China);Plant expressing vector construction of MAPK gene in Dunaliella salina and transformation to tobacco[J];Grassland and Turf;2006-04
6 WANG Li-jun~1, FAN San-hong~2,GUO Ai-guang~(2*) (1 College of Life Science, Northwest Sci-Tech University of Agriculture and Foresf Shaanxi Province, Yangling, Shaanxi 712100,China);Cloning and function analysis of ats1A gene promoter from Arabidopsis thaliana[J];Acta Botanica Boreali-occidentalia Sinica;2004-10
7 KAI Guo yin, XU Ming, ZHENG Hui yong (College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Advances in genetic transformation of sweet potato[J];Journal of Fujian Agricultural University(Natural Science);2001-02
8 DAI Xiao-yan1,2,SU Yi-rong2,FAN Ye-kuan1,HUANG Dao-you2(1 College of Resource and Enviroment,Huazhong Agriculture University,Wuhan 430070,China;2 Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China);Application of genetic engineering in tobacco study[J];Journal of Guangxi Agricultural and Biological Science;2008-01
9 DING Jiangang, LI Chengqiong, SONG Hongyuan (Horticulture & Park College, Southwest Agri. Uni., Chongqing 400716, China);High-efficiency regeneration system for cabbage (Brasssica oleracea L. Var capitata) hypocotyls[J];Guangxi Agricultural Science;2003-05
10 DUAN Fa-ping, LIANG Cheng-ye, LI Yuan-qing [WT8.5BZ]( South China Institute of Botany, the Chinese Academy of Sciences, Guangzhou 510650, China );Research advances of Bar gene and its transgenic crops[J];Guihaia;2001-02
China Proceedings of conference Full-text Database 5 Hits
1 Yang guangdong, Li yan'e. Shangguan Xiaoxia (Cotton research institute, Shanxi academy of agricultural science, Yuncheng 044000);Study on the Optimization of Transformation System in Chinese Cabbage[A];[C];2000
2 CAO Gui-yan,LIU Yan-zhen,SUN Chang-jun(Institute of Industrial Crops, Liaoning Academy of Agriculture,Liaoyang 111000,China);Insect-resistance of Transgenic Bt Cotton and Strategy of Preventing Insect[A];[C];2005
3 MA Yan, PAN Deng-ming, PENG Jun (Cotton Research Institute, CAAS, Anyang, Henan 455112, China);Efficacy of New Kinds of Pesticides on Controlling Aphids (Aphis gossypii Glover) in Transgenic Bt Cotton Field[A];[C];2004
4 MA Xiao-Mu, LIU Xiao-Xia, ZHANG Qing-Wen LI Ji-Jun, JIANG Zhen-Qi (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China; Handan Academy of Agriculture Sciences, Handan, Hebei 050061, China; Beijing Agricultural Bureau, Beijing 100029, China);A study on relationship of weed strips at cotton field edges and population dynamics of cotton spider mites (Tetranychus cinnabarinus Boisduval and T.truncatus Ehara)[A];[C];2005
5 MA Hui,ZHOU Yu,ZHAO Ming,WANG Hong-yan,XIA Xiao-ming~* (Cotton Research Center,Shandong Academy of Agricultural Sciences,Jinan 250100,China);Occurrence and Integrated Control of Cotton Spider Mites in Transgenic Bt Cotton Field[A];[C];2009
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhang Xi1,2 Chen Cunshe2 Li Chengwei3 Wang Lei1 (1Biotechnology Research Institute,Chinese Academy of Agricultural Science,Beijing 100081;2 College of Chemistry and Environment Engineering,Beijing Technology and Business University,Beijing 100037;3 Shangqiu Normal University,Henan 476000);Cloning of a cDNA Sequence Encoding △~(12)-fatty Acid Desaturase Gene(FAD2) from Brassica Napus and Its Transformation to Tobacco[J];Anhui Agricultural Science Bulletin;2007-11
2 XU Ben-bo et al (Chongqing Rapeseed Engineering Research Center,College of Agronomy and Biotechnology,Southwest University,Chongqing 400716);Influence of Co-culture Media on Transient Expression of GUS Gene and Transformed Efficiency of Hypocotyls of Rapeseed(Brassica napus L.) Mediated by Agrobacterium tumefaciens[J];Journal of Anhui Agricultural Sciences;2007-25
