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《High Technology Letters》 2001-09
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Using Transgenic Chlorella ellipsoidea as Bio-reactor to Produce Rabbit Defensin

Wang Yiqin, Chen Ying, Bai Qinhua, Zhao Shimin, Li Wenbin, Sun Yongru (Institute of Genetics, Chinese Academy of Sciences, Beijing 100101)  
The gene of rabbit defensin (NP 1) a cationic antimocrobial peptide was transformed into unicellar eukaryotic alga Chlorella ellipsoidea. After antibiotic screening, molecular and activity detection, several transgenic alga with stable expression was obtained. Also the expressed rabbit defensin with bio activity was isolated from transgenic Chlorella ellipsoidea. This paper was the first report that express defensin successfully in transgenic Chlorella ellipsoidea which established a new system for defensin production in low cost by the way of genetic engineering.
【Fund】: 国家“九五”科技攻关资助项目 (96 C0 2 0 4 0 5 )
【CateGory Index】: TQ465
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