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《Chinese Journal of Spectroscopy Laboratory》 2011-02
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Determination of Vitamin C in Bitter Gourd

SI Qin-Gerile EN De YI De-Ri WEI Shuang-Feng(College of Physics and Chemistry,Henan Polytechnic University,Jiaozuo ,Henan 454003,P.R.China)  
The amount of vitamin C in bitter gourd was determined directly by UV spectrophotometry with 10% hydrochloric acid as solvent.The absorbance of vitamin C shows a linear relationship with the concentration in the range of 0—10μg/mL with a regression equation of y=0.04746C+0.00109,r=0.9997.The detection limit is 0.023μg/mL,recovery is between 94.8%—101.1%.This method is simpler and less interference ion,accurate measurement of rapid,high accuracy and sensitive,and was applied to the determination of vitamin C.
【Fund】: 河南省科技计划重点攻关项目(092102210360);; 河南省煤矿安全项目(H09-43);; 河南省控制工程重点学科开放基金项目编号(KG2009-12);; 河南省教育厅自然科学研究计划项目(2009B480004);; 河南省“十一五”教育科学规划2008年课题项目(2008-JKGHAGH-295)
【CateGory Index】: O657.32;TS255.7
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