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《Chinese Journal of Spectroscopy Laboratory》 2009-01
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Study on Fingerprint Chromatograms of Sambucus Chinensis Lindl. by HPLC

ZOU Sheng-Qin a HUANG Hong-Wei b LIU Ming-Quan a CHEN Wu a,b a(Bioengineering Research Institute of Yichun University,Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines,Yichun,Jiangxi 336000,P.R.China) b(College of Chemistry and Bioengineering of Yichun University,Yichun,Jiangxi 336000,P.R.China)  
The fingerprint chromatograms of Sambucus chinensis Lindl. by HPLC was established,which can be applied for quality assessment and control of medicinal materials of Sambucus chinensis lindl.The HPLC analysis was performed on Kromasil C18 column (250mm×4.6mm,5μm) using ethanenitrile-0.2% aqueous solution of phosphoric acid (80:20) as mobile phase at flow rate of 0.8mL/min,and UV photodiode array detector at 215nm and the column temperature of 30℃.The fingerprint chromatograms of Sambucus chinensis lindl.,10 common peaks were found in the fingerprint chromatograms of Sambucus chinensis lindl. and good similarities with correlation coefficients over 0.9 were found in the fingerprint chromatograms between the herbs from different sources and standard fingerprint,which can be utilized for the identification of Sambucus chinensis lindl..The established HPLC fingerprint chromatograms have desirable accuracy,repeatability and stability,and can be used for one of the quality control of Sambucus chinensis lindl.,meanwhile,provide an experimental basis for further quality control of Sambucus chinensis lindl.
【Fund】: 江西省教育厅科技项目(赣教技字[2006]267号);; 国家“863”计划资助项目(2002AA2Z3217)
【CateGory Index】: R284
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