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《HIGH TECHNOLOGY LETTERS》 1998-01
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The Pilot Studies of Recombinant Staphylokinase for Injection

Yu Min, Zhang Xiaoxuan, Ren Jun, Cai Xu, Sun Cuie Zhu Yunsong, Song Houyan (Department of Molecular Genetics, Shanghai Medical University,Shanghai 200032)  
An effective and rapid pilot production procedure of recombinant staphylokinnase was established on the basis of the construction of the high expression engineering bacterium. After a step of gel filtration and a step of ion exchange, the qualified semi finished product of recombinant staphylokinase for injection was obtained. The recoveries of both proteins and activity were over 40%. Moreover, as the radial flow column in ion exchange chromatography was employed, the procedure was easy to be scaled up to the industrial production of r SAK.
【Fund】: 863计划资助
【CateGory Index】: Q552,
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