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《Chinese Journal of Spectroscopy Laboratory》 2010-05
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Determination of Gypenoside A in Gynostemma Pentaphyllum (Thunb.) Makino by HPLC-ELSD

LE Yuana,c XIAO Ya-Pinga,b,c ZHANG Ninga,c HONG Ye-Lana,c XUE Jinga,ca(Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry,Xi'an 710062,P.R.China)b(National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China,Xi'an 710062,P.R.China)c(College of Life Science,Shaanxi Normal University,Xi'an 710062,P.R.China)  
Gypenoside A in Gynostemma pentaphyllum(Thunb.)Makino was determined by HPLC-ELSD.The separation was performed on Hibar C18 column(250×4.6mm,5μm),by gradient elution using acetonitrile-H2O as the mobile phase at the flow rate of 0.5mL/min,and the temperature of column was 20℃.The linear range of gypenoside A was 0.0425—1.995mg/mL(r2=0.9962).Also,the extraction was studied by the orthogonal experiment.This method was simple,effective and feasible,and can be used to control the quality of Gynostemma pentaphyllum(Thunb.)Makino.
【Fund】: “十一五”科技支撑计划项目(20060612)
【CateGory Index】: R284.1
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