Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《The Chinese Journal of Clinical Pharmacology》 2011-03
Add to Favorite Get Latest Update

Determination the concentrations of phenobarbital,sodium valproate,phenytoin sodium and carbamazepine in human plasma for simultaneous quatification by HPLC-MS

DAI Bo,ZHANG Hua-feng,SONG Qing,XU Fang-chu(Department of Pharmacy,Air Force General Hospital of PLA,Beijing 100142,China)  
Objective To establish a liquid chromatography-mass spectrometry(HPLC-MS) method for determination the concentration of phenobarbital(PB),sodium valproate(VPA),phenytoin sodium(DPH) and carbamazepine(CBZ) in human plasma.Methods Mycophenolic acid was used as internal standard.After one step protein precipitation,the analytes were chromatographed on a XDB-C18(2.1 mm×150.0 mm,3.5μm).An isocratic mobile phase consisting of 0.05% ammonium acetate in water methanol(47∶53),flow velocity is 0.2 mL·min-1,column temperature is 30 ℃.ESI electrospray ionization source in both negative and positive modes.Results The calibration curves of each drug showed a good linearity in a range of concentration between 0.5-80.0 μg·mL-1for phenobarbital,2.5-200.0 μg·mL-1 for sodium valproate,0.5-40.0 μg·mL-1for phenytoin sodium,and 0.2-24.0 μg·mL-1 for carbamazepine,all γ0.99.Intra and inter precision(RSD%) were less than 3% and 15%,respectively.The recoveries were above 70%.Conclusion The method was suitable to determine these four drugs in human plasma.
【CateGory Index】: R971.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 4 Hits
1 YANG Hua, LIU Yi-ge (Xiamen Zhongshan Hospital of Fujian Province, Fujian Xiamen 361004, China);A review over adverse drug reaction of anti-epileptic drugsOU[J];Journal of Pediatric Pharmacy;2005-04
2 LUO Jiang-nan1,TAN Li2,CAO Ling2,WANG Yu1,2(1.China Pharmaceutical University,Nanjing 210009,China;2.Jiangsu Institute forFood and Drug Control,Nanjing 210008,China);Determination of 15 Antiepileptic and hypnotic compounds illegally adulterated into Traditional Chinese Medicines by LC-MS/MS[J];Chinese Journal of Biochemical Pharmaceutics;2010-03
3 Wang Xian-qin1,Lin Dan1,Pan Xiao-jun2,Lin Guan-yang3,Zhang Yuan1,Li Yan-xia1,Wang Xue-bao1,Ye Fa-qin1*(1.Analytical and Testing Center of Wenzhou Medical College,Wenzhou 325035,China;2.School of Pharmacy of Wenzhou Medical College,Wenzhou 325035,China;3.The First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China);Determination of phenytoin sodium in blood by LC-APCI-MS/MS[J];Chinese Journal of Health Laboratory Technology;2008-12
4 ZHU Yun - gui ,LI Hai - ju, WANG Feng, XIAO Yi -wen,ZHANG Bi -kui ,LI Huan -de ( Clinical Pharmaceutical Research Institute,Xiangya Second Hospital,Central South University,Changsha 410011 ,China; Postgraduate of Clinical Pharmaceutical Research Institute, Xiangya Second Hospital, Central South University ,Changsha 410011 , China );HPLC-MS/ESI Determination of Valproic Acid in Human Serum[J];Chinese Journal of Pharmaceutical Analysis;2005-07
【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 CHAI Jian-nong (No.1 People's Hospital of Changshu,Jiangsu Changshu 215500,China);A study on combined administration of lamotrigine and valproic acid sodium in treatment of children with intractable epilepsy[J];Journal of Pediatric Pharmacy;2008-03
2 ZHANG Lan,WANG Zeng-shou,ZHU Guang-hui,JIANG Shuo-min,ZHANG Hui,CUI Xiao,WU Wei-ming(The Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325027,China);Retrospect of the blood concentration of monitoring for antiepileptic drugs in our hospital[J];Strait Pharmaceutical Journal;2007-08
3 Wang Lin 1a,Qi Xiaolian 1b,Gao Ran 1a(1aNeuro-Intensive Care Unit,1bDepartment of Pharmacy,Xuanwu Hospital of Capital Medical University,Beijing 100053,China);Observation on safety of prolonged treatment with high blood phenobarbital concentration for acute encephalitis with refractory,repetitive partial seizures in 5 patients[J];Adverse Drug Reactions Journal;2009-01
4 HUANG Ling1, YU Sheng-Yuan2, LI Zhu-Yi1(1Department of Neurology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038; 2Department of Neurology, Chinese PLA General Hospital, Beijing 100853);STUDY ON THE ANALGESIC EFFECT OF ANALGECINE ON TRIGEMINAL NEURALGIA[J];Chinese Journal of Pain Medicine;2008-02
5 LI Xueqing1,SUO Lixia2,SHAO Yajun3 (1.Department of Pharmacy,Weihai Golden Bay Hospital,Group of Daqing Oil-Fields Total Hospital,Weihai 264200,China; 2.Department of Medical,Weihai Golden Bay Hospital,Group of Daqing Oil-Fields Total Hospital,Weihai 264200,China; 3.Department of Nursing,Weihai Golden Bay Hospital,Group of Daqing Oil-Fields Total Hospital,Weihai 264200,China);Carbamazepine reasonable application and poisoning first aid[J];China Medical Herald;2009-32
6 LI Xiao-jun (Cangxi People' s Hospital, Guangyuan 628400, China);Compared of the Efficacy about Epilepsy Patent Treated by Carbamazepine and Topiramate[J];Guide of China Medicine;2010-12
7 YANG Xue-zhi1,CHEN Guo-qian1,WANG Xian-qin2,ZHOU Qi3 (1.Department of Neurology,The First Affiliated Hospital,Wenzhou Medical College,Wenzhou 325000,China;2.Analytical and Testing Center,Wenzhou Medical College,Wenzhou 325035,China;3.Department of Stomatology,The Second People′s Hospital of Yongjia County,Wenzhou 325113,China);LC-ESI-MS determination of sodium valproate in human plasma[J];Chinese Journal of Health Laboratory Technology;2010-06
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 WANG Dan, CHEN Xiao - yan, ZHANG Yi - fan, LI Xiao - yan, KONG Zhang, ZHONG Da - fang ( Shanghai Institute of Materia Medi-ca, Chinese Academy of Sciences, Center for Drug Metabolism and Pharmacoki-netics Research, Shanghai 201203, China; Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University,Shenyang 110016, China );Determination the concentration of phenytoin in healthy human plasma and study on its bioequivalence by LC/MS/MS[J];The Chinese Journal of Clinical Pharmacology;2006-03
2 Wang Ping,Ren Rong-na.Department of Pediatrics,Fuzhou General Hospital,Nanjing Military Area Command of Chinese PLA,Fuzhou 350025,China;Adverse effects of antiepileptic drugs[J];International Journal of Internal Medicine;2007-02
3 XU Mei, ZHAO Hui, WAN Shao-hui, YANG Hao 1, Wang Hong-zhi 2(1. Pharmaceutical College of Henan University, Kaifeng Henan 475001,China;2. Henan Pharmaceutical School, Kaifeng Henan 475003,China);Determination of PB,DPH and CBZ concentration in the human plasma with HPLC[J];;2004-04
4 LI Ying(Anhui Traditional Chinese Medicine Hospital,Hefei 230031);Determination of Phenobarbital,Phenytoin and Carbamazepine in Serum by RP-HPLC[J];Qilu Pharmaceutical Affairs;2007-09
5 ZHU Quan, YUAN Xian-rui, SUN Zhi-dong (Dept of Neurosurgery, Xiangy a Hospital, Central South University, Changsha 410008, China);The epidemiological and sociological analysis, current situation and our strategies of the epilepsy[J];Journal of Shanxi Medical University(Preclinical Medical Education Edition);2005-02
6 Chen Jian(Chen J),Zhu Weiqun(Zhu WQ),Fang Weijun(Fang WJ),et al(Shanghai Songjiang Center Hospital,Shanghai 201600);Simultaneous determination of phenobarbital,phenytion and carbamazepine in serum by HPLC[J];CHINESE JOURNAL OF MODERN APPLIED PHARMACY;1999-04
7 GAO Qing,CHE Bao-quan,ZHANG Zhe,DAI Hong,GUO Hong-zhuBeijing Institute for Drug Control,Beijing 100035,China;Identification of 16 sedative-hypnotic drugs in traditional Chinese medicines and health foods by LC-MS/MS[J];Journal of China Pharmaceutical University;2008-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved