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《Chinese Journal of Spectroscopy Laboratory》 2012-03
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Qualitative and Quantitative Analysis of Alpha-Eleostearic Acid in G.Pentaphyllum Seeds

JIANG Dong-Liang MA Cai-Xia XIAO Ya-Ping(College of Life Sciences,Shaanxi Normal University,Xi'an 710062,P.R.China)  
A gas chromatography-mass spectrometry(GC-MS) was established to identify composition and determine the content of alpha-eleostearic acid in G.Pentaphyllum seeds,with RTX-5MS fused-silica capillary column(0.25mm×30m×0.25μm).The composition of alpha-eleostearic acid was qualitatively identified through the relative retention time and mass spectrometry,alpha-eleostearic acid was quantitatively determined by internal standard method.The results revealed that alpha-eleostearic acid was detected and the content was high.The determination of alpha-eleostearic acid using GC-MS was expressed to be A=2.7924C-0.4851 with a correlation coefficient of 0.9996.An excellent linear range for alpha-eleostearic acid was 0.34—1.37mg/mL.The average recovery was 99.91%.Total content of alpha-eleostearic acid in G.pentaphyllum seeds was 66.9mg/g.The high yield suggests an effective way for sustainable production of alpha-eleostearic acid.
【Fund】: 国家“十五”科技攻关项目(2001BA701A35)
【CateGory Index】: R284
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 QI Gang,ZHANG Li;Progress of Gynostemma on pharmacology[J];Acta Academiae Medicinae Cpapf;2003-03
2 Liu Guoqiao. Guo Duanlai, Zhong Ronghui (Guangzhou Pharmaceutical Industrial Research Institute, Guangzhou 510240) Hu Wenmei (Guangdong Pharmaceufical School, Guangzhou 510520);Isolation and Identification of the 2α-hydroxypanaxadiol Saponins in Gynostemma pentaphyllum[J];Journal of Chinese Medicinal Materials;1996-10
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LE Yuana,c XIAO Ya-Pinga,b,c ZHANG Ninga,c HONG Ye-Lana,c XUE Jinga,ca(Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry,Xi'an 710062,P.R.China)b(National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China,Xi'an 710062,P.R.China)c(College of Life Science,Shaanxi Normal University,Xi'an 710062,P.R.China);Determination of Gypenoside A in Gynostemma Pentaphyllum (Thunb.) Makino by HPLC-ELSD[J];Chinese Journal of Spectroscopy Laboratory;2010-05
2 NI Hong,LI Wei,XIONG Zhe,ZENG Si-quan(College of Life Science,Hubei University,Wuhan 430062,China);Advances of Pharmacological and Clinical Research in Gypenosides[J];Journal of Hebei Agricultural Sciences;2011-09
3 SHEN Hong-chun (Bioengineering Department of Enshi Technical College,Enshi 445000,China);Effects of Gyrtostemma on Antioxidant Capacity and Immune Functions in Weaning Piglets[J];Journal of Hubei University for Nationalities(Natural Science Edition);2012-01
4 PENG Xiao-lie1,WANG Sha-sha1,LIU Shi-biao1,PENG Xiu-jian2,ZHANG Xian-zhuo2 (1.College of Biology and Environmental Science,Jishou University,Hunan 416000,PRC; 2.Hunan Xiangquan Pharmaceutical Company Limited,Jishou 416000,PRC);Effects of Temperature and Plant Growth Regulator on Seed Germination of Gynostemma pentaphyllum[J];Hunan Agricultural Sciences;2011-19
5 ZHONG Hui (Xiaogan Third Hospiatl,Xiaogan 432100,Hubei);Studies on the Fingerprint Spectrum of Flavonoids in Gynostemma pentaphyllum[J];Journal of Hubei University of Chinese Medicine;2010-03
6 LOU Jia-ning,GE Shu-yun,ZHOU Zeng-tong.1.Department of Stomatology,First People's Hospital,Shanghai Jiaotong University;Shanghai 200080,China;2.Department of Oral Medicine,College of Stomatology,Ninth People's Hospital,School of Medicine,Shanghai Jiaotong University;Research Institute of Stomatology;Shanghai Key Laboratory of Stomatology,Shanghai 200011,China.;The study of altered succinate dehydrogenase gene expression by Gypenosides during rat tongue OLK carcinogenesis induced by 4-Nitroquinoline 1-Oxide.[J];Journal of Clinical Stomatology;2010-12
