Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Spectroscopy Laboratory》 2012-02
Add to Favorite Get Latest Update

Inhibition Effect of Radix Polygonum Multiflorum Thumb Extractive on Acetylcholinesterase

SHUAI Li YANG Tian-Ming CHEN Jing-Si YAN Shuai-Yonga(College of Pharmacy,Central South University for Nationalities,Wuhan 430074,P.R.China)a(School of Economics,Huazhong University of Science & Technology,Wuhan 430074,P.R.China)  
The screening of inhibitory activity and kinetics of radix polygonum multiflorum thumb on acetylcholinesterase(AChE) were investigated,and inhibitory activity of radix polygonum multiflorum thumb ethylacetate fractions and water fractions were gradually weakened as the inhibitor concentration increased,and even showed some enhancement,water fractions didn't have any inhibitory activity of AChE.Water-saturated n-butyl alcohol fractions and petroleum ether fractions components were significant inhibition activity for AChE.Reversible inhibition of AChE by both of them was observed,and the types of reversible inhibition were non-competitive reversible inhibition and mixed inhibition,respectively.The non-competitive reversible inhibitory constants Ki and Kis were 3.2668μmol/L and 4.8194μmol/L for water-saturated n-butyl alcohol fraction components.The mixed inhibitory constants Ki and Kis were 2.6985μmol/L and 4.7192μmol/L for petroleum ether fraction components,respectively.The inhibitory kinetic results indicated that the inhibition effects of fraction components on free enzyme were stronger than those on enzyme-substrate complex,and the inhibition effects of petroleum ether fraction components were stronger than those of water-saturated n-butyl alcohol fraction components.
【Fund】: 中南民族大学自然科学青年启动基金(YZQ10008)
【CateGory Index】: R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 5 Hits
1 HOU De-ren, YANG Qi-dong, ZHOU Lin, et al. Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China;Effect of Polygonum Multiflorum Thunb on Learning-memory Ability in Rats with Alzheimer Disease and Its Probable Mechanism[J];Journal of Chinese Physician;2004-03
2 ZHANG Yi, FENG Yan, LI Xiao-Ming , WANG Bin-Gui(Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071; Graduate School, Chinese Academy of Sciences, Beijing, 100039) (Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071);ACETYLCHOLINESTERASE INHIBITORY SCREENING FOR FRACTIONS OF SEAWEEDS[J];Oceanologia Et Limnologia Sinica;2005-05
3 LIU Qiongyu1,2,ZHOU Peijiang1,CHEN Yan1(1.College of Resources and Environmental Sciences,Wuhan University/Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory,Wuhan 430079,Hubei,China;2.School of Chemistry and Environmental Engineering,Jianghan University,Wuhan 430056,Hubei,China);Inhibition Effect of Chlorophenols on Acetylcholinesterase and Its Kinetics[J];Journal of Wuhan University(Natural Science Edition);2010-05
4 CHENG Xi1 CHEN Jun1 ZHU Da-Yuan2(1.College of Pharmacy,Jiangsu University,Zhenjiang 212013)(2.State Key Laboratory of Drug Research,Shanghai Institute of Medicines,Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences,Shanghai 201023);Initially Research in Acetylcholinesterase Inhibitory Activity of Endophytic Fungi g5 Isolated from Huperzia serrata[J];Microbiology;2008-11
5 WANG Wei and WANG Dan-qiao Institute of Geriatrics, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing (100091);Progress of Study on Brain Protective Effect and Mechanism of Polygonum multiflorum[J];Chinese Journal of Integrated Traditional and Western Medicine;2005-10
Chinese Journal Full-text Database 10 Hits
1 WANG Yan et al (Institute for Agricultural Application of Atomic Energy,AAAS,Hefei,Anhui 230031);Effect of Rare Earth Element La on Enzyme Activity and N Absorbtion of Wheat[J];Journal of Anhui Agricultural Sciences;2006-15
2 LI Yuan-yuan et al (Academy of Biology and Geography of Qinghai Normal University,Xining,Qinghai 810008);Research of Antioxidants and Lipid Peroxidation from Pea Crops of Plateau[J];Journal of Anhui Agricultural Sciences;2009-20
3 LIU Zhen et al(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan 610031);Research on the Antioxidase Activities of Tetraploid Lines and Diploid Control Line of Chrysanthemum cinerariifolium[J];Journal of Anhui Agricultural Sciences;2010-05
4 ZHAO Jiang-sha,ZENG Zhao,XIONG Yuan,XIE Zhi-xiong ,GUO Yi-qing,SHAO Xue-ling ( College of Life Science, Wuhan University, Wuhan 430072, Hubei, China);Influence of Cu~(2+) on the Activity of Dugesia japonia Catalase[J];Amino Acids & Biotic Resources;2003-03
5 YU Cheng-fan,HUANG Xiao-bo,CHEN Liang,XU Qing-quan,HU Wei-guo,WANG Xiao-feng(Department of Urology,Peking University People's Hospital,Beijing 100034,China);Effect of nanobacteria on cell damage and crystal retention in renal tubular epithelial cells[J];Journal of Peking University(Health Sciences);2010-04
6 LIU Cun-qi,WANG Wei-wei,ZHANG Ya-juan(Department of Life Science,Hebei University,Baoding 071002,China);Advance on Superoxide Dismutase in Aquatic Organisms[J];Fisheries Science;2005-11
