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《Journal of Functional Polymers》 2001-01
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Study on the Synthesis of a Novel Packing Material —Butyl Cross-linked Chitosan for Hydrophobic Interaction Chromatography

WANG Zheng, GUO Min liang, JIANG Yong ming, WANG Yun, JIANG Shou lei, CHENG Yun (College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009,China)  
A novel hydrophobic interaction chromatographic(HIC) packing material has been synthesized by inducing butyl group to chitosan. In order to harden the HIC packing material, chitosan was cross linked by gluteraldehyde firstly. The butyl group was induced by the Schiff reaction of butyraldehyde to the free amino group of chitosan. The synthesizing conditions, particularly the concentrations of butyraldehyde and chitosan were investigated. The results showed that the concentrations of reactants affect the hydrophobic properties of the packing material significantly. In view of practice, the recommended concentrations of three reactants are 0.1% gluteraldehyde (V/V), 0.13%-0.26% butyraldehyde (V/V) and 2 g/L-10 g/L chitosan. However, the exact concentrations are dependent on the purified protein because different proteins have different hydrophobicity. Two kinds of chitosan HIC packing materials synthesized under different concentrations of butyraldehyde were used to purify crude ɑ-amylase (from Bacillus Subtilis). The results further demonstrated that the purification efficiency of the HIC packing material is dependent on their synthesizing conditions.
【Fund】: 江苏省教育厅资助!项目 ( 99KJB180 0 0 8)
【CateGory Index】: O631
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