Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《The Chinese Journal of Clinical Pharmacology》 2001-02
Add to Favorite Get Latest Update

The Detection of Derepressed AmpC Enzyme and Extended-Spectrum β-Lactamases in Enterobacter Cloacae

WU Wei-Yuan, CHEN Min-Jun, WANG Hui (Department of Clinical Laboratories, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Beijing 100730)  
OBJECTIVE:Derepressed hyperproduction of the AmpC β-lactamase and/or production of extended-spectrum β-lactamases (ESBLs) have been documented as a prevalent mechanism of β-lactam resistance in Enterobacter cloacae strains in the world. METHODS: To establish a modified threedimensional extract test, which was used to detect phenotypically isolates which habor derepressed AmpC and/or extended-spectrum β-lactamases (ESBLs). In the test, the surface of a Mueller - Hinton agar plate was inoculated with E. coli ATCC 25922 as described in NCCLS for disk diffusion method. RESULTS:A cefoxitin disk (30μg/disc) was placed on the center of inoculated agar. 3 slits were cut around the edge of the disk in the agar in outward radial directions, and each of them was filled with crude enzyme extracted from each strain. The diffusion of AmpC enzyme from the slit intersected with the diffusion of cefoxitin disk resulting in the inactivity of cefoxitin because of hydrolysis by AmpC enzyme. Enhanced growth of the surface organism at the edge of zone of inhibition was considered to be a positive three-dimensional test and was interpreted as evidence for derepressed hyperproduction of the AmpC β-lactamase. This phenomenon could be inhibited by the addition of cloxacillin in the slit. Because ESBLs could not be inhibited by cloxacillin in the same way, ESBLs was detected after cefoxitin disc was replaced with ceftriaxone disc combining with cloxacillin as AmpC enzyme inhibitor. We detected 24 isolates of Enterobacter cloacae and used 5 standard strains which harbored AmpC enzyme or ESBLs as controls. It showed that, all standard strains were detected correctly. Among 24 strains of E. cloacae, 14 isolates were considered as deprepressed hyperproducting AmpC enzyme, 4 strains were ESBL-producing, 5 strains produced both of them. The rest multi-resistant strain was neither AmpC nor ESBL. CONCLUSION: The modified three-dimensional extract test could be used to detect the derepressed hyperproduction of the AmpC β-lactamase, or the production of ESBLs, or both of them.
【CateGory Index】: R446.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 Fang Ping, Pan Xiaolong, Zhou Dongsheng, et al Department of Infectious Diseases, Tongling People’s Hospital, Anhui Tongling 244000;Study on β-lactamases type of Gram-negative bacilli in Tonglfing[J];Anhui Medical Journal;2005-05
2 NIU Bo1,LIU Qun1,LI Mei1,LI Feng2,CHEN Li-zhong1,WANG Min1(1.Department of Clinical Laboratories,The Second Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233040,2.Department of Clinical Laboratories,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,China);Observation of bacteria drug resistance in urinary tract infection[J];Journal of Bengbu Medical College;2010-08
3 LI Xing lu,HUANG Chang wu,MA Jun. (Department of Clinical Laboratory,the Second Affiliatel Hospital,Chongqing University of Medical Sciences.Chongqing 400010,China);AmpC of the E.cloacae,E.areogenes and serratiawas detected and their drug resistance was analyzed[J];Chongqing Medical Journal;2003-03
4 QIU Jun feng,HUANG Chang wu (Department of Clinical Laboratory,the Second Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400010,China);The analysis of resistance for the proteus to commonly used antibiotics[J];Chongqing Medical Journal;2003-12
5 Bao Chunmei, Cui Enbo, Guo Tongsheng, et al. 302 Hospital of PLA, Beijing 100039, China;Drug resistance and ESBLs producing in 1831 Shigella sonnei strains[J];Infectious Disease Information;2009-03