3 LIU Changjing 1,WU Ding 2 (1.Wuxi Light Industry University ,Jianshu Wuxi 214036;2.Anhui Agrotechnical Teachers College,Anhui Fengyang 233100);Production of Pharmaceutical Protein with Plant Genetic Engineering[J];JOURNAL OF ANHUI AGROTECHNICAL;2000-01
4 Lin Shanzhi; Xiao Jihu; Zhang Zhiyi.Institute of Populus tomentosa , Beijing For. Univ., 100083,P.R.China.;Development in resistance gene engineering researches of poplars.[J];Journal of Beijing Forestry University;2000-06
5 Zheng Junbao; Zhang Yuman;Yang Wenzhi(Hebei Forestry College, Baoding 071000)Pei Dong(The Reserch Institute of Forestry CAF,Beijing 100091)Tian Yingchuan;Mang Keqiang (Institute of Microbiology GAS, Beijing 10080);Plant Regeneration of Excised Leaf from 741 Poplar and Transformation with Insect-Resistant B.t.toxin Gene[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1995-03
6 Fu Xiangxiang, Jiang Wenbo, Shi Jisen(Nanjing Forestry University, Nanjing 210037, P. R. China);Molecular Study on Genetic Variation of Sycamore Provenances[J];Journal of Northeast Forestry University;2005-01
7 Liu Beidong 1 Zhu Yanming 2 Li Haiyan 2 Yang Qian 1 (1 Department of Life Science and Engineering ,Harbin Institute of Technology, Harbin 150001; 2 Life Science Collage ,Northeast Agriculture University, Harbin 150030);STUDY ON FACTORS AFFECTING THE SOYBEAN COTYLEDONARY NODE REGENERATION[J];Soybean Science;2002-02
8 YANG Shu ying,ZHANG Jian xin,LU Jia long,SHI Rong li\j (Institute of Life Science,Northwest Sci Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Studies on effect of exogenous betaine on physiological index of drought resistance for winter wheat[J];Acta Botanica Boreali-occidentalia Sinica;2000-06
9 WANG Yao,LIU Sheng,DU Tao,LIN Mu lan * (Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China);Transformation of Agrobacteria tumefaciens on healthy and infectious Paulownia[J];Acta Botanica Boreali-occidentalia Sinica;2001-03
10 SUN Wen yue,WANG Hui,HUANG Jiu chang * (College of Life Science,Lanzhou University,Lanzhou 730000,China);The effect of external betaine on membrane lipid peroxidation of wheat seedling under water stress[J];Acta Botanica Boreali-occidentalia Sinica;2001-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WANG Jing-yi et al (College of Forestry and Horticulture,Henan Agricultural University,Zhengzhou,Henan 450002);Construction of Plant Expression Vector for Isopentenyltransferase (ipt) Gene Expressed mainly in Fruit of Actinidia deliciosa[J];Journal of Anhui Agricultural Sciences;2007-16
2 WU Yu-yong et al(Genetics Staff Room,Binzhou Medical College,Yantai,Shandong 264003);Construction of Chloroplast Multicistron Single-cross Expression Vector in Brassica napus L.[J];Journal of Anhui Agricultural Sciences;2008-11
3 LI Sha-sha et al(College of Forestry and Horticulture,Henan Agricultural University,Zhengzhou,Henan 450002);Research Progress of the Application of Specific Promoter in ipt Gene Transformation[J];Journal of Anhui Agricultural Sciences;2008-14
4 QI Yan,LI Jie,ZHU Yan-ming,BAI Xi(College of Life Science,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Construction of bivalent plant expression vector with rip gene and tcs gene[J];Journal of Northeast Agricultural University;2006-01
5 GUO Yushuang1,ZHANG Yanju2,ZHU Yanming1,LIU Jia2, LI Jie1,BAI Xi1,ZHANG Shuzhen2(1.College of Life Sciences,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC; 2.College of Agriculture,Northeast Agricultural University,Harbin Heilongjiang 150086,PRC);Resistance identification of fungi-resistant genes transformed soybean to Phytophthora sojae[J];Journal of Northeast Agricultural University;2006-06
6 Zhang Yan, Yang Chuanping, Wang Yucheng(School of Forestry, Northeast Forestry University, Harbin 150040, P. R. China);Construction of Two Gene Plant Expression Vector with Metallothionein Genes from Tamarix androssowii and Puccinellia tenuiflora and Its Expression in Tobacco[J];Journal of Northeast Forestry University;2007-05
7 GUO Yushuang~1 ZHANG Yanju~2 ZHU Yanming LIU Jia~2 LI Jie~1 BAI Xi 1 College of Life Sciences,Northeast Agricultural University,Harbin 150030,China 2 College of Agriculture,Northeast Agricultural University,Harbin 150030,China;Resistance identification of bivalent fungi-resistant genes transformed soybean to Phytophthora sojae[J];东北农业大学学报(英文版);2007-03
8 WANG Dan,WANG Pi-wu,FU Yong-ping,LI Zhi(College of Agriculture,Jilin Agricultural University,Changchun 130118,Jilin,China);Cloning and Identification of the Root Specific Promoter from Soybean[J];Soybean Science;2009-02
9 WU Guo-hua,ZHANG Qiang(Key Laboratory of Animal Virology of Ministry of Agriculture,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu,730046,China);Advance in Transgenic Plant Edible Vaccine against Foot-and-mouth disease[J];Progress in Veterinary Medicine;2008-04
10 Li Xinqi 1,2 Yuan Longping 1,2* Xiao Jinhua 2 Deng Qiyun 21 Biological Science and Technology College, Central South University, Changsha, 4100832 China National Hybrid Rice Research & Development Center, Changsha, 410125;Novel Approaches to Utilize Crop Heterosis Based on Plastid Transformation[J];Molecular Plant Breeding;2004-06
China Proceedings of conference Full-text Database 1 Hits
1 Wang Changtao~1 Zhao qi~2 Yang Aiguo~1 Zhao Yujin~1 Zhang Shihuang~(1**) 1 CA.AS,The Institute of Crop Sciences, Maize Research Center, Beijing,100081; 2 Capital Normal University, Beijing, 100037;Genetic transformation of superoxide dismutases gene (Fe-SOD) by Agrobacterium tumefaciens-mediated in maize[A];[C];2004
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 Shen Jinliang\ Zhou Weijun\ Wu Yidong\ Lin Xiangwen(Department of Plant Protection, Nanjing Agricultural University Nanjing 210095)Zhu Xiefei(Department of Agronomy, Nanjing Agricultural University Nanjing 210095);EAPLY RESISTANCE OF HELICOVERPA ARMIGERA (HBNER) TO BACILLUS THURINGIENSIS ANDITS RELATION TO THE EFFECT OF TRANSGENIC COTTON LINES EXPRESSING BT TOXIN ON THE INSECT[J];ACTA ENTOMOLOGICA SINICA;1998-01
2 Ren Yanguo Zou Zhurong Zhang Zhonglin Yu Xiaolan Shan song Shen Guifang (Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing, 100081);Establishment of Tobacco Plastid Genetic Transformation Via Bombardment Microprojectiles[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1997-01
3 Li Xuebao;Chen Guangrong;Jin Bo(Department of Biology, Normal University of Central china Wuhan 430070);PLANT REGENERATION AND TRANSFORMATION OF LINUM USITATISSIMUM HYPOCOTYL IN VITRO[J];JOURNAL OF WUHAN BOTANICAL RESEARCH;1995-04
4 Zou Zhurong Zhang Zhonglin Shan Song Ni Pichong Shen Guifang (Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing, 100081);Construction of Vector for Tobacco Plastid Transformation and Obtainment of Tobacco Transgenic Plants[J];Acta Agronomica Sinica;1998-04
5 Guo Sandui(Lab. Molecular Biology, Biotechnology Research Center, Chinese academy of Agricultural Sciences, Beijing 100081);ENGINEERING OF INSECT-RESISTANT PLANT WITH BACILLUS THURINGIENSIS CRYSTAL PROTEIN GENES[J];SCIENTIA AGRICUTURA SINICA;1995-05
6 Wang Wugang Jiang Yongxing Yang Xuemei Guo Yuyuan (Institute of Plant Protection,Chinese Academy of Agicultural Sciences,Beijing 100094) Guo Sandui Ni Wanchao (The center of bio technology, CAAS) (Institute;Study on Evaluation and Utilization of Bt Transgenetic Cotton Resistant to Cotton Bollworm[J];SCIENTIA AGRICUTURA SINICA;1997-01
7 CHENG Zhen-Dong;WEI Zhi-Ming;XU Zhi-Hong Shanghai Institute of Plant Physiology, Academia, Sinica, Shanghai 200032;Genetic Transformation of Brassica Crops[J];Plant Physiology Communications;1992-03
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