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《Chinese Journal of Spectroscopy Laboratory》 2005-01
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Simultaneous Determination of Rutin and Quercertin in Fl os Sophorae Buds by Isoabsorptive Point Ultraviolet Spectrophotometry

LI Man-Xiu ZHANG Jing ZHANG Hai-Rong ZHAO Er-Lao(Department of Chemistry,Xinzhou Teacher's College,Xinzhou,Shanxi 03400 0,P.R.China)  
The article use isoabsorptive point ultraviolet spectrophotometry to determine rutin and quercertin in flos sophorae buds.Quercertin have the same absorption i n 247nm and 266nm,and rutin have the same absorption in 340nm and 370nm.The conc entration range of rutin and quercertin are from 4.32μg/mL to 48.65μg/mL.T he absorptions of the two substance at four wavelengths are all fitted by Beer' L aw.The method is easy,and it will be used in further research.
【CateGory Index】: O657.32
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