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《Chinese Journal of Spectroscopy Laboratory》 2010-01
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Extraction,Isolation and Determination of Polysaccharides from Cirsium Japonicum DC.

WU Yan WEI He-Ping(The Life Science Dept.of Anqing Normal College,Anqing,Anhui 246011,P.R.China)  
The crude polysaccharide was extracted from cirsium japonicum DC.using hot water-ethanol method to remove lipid and precipitate proteins.Its contents was determined by phenol-sulphuric acid method after the sample was freeze-dried.The yield of the crude polysaccharide was 6.5%.The sample was further isolated into five subfractions(DJ1,DJ2,DJ3,DJ4 and DJ5)through ion-exchange chromatography of DEAE-cellulose.The highest yield is 8.06‰ from DJ2.
【Fund】: 安徽省教育厅自然科学重点研究项目(KJ2007A123ZC);; 安徽省高等学校青年教师科研资助项目(2005jq1115)
【CateGory Index】: TQ461
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