Detecting Potato Spindle Tuber Viroid by Nucleic Acid Spot Hybridization
BAI Yan ju,LI Xue zhan,LU Dian qiu,HE Yun xia,ZHANG Ru xi,ZHU Cai(Biotechnology Research Center, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150086,China)
The specific probe of PSTVd was prepared by using fluorescein marker. Using nucleic acid spot hybridization method to detect PSTVd in field potato and at tube tissue. The result is empressed by explose. The result is clearly and repeatable. This method can detect PSTVd sensitively at least 0.33 pg.
【CateGory Index】： S435.32