An in-vitro study of the targeting ability of the CD13 targeted fluorescein-NGR-gadolinium agent for tumor fluorescent and MR imaging
CHEN Xianfei;TU Rong;YOU Xiaoguang;Department of Radiology of hospital affliated to Hainan medical college;
Objective To evaluate targeting ability of a novel MR contrast agent, fluorescein-NGRgadolinium to CD13 receptor of tumors in vitro. Methods Fluorescein-NGR targeting ability was evaluated using cellular fluorescence assay in vitro. Three cell groups: experimental group(HT1080)、control group(HT29)、antagonistic group(HT1080)were prepared for the study. Fluorescent-NGR were dispensed with equivalent volume to experimental and control group at the same time, and Meanwhile NGR was dispensed to antagonistic group to seal CD13, then fluorescent-NGR was dispensed with equivalent volume as used in experimental group. The fluorescence intensity were compared among all three groups. Result Fluorescence intensity of the cells in experimental group were significantly higher than that in control group and antagonistic group according to a semi-quantitatively analysis(P 0.005). Fluorescence intensity of the cells of experimental group were significantly higher than that in HT29 of control group and HT1080 of antagonistic group(P 0.005). Conclusion Fluorescent-NGR-gadolinium can specifically target CD13 of HT1080 in vitro.
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| Chinese Journal Full-text Database |
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MENG Jie-Ru, YAN Zhen, ZHAO Ning, ZHANG Ying-QiBiotechnology Centre, Department of Pharmaceutics, Fourth Military Medical University, Xi’an 710033, China;High cell density fermentation of engneering E. coli expressing tumor targeted interferon-α2a[J];Journal of The Fourth Military Medical University;2004-23 |
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LIU Hai-feng, WANG Yu-rong~*, REN Yan(Department of Polymer Science and Materials , Dalian University of Technology,Dalian 116012, China);Development of Dendrimers' Practical Using Research[J];Polymer Bulletin;2005-03 |
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