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《High Technology Letters》 2001-03
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Cloning and Characterization of a Rice Small GTP-binding Protein Gene Osrab5B

Lin Huixian, Liu Xiaobin, Li Faqiang, Luo Wenyong , Liu Liangshi (The Key Laboratory of Gene Engineering of Ministry of Education, State Key Laboratory for Biocontrol, Zhongshan University, Guangzhou 510275) (  The Rice Research Institute, Guangd  
Small GTP binding proteins (SGPs) are involved in regulating intracellular vesicular transport by activation of vesicle formation and fusion of inner membrane. By means of screening a rice panicle cDNA library with one map position known genomic BAC clone as probe, an EST encoding a fragment of a SGP was obtained. Based on the sequence of this EST, the electronic cloning was performed with another 3 ESTs. According to the result, the primers were designed and a cDNA clone was got, named Osrab5B , by means of RT-PCR. Osrab5B contains 1016 bp and shows high similarity to the rab5B genes from Mesembryanthemum crytallinum and Lotus japonicus, all of which belong to a new subfamily of rab family. Further more, new primers were designed and the genomic sequence of Osrab5B was obtained. Our analysis indicates that it includes at least 7 introns and 8 exons.
【Fund】: 86 3计划!(BH 0 1 0 2 0 3);; 国家教育部重点科学技术项目!(0 6 1C6 15 );; 广东省水稻生物技术育种与应用资助项目
【CateGory Index】: Q943.2
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