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《Chinese Journal of Spectroscopy Laboratory》 2012-02
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Inhibition Effect of Radix Polygonum Multiflorum Thumb Extractive on Acetylcholinesterase

SHUAI Li YANG Tian-Ming CHEN Jing-Si YAN Shuai-Yonga(College of Pharmacy,Central South University for Nationalities,Wuhan 430074,P.R.China)a(School of Economics,Huazhong University of Science & Technology,Wuhan 430074,P.R.China)  
The screening of inhibitory activity and kinetics of radix polygonum multiflorum thumb on acetylcholinesterase(AChE) were investigated,and inhibitory activity of radix polygonum multiflorum thumb ethylacetate fractions and water fractions were gradually weakened as the inhibitor concentration increased,and even showed some enhancement,water fractions didn't have any inhibitory activity of AChE.Water-saturated n-butyl alcohol fractions and petroleum ether fractions components were significant inhibition activity for AChE.Reversible inhibition of AChE by both of them was observed,and the types of reversible inhibition were non-competitive reversible inhibition and mixed inhibition,respectively.The non-competitive reversible inhibitory constants Ki and Kis were 3.2668μmol/L and 4.8194μmol/L for water-saturated n-butyl alcohol fraction components.The mixed inhibitory constants Ki and Kis were 2.6985μmol/L and 4.7192μmol/L for petroleum ether fraction components,respectively.The inhibitory kinetic results indicated that the inhibition effects of fraction components on free enzyme were stronger than those on enzyme-substrate complex,and the inhibition effects of petroleum ether fraction components were stronger than those of water-saturated n-butyl alcohol fraction components.
【Fund】: 中南民族大学自然科学青年启动基金(YZQ10008)
【CateGory Index】: R285.5
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