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《High Technology Letters》 2003-12
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Effects of Nitrogen Sources and Initial NaHCO_3 Concentrations on the Growth and Thymosin α1 Expression of Transgenic Synechococcus sp. PCC7942

Zhu Du, Li Yuanguang, Ye Qin, Wei Xiaodong, Zhang Jun ( State Key Laboratory of Bioreactor Engineering, Institute of Marine Bioprocess Engineering, East China University of Science and Technology, Shanghai 200237) ( School of Life Science, Xiamen University, Xiamen 361005)  
Effects of nitrogen sources and initial NaHCO 3 concentrations on the growth and thymosin α1 expression of transgenic Synechococcus sp. PCC7942 were studied. The results showed that NaNO 3 was the optimum nitrogen. When NaNO 3 concentration was lower than 1g/L, increasing NaNO 3 concentration could stimulate alga cells growth and improve thymosin α1 expression. While the growth and the thymosin α1 expression were not affected significantly when NaNO 3 concentrations were increased from 1g/L to 2.5g/L. The optimum concentration of NaHCO 3 was 8.0g/L. The cell density and the maximum thymosin α1 expression capability of transgenic Synechococcus sp. PCC7942 growing in the medium containing 8.0g/L NaHCO 3 were 1.94 times and 2.01 times what were obtained in BG-11 medium respectively.
【Fund】: 863计划 ( 819 Q0 1);; 上海市科技启明星计划;; 上海重点学科资助项目
【CateGory Index】: Q943
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