7 WANG Fan(Life Science Department,Luoyang Normal University,Luoyang 471022,China);Progress of Research on Molecular Biomarkers for Monitoring Pesticide Contamination in Aquatic Animals[J];Fisheries Science;2007-11
8 WANG Wei-wei1,LIU Cun-qi2,LI Dao-ji1,ZHANG Ya-juan2(1.State Key Laboratory of Estuarine and Coastal Research,East China Normal University,Shanghai 200062,China;2.College of Life Science,Hebei University,Baoding 071002,China);Partial Characterizations of Superoxide Dismutase in Three Species of Shrimps[J];Fisheries Science;2009-04
9 TONG Guo-lin, ZHANG Xiao-li, JING Yi (Nanjing Forestry University, Nanjing 210037,Jiangsu, China);A study on improving the surface sizing performance of starch treated with amylase enzymolysis[J];China Pulp & Paper Industry;2007-12
10 LIU Bei WANG Hai-yi (Shaanxi University of Science and Technology,Xi'an,Shaanxi,China);A preliminary study on using electro-phoresis to identify catalase in DIP systems[J];China Pulp & Paper Industry;2008-06
China Proceedings of conference Full-text Database 4 Hits
1 WANG Gui-ling, XU Hang, CHEN Zhi-guo, SHEN Wei-rong College of Aqua - life Science and Technology, Shanhai Fisheries University, Shanghai, ,200090 School of life science, Ningxia University, Yinchuan 750021;Studies on four isozyme systems in sex reversal process of Monopterus albus[A];[C];2005
2 ZHANG Hong-wei, WANG Feng-shan*(Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Science, Shandong University, Jinan 250012, China);Secondary structure analysis of Cu,Zn-Superoxide dismutase modified by low molecular weight heparin[A];[C];2006
3 ZHAO Li1,2,XU Shu-Ping1,ZHANG Lei2,SONG Bo1,LI Zong-yang1,PAN Rui-le1 (1. Institute of Medicinal Plant,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;2. Tianjin University of Science and Technology,Tianjin 300457,China);Studies on the antioxidant and acetylcholinesterase activities of Myricitrin and its structure-similar compounds[A];[C];2010
4 Zhang Yong-jun1,Xian Qiao2,Zhao Meng-yao3,He Jie-min4 1) College of Life Sciences,China Jiliang University,Hangzhou,China 2) College of Life Sciences,China Jiliang University,Hangzhou,China 3) College of Life Sciences,China Jiliang University,Hangzhou,China 4) College of Life Sciences,China Jiliang University,Hangzhou,China;Study on active center of a chitinase BSCH0802 with the cell walls disrupting of Auricularia auricula[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Gong Renmin (Department of Biology, Anhui Normal University, 241000, Wuhu, Anhui, PRC);THE LINEAR MIXED INHIBITION KINETICS AMONG REVERISIBLE INHIBITION TO ENZYME[J];Journal of Anhui Normal University(Natural Science);1998-01
2 LIU Bin~(1), ZHOU Pei-jiang~(1), WU Xin-guo~(1), SONG Li-rong~(2) (1. School of Resource and Environment Science, Wuhan University, Wuhan 430072; 2.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072);Inhibition Effect of Benzidine on Alkaline Phosphatase and Its Kinetics Study[J];Environmental Science and Technology;2005-03
4 WANG Jing-yun 1,PENG Xiao-jun 1,YANG Dong 2,AN Li-jia 2,HU Jie-han 3,and ZHENG Xue-fang 2 1 Dept of Bioengineering,Dalian University of Technology,Dalian 116012,China 2 State Key Laboratory of Fine Chemicals,Dalian University of Technology,Da;Kinetics of Inactivation of Calf Intestine Alkaline Phosphatase by EDTA with Absorption Spectrum Method[J];Spectroscopy and Spectral Analysis;2001-05
5 SONG Shi jun 1,LI Fang fang 2* ,YUE Hua 1,YIN Zhi wei 2 (1.Department of Physiology,the School of Basic Medical Sciences,Hebei Medical University, Shijiazhuang 050017, China;2.Department of Biochemistry, the School of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 050017, China);STUDY ON THE ANTI-AGING EFFECTS OF RADIX POLYGONUM MULTIFLORUM[J];Journal of Hebei Medical University;2003-02
6 LIU Zheng-tao,LI Zhao-li,LI ZhengSEPA Key Laboratory of Ecological Effect and Risk Assessment of Chemicals,Chinese Research Academy of Environmental Sciences,Beijing 100012,China;Joint Toxicity of Chlorophenols to Freshwater Photobacterium Vibrio Qinghaiensis sp.-Q67[J];Research of Environmental Sciences;2008-02
7 Han Daxiong, Yang Pin+*(Institute of Molecular Science, Shanxi University, Taiyuan 030006,China);Multiple Molecular Etiologies of Alzheimer Disease[J];Chemistry;2003-02
8 XU Nian-Jun, FAN Xiao, HAN Li-Jun, YAN Xiao-Jun, ZENG Cheng-Kui(C.K.Tseng) (Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071);SCREENING MARINE ALGAE FROM SHANDONG COAST FORANTITUMOR ACTIVITY[J];Oceanologia Et Limnologia Sinica;2001-04
9 NIU Rong Li, FAN Xiao, HAN Li Jun (Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071);A SCREENING FOR THE ANTI-INFLAMMATORY EFFECT OF ALGAL EXTRACTS[J];Oceanologia Et Limnologia Sinica;2003-02
10 XU Xiu Li, FAN Xiao  , SONG Fu Hang (Institute of Oceanology, Chinese Academy of Sciences,Qingdao, 266071; Graduate school of the Chinese Academy of Sciences,Beijing, 100039)  (Institute of Oceanology, Chinese Academy of Sciences,Qingdao,266071);STUDY ON BIOACTIVITY OF CHINESE MARINE ALGA EXTRACTS[J];Oceanologia Et Limnologia Sinica;2004-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved