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《The Chinese Journal of Clinical Pharmacology》 2011-03
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Determination the concentrations of phenobarbital,sodium valproate,phenytoin sodium and carbamazepine in human plasma for simultaneous quatification by HPLC-MS

DAI Bo,ZHANG Hua-feng,SONG Qing,XU Fang-chu(Department of Pharmacy,Air Force General Hospital of PLA,Beijing 100142,China)  
Objective To establish a liquid chromatography-mass spectrometry(HPLC-MS) method for determination the concentration of phenobarbital(PB),sodium valproate(VPA),phenytoin sodium(DPH) and carbamazepine(CBZ) in human plasma.Methods Mycophenolic acid was used as internal standard.After one step protein precipitation,the analytes were chromatographed on a XDB-C18(2.1 mm×150.0 mm,3.5μm).An isocratic mobile phase consisting of 0.05% ammonium acetate in water methanol(47∶53),flow velocity is 0.2 mL·min-1,column temperature is 30 ℃.ESI electrospray ionization source in both negative and positive modes.Results The calibration curves of each drug showed a good linearity in a range of concentration between 0.5-80.0 μg·mL-1for phenobarbital,2.5-200.0 μg·mL-1 for sodium valproate,0.5-40.0 μg·mL-1for phenytoin sodium,and 0.2-24.0 μg·mL-1 for carbamazepine,all γ0.99.Intra and inter precision(RSD%) were less than 3% and 15%,respectively.The recoveries were above 70%.Conclusion The method was suitable to determine these four drugs in human plasma.
【CateGory Index】: R971.6
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