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《Chinese Journal of Spectroscopy Laboratory》 2011-06
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Determination of Plasma Concentration of Pyridostigmine Bromide in Oral Disintegrating Tablets by Liquid-liquid Extraction Reversed Phase Ion Pair Chromatography

ZHANG Li TAN Qun-Youa LIU Shan LUO Wen ZHANG Jing-Qing(Medicine Engineering Research Center,Chongqing Key Laboratory of Biochemical&Molecular Pharmacology,Chongqing Medical University,Chongqing 400016,P.R.China)a(Center of Thoracic Surgery,Daping Hospital & Research Institute of Surgery,Third Military Medical University,Chongqing 400042,P.R.China)  
The method for the determination of the plasma concentration of pyridostigmine bromide in oral disintegrating tablets was established by liquid-liquid extraction reversed phase ion pair chromatography.The plasma samples were treated by liquid-liquid extraction method by reversed phase ion pair separation method with pyridostigmine bromide as internal standard quantitative.The elutiontime of pyridostigmine bromide and internal standard substance were 5.63min and 9.76min,respectively,and that good separated with endogenous impurities in plasma.The linear range of pyridostigmine bromide was 0.02—2.00mg·L-1(n=7,r=0.9994),and absolute recoveries of samples were from 82.0%—89.2%,while relative recoveries were between 98.0% and 100.0%,and RSDs of inter-day and intra-day were 2.0%—4.1% and 3.1%—6.7%,respectively.When ratio of S and N was 3,the detection concentration of pyridostigmine bromide could be up to 0.005mg·L-1.The method is accurate and sensitive,and that can meet the need for the detection of plasma concentration and research on pharmacokinetic behavior.
【Fund】: 教育部博士点基金资助项目(20095503120008);; 重庆市教育委员会首批高等学校优秀人才资助(KJ090308)
【CateGory Index】: R96
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 FU Wen-huan,SHI Xiao-jin,LI Zhong-dong,ZHONG Ming-kang,XU Lu-yang(Department of Clinical Pharmacology,Huashan Hospital Affiliated to Fudan University,Shanghai 200040,China);Determination of Pyridostigmine Bromide in Human Plasma by RP-HPLC[J];Chinese Pharmaceutical Journal;2007-12
2 ZHANG Da-fu(Department of Pharmacy,Yuehua Hospital of Yueyang,Yueyang Hunan 414000);Determination of pyridostigmine bromide in human plasma by reversed phase ion pair chromatography and its application in bioequivalence[J];Central South Pharmacy;2009-04
3 WANG Li-jun1,WANG Dong-kai1,LI Ling1,DENG Shuo1,YANG Xiu-li1,KONG Li-wen1,YIN Dong-er2(1 Department of Pharmaceutics,Shenyang Pharmaceutical University,Shenyang 110016,China;2 Hainan Bikai Pharmaceutical Co.,Ltd.,Haikou 570216,China);Preparation and assay of pyridostigmine bromide injection[J];Chinese Journal of New Drugs;2007-11
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 WANG Hong1,TAN Qun-you2,ZHANG Li1,CHENG Xun-guan3,ZHANG Jing-qing1 1Medicine Engineering Research Center in University,3Research Lab of Medicinal Chemistry and Biological Material,Chongqing Key Laboratory of Biochemical&Molecular Pharmacology,Chongqing Medical University,Chongqing 400016,China;2Department of Thoracic Surgery,Daping Hospital & Research Institute of Surgery,Third Military Medical University,Chongqing 400042,China;Bioequivalence of pyridostigmine bromide dispersible tablets in rabbits[J];Journal of Southern Medical University;2011-10
2 LI Yi TAN Qun-Youa JIANG Xin-Hui ZHAO Chun-Jingb ZHANG Jing-Qing(Medicine Engineering Research Center,Chongqing Key Laboratory of Biochemical & Molecular Pharmacology,Chongqing Medical University,Chongqing 400016,P.R.China)a(Center of Thoracic Surgery,Daping Hospital & Research Institute of Surgery,Third Military Medical University,Chongqing 400042,P.R.China)b(Pharmacy Department,The Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,P.R.China);Determination of Pyridostigmine Bromide in Bovine Serum Ablum Microspheres by HPLC[J];Chinese Journal of Spectroscopy Laboratory;2011-05
3 FAN Jun1,2,HE Wen1(1.Department of Pharmacy,Renmin Hospital of Wuhan University,Wuhan 430060,China;2.College of Pharmacy,Wuhan University,Wuhan 430072,China);Study on the preparation and in vitro release of pyridostigmine bromide liposomes coated by N-trimethyl chitosan[J];Journal of Guangdong Pharmaceutical University;2011-05
4 ZHANG Li1,TAN Qun-you2,CHENG Xun-guan3,ZHANG Chun1,XU Mei-ling1,ZHANG Jing-qing1(1.Medicine Engineering Research Center in University,Chongqing Key Laboratory of Biochemical&Molecular Pharmacology,Chongqing Medical University,Chongqing 400016,China;2.Daping Hospital & Research Institute of Surgery,Chongqing 400042,China;3.Research Lab of Medicinal Chemistry and Biological Material,Chongqing Medical University,Chongqing 400016,China);Study on pharmacokinetics of pyridostigmine bromide orally disintegrating tablets and common tablets in rabbits[J];Chinese Journal of Hospital Pharmacy;2011-01
5 ZHANG Da-fu(Department of Pharmacy,Yuehua Hospital of Yueyang,Yueyang Hunan 414000);Determination of pyridostigmine bromide in human plasma by reversed phase ion pair chromatography and its application in bioequivalence[J];Central South Pharmacy;2009-04
6 CAI Zi-you,LI Yong-chong(Guangdong Food and Drug Vocational College,Guangzhou 510520,China) CHEN Zuan-guang(School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China);Determination of Pyridostigmine Bromide in Pyridostigmine Bromide Tablets by Microfluidic Chip with Contactless Conductivity Detection[J];China Pharmacy;2011-28
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 FU Wen-huan,SHI Xiao-jin,LI Zhong-dong,ZHONG Ming-kang,XU Lu-yang(Department of Clinical Pharmacology,Huashan Hospital Affiliated to Fudan University,Shanghai 200040,China);Determination of Pyridostigmine Bromide in Human Plasma by RP-HPLC[J];Chinese Pharmaceutical Journal;2007-12
2 LIU Zhi-wu(First People's Hospital of Yueyang,Hunan 414000);Determination of residual solvents in pyridostigmine bromide by headspace gas chromatography[J];Central South Pharmacy;2007-04
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