6 ZHU Hai-Jiao,RAO Guan-Li,ZHANG De-Zhong.People′s Hospital of Qingtian,Qingtian,Zhejiang 323900,China;Antimicrobial resistance of Escherichia coli isolated from different samples in children[J];Chinese Journal of Contemporary Pediatrics;2010-05
7 LIAO Wei-jiao,JIANG Jie-hua,YI Jian-yun,XU Yun-jian,PAN Si-zeng.Department of Clinical Laboratory,The First Affiliated Hospital of Guangzhou Medical College,Guangzhou 510120,China;The appearance of a novel CMY type class C cephalosporinase (AmpC) in southern China[J];Journal of Chinese Physician;2006-05
8 ZHANG Bao-cai (Department of Clinical Laboratory,General Hospital of Zhongyuan Oil Field,Henan Poyang457001China);Detection Bacteria of Producing Ampe Enzyme,ESBLs and Analysis of Their Drug Resistant[J];Journal of Dali College;2003-01
9 FENG Fu-ying LAN Xiao-peng YANG Xian-yue ZHANG Ya-bin HU Xin-lan GUO Rong-ying PLA Center of Laboratory Medicine of Fuzhou General Hospital.Fezhon 350025,China.Department of Clinical Laboratory.Fujian Provincial Hospital.Fuzhou 350002,China;THE ANALYSIS OF PLASMID-MEDIATED AMPC ENZYME GENOTYPE AND EPIDEMIOLOGY OF ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE[J];Journal of Fuzhou General Hospital;2007-03
10 MING De-song, WU Yi-bo, XIE Zun jin (Department of Laboratory Medicine, the First Hospital of Quanzhou, Quanzhou 362000, China);Multi-substrates synergy-antagonize test for detecting extented-spectrum β-lactamases and AmpC β-lactamases[J];Chinese Journal of Infection Control;2003-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 FANG Ping~1, YU Xing-zhi~2(1.Department of Infectious Diseases, Tongling People's Hospital Anhui Province ,Tongling 244000;2.Department of Infectious Diseases,The First Affiliated Hospital Of Anhui Medical University, Hefei 230022);Advances in the studies and clinical treatment on the extended spectrum beta-lactamases and AmpC beta-lactamases[J];Anhui Medical and Pharmaceutical Journal;2005-08
2 NIU Bo1,LIU Qun1,LI Mei1,LI Feng2,CHEN Li-zhong1,WANG Min1(1.Department of Clinical Laboratories,The Second Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233040,2.Department of Clinical Laboratories,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,China);Observation of bacteria drug resistance in urinary tract infection[J];Journal of Bengbu Medical College;2010-08
3 LU Chun-yu, ZHANG Zheng (Department of Clinical Laboratory, Peking University People's Hospital, Beijing 100044);Effect of efflux system on multi-resistance of Acinetobacter baumannii[J];Beijing Medical Journal;2007-06
4 LI Xing lu,HUANG Chang wu,MA Jun. (Department of Clinical Laboratory,the Second Affiliatel Hospital,Chongqing University of Medical Sciences.Chongqing 400010,China);AmpC of the E.cloacae,E.areogenes and serratiawas detected and their drug resistance was analyzed[J];Chongqing Medical Journal;2003-03
5 QIU Jun feng,HUANG Chang wu (Department of Clinical Laboratory,the Second Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400010,China);The analysis of resistance for the proteus to commonly used antibiotics[J];Chongqing Medical Journal;2003-12
6 CAI Yu-jiao 1,LIU Lan 2, LIU Chang-lin 2,et al. (1.Department of Molecular Genetics,the Third Military Medical University,Chongqing 400038,China; 2.Department of Clinical Laboratory,the Children′s Hospital, Chongqing University of Medical Sciences, Chongqing 400014, China);Status of drug resistance and detection of the AmpC enzyme,ampC gene expression in non-fermenting gram-negative bacilli[J];Chongqing Medical Journal;2005-05
7 LIU Ding-yuan,HU Bo,WANG Jie,et al.(Sui Ning People's Hospital of SiChuan,SiChuan 629000,China);Analysis to resist drug of streptococcus peumonia in children infection[J];Chongqing Medicine;2007-22
8 Qu fen, Cui Enbo, Guo Tongsheng, et al. 302 Hospital of PLA, Beijing 100039, China;Variability and antimicrobial resistant tendency of Shigella spp. groups and serotypes in diarrhea patients during 14 years[J];Infectious Disease Information;2007-04
9 Bao Chunmei, Cui Enbo, Guo Tongsheng, et al. 302 Hospital of PLA, Beijing 100039, China;Drug resistance and ESBLs producing in 1831 Shigella sonnei strains[J];Infectious Disease Information;2009-03
10 LIAO Wei-jiao,JIANG Jie-hua,YI Jian-yun,XU Yun-jian,PAN Si-zeng.Department of Clinical Laboratory,The First Affiliated Hospital of Guangzhou Medical College,Guangzhou 510120,China;The appearance of a novel CMY type class C cephalosporinase (AmpC) in southern China[J];Journal of Chinese Physician;2006-05
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Chuzhou First People's Hospital,Chuzhou 239000,Anhui CHEN Feng,LI Wei-chun,ZHANG Ke-chang;Analysis of Distribution and Antibiotic Resistance of 122 Positive Results of Blood Cultures in NICU[J];Journal of Anhui Health Vocational & Technical College;2012-02
2 Fang Ping, Pan Xiaolong, Zhou Dongsheng, et al Department of Infectious Diseases, Tongling People’s Hospital, Anhui Tongling 244000;Study on β-lactamases type of Gram-negative bacilli in Tonglfing[J];Anhui Medical Journal;2005-05
3 Zhang Handong, Li Xu 1. Department of Infectious Diseases, the People' s Hospital of Susong County, Susong 246500 2. Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, Hefei 230022;Distribution of Genotypes in ESBLs-producing Escherichia coli[J];Anhui Medical Journal;2009-02
4 Yang Haifei,Cheng Jun,Hu Lifen,et al Department of Infectious Diseases,the First Affiliated Hospital,Anhui Medical University,Hefei 230022,China;Antimicrobial resistance of AmpC-producing serratia marcescens in Anhui[J];Anhui Medical Journal;2012-02
5 LIN Lin,FANG Ping(Tongling People's Hospital,Tongling Anhui 244000);Bacteriologic analysis on infection of lower respiratory tract after intubations and tracheotomy[J];Anhui Medical and Pharmaceutical Journal;2006-08
6 YANG ZHi-ren(Department of Pulmonary Medicine,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,China);Efficacy of latamoxef in combination with roxithromycin in treating bronchiectasis with pulmonary infection[J];Anhui Medical and Pharmaceutical Journal;2010-12
7 XU Sheng-bao,DUAN Bao-xi(Department of Respiratory Disease,The Friendship Hospital of Anhui Province,Hefei,Anhui 233011,China);Antibiotic resistance of gram-negative bacteria from 84 patients with hospital acquired lower respiratory tract infection[J];Anhui Medical and Pharmaceutical Journal;2011-08
8 WU Hua1,PEI Ya-ling1,LIU Jian-hua1,HU Gong-zheng1,PANG Yu-shan1,CHEN Hua2,XU Kun-ling2,LI Ling-feng2(1.Engineering College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou 450002;2.Henan Muxiang Animal Drugs Co Ltd,Zhengzhou 450002);Determination of ESBLs and antibiotics susceptibility test analysis for avian pathogenic Escherichia coli from the disease duck[J];Journal of Anhui Agricultural University;2008-03
9 CHEN Ling1,ZHANG Tao2,GU Jin-hua1(1.Department of Laboratory,Kunshan First People's Hospital,Kunshan Jiangsu 215300; 2.Department of Microbiology and Parasitology,Bengbu Medical College,Bengbu Anhui 233030,China);Observation of Klebsiella pneumoniae infection in neonatal ward and analysis of the drug resistance[J];Journal of Bengbu Medical College;2011-02
10 ZHANG Cai-yun,RUI Fang,WANG Yan(The Third Hospital of Baoding City,Baoding 071000,China);Epidemiologic Model of Gram-negative Aerobic Bacteria in Hospitalized Cancer Patients[J];Labeled Immunoassays and Clinical Medicine;2011-02
China Proceedings of conference Full-text Database 3 Hits
1 (xu jing-feng xu-lin , fan wei-hong General Hospital of Beijing Military Command, Beijing 100700);Study on Antibiotics resistance and resistant genes of Acinetobacter Baumannii[A];[C];2009
2 xu jing-feng xu-lin,fan wei-hong General Hospital of Beijing Military Command,Beijing 100700;Study on Antibiotics resistance and resistant genes of Acinetobacter Baumannii[A];[C];2010
3 Chen Ke-fan Feng Xiu-juan L(u|¨) Xiao-ju Center of Infectious Diseases,West China Hospital,Sichuan University,Chengdu,Sichuan 610041;Clinical analysis of 70 cases with urinary tract infections caused by Escherichia coli producing extended spectrum of β-lactamase[A];[C];2010
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved