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《Chinese Journal of Spectroscopy Laboratory》 2011-06
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Effects of Different Extraction Methods on Extracting Ratios of Total Alkaloids of Oxytropis Glabra DC

WANG Shuai CHEN Gen-Yuan WU Shu-Qi HU Jian-Jun MA Chun-Hui(Key Laboratory of Tarim Animal Husbandry Science and Technology,Xinjiang Production & Construction Corps,College of Animal Science,Tarim University,Alar,Xinjiang 843300,P.R.China)  
Total alkaloids of Oxytropis Glabra DC were extracted by 3 methods,including ultrasonic wave assisting extraction,ethanol digestion-organic solvent extraction process and ethanol digestion-column chromatography separation.The extracting ratios of total alkaloids for the methods were compared.The extraction ratios were 0.0008%,0.0032% and 0.0080%.The third extraction is best.The optimum conditions were extraction temperature of 30℃,ethanol concentration of 75% and the ratio of mass to volume of 1∶15(g/mL) through orthogonal tests.The extracting ratio of total alkaloids of Oxytropis Glabra DC was high by ethanol digestion-column chromatography separation with ethanol concentration of 75% as solution.
【Fund】: 新疆生产建设兵团博士基金项目(08BB08);; 新疆兵团塔里木畜牧科技重点实验室开放课题(HS20904)
【CateGory Index】: TQ464.4
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