7 ZHANG Ning1,2,LIN Hong1,SUI Tao2,ZHONG Xiang2,ZHANG Yi-Bing3,LIU Feng4(1.Seafood Safety Laboratory,Ocean University of China,Qingdao 266003,China;2.Yantai Inspection and Quarantine Bureau,Yantai 264000,China;3.Shangdong Entry-Exit Inspection and Quarantine Bureau,Qingdao 266001,China;4.China Agricultural University(Yantai),Yantai 264670,China);Research on Fat-Soluble Toxicant in Stored Frozen Oyster[J];Periodical of Ocean University of China;2010-01
8 YUE Lin-na1,GAO Xue-ling1,YUE Peng-xiang2 (1 School of Tea and Food Science,Anhui Agriculture University,Hefei 230036,Anhui,China; 2 Damin Foodstuff(Zhangzhou)Co.,Ltd.,Zhangzhou 363000,Fujian,China);Advances in studies of effective components in Gynostemma pentaphyllum[J];The Beverage Industry;2008-06
9 LIU Gong-rang1,GUAN Pei-zhong,SONG Shu-liang,LU Su-mei,FENG Yu-xin,XIN Hua(1 Medicial College of Shandong University,Jinan 250012,P.R.China);Protective effect of gynostemma pentaphyllum makino polysaccharide on HepG2 cells injured by CCl_4[J];Shandong Medical Journal;2007-31
10 ZHOU Liang,LIU Yuan-fa,JIN Qing-zhe,WANG Xing-guo (School of Food Science and Technology, Jiangnan University, Key Laboratory of Food Science and Security, Ministry of Education, Wuxi 214122, China);Analysis on Physic-chemical Properties of Trichosanthes kirilowii Seed Oil and Structure of Trichosanic Acid[J];Food Science;2007-11
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Yan Junfeng; Li Jin rong; et al.(Stomatological College of Hu Bei Medical University, Wu Han, 430070.);The Suppressive Effect of Gypenosides On Neck Lymph Node Metastasis Cell Line Derived FromHuman Oral Squamous Cell Carcinoma In vitro[J];JOURNAL OF CLINICAL STOMATOLOGY;1998-03
2 Zhang Fang, Li Shentian (The Affiliated Hospital of Shandong Medical University);PROTECTIVE EFFECT OF GYPENOSIDES ON LEFT VENTRICULAR CONSTRICTION IN RATS WITH EXPERIMENTAL MYOCARDIAL ISCHEMIA[J];ACTA ACADEMIAE MEDICINAE SHANDONG;1999-01
3 ZHAO Ying, HU DaYi(Lab of Heart Center, Beijing Red Cross Chao Yang HospitalAffiliate of Capital University of Medicine, Beijing 100020);Effect of GP on action potentials,Lcalcium channel currents, sodium channel currents and potassium channel currents in isolated single ventricular myocyte of guinea pigs[J];CHINESE PHARMACOLOGICAL BULLETIN;1998-01
4 HUO HaiRu, WANG TianYou1, YU ShuRen, XIAO PeiGen(Institute of Medicinal Plant Development, Peking Union Medical Collegeand Chinese Academy of Medical Sciences, Beijing 100094);Protective effect of saponins of gynostemma pubescens on neuronal injury in cultured cortical neurons of mice[J];CHINESE PHARMACOLOGICAL BULLETIN;1998-02
5 Chen Jianxiong;Liao Duan fang;Tang Xiaoqing;et al (Address:Chen Jianxiong,Department of Pharmacology,Hengyang Medical College,Hengyang);Protection of Gypenoside Against Oxygen Free Radical Induced Vasospasm of Isolated Rabbit Basilar Artery[J];CHINESE TRADITIONAL AND HERBAL DRUGS;1997-04
6 QI Gang, ZHANG Li, XIE Wen-Li, CHEN Xiao-Yi, LI Ji-Sheng(Department of Pharmacology, Medical College of CPAPF, Tianjin 300162, China);Protective effect of gypenosides on DNA and RNA of rat neurons in cerebral ischemia-reperfusion injury[J];中国药理学报(英文版);2000-12
7 Qian Xinghua, Wang Yixian, Tang Xiaoling 1, Zhang Qinfang, Gao Ling, Kang Shouhai Jiangsu Institute of Cancer Research, Nanjing 210009; 1Jiangsu Provincial Peoples Hospital 1, Nanjing 210029;Effects of Gynostemma pentaphyllum Makino Polysaccharide (PGP) on Immune Function[J];JOURNAL OF CHINA PHARMACEUTICAL UNIVERSITY;1999-01
8 Li Qianning (Li QN), Wang Dongwu (Wang DW), Yue Guiming (Yue GM), et al (Department of Neuropathology, Xinqiao Hospital, Third Millitary Medical College, Chongqing 630037);The protective effect of gypenoside on brainstem ischemia and its mechanism[J];CHINESE PHARMACEUTICAL JOURNAL;1997-08
9 Sun Wansen,Qiao Chenglin,Cheng Wei,et al.The Second Clinical Medical College of Xian Medical University ,Xian 710004;Effect of gypenoside on plasma endothelin and renal function in rats with chronic renal insufficiency[J];CHINESE CRITICAL CARE MEDICINE;1998-05
10 Wang Bin,Ge Zhidong,Zhou Aiwu,et al. Clinical Pharmacology Institute,Anhui Medical University,Hefei 230032;Effects of Gypenosides on Immune Function of Rats in Vitro[J];TRADITIONAL CHINESE DRUG RESEARCH & CLINICAL PHARMACOLOGY;1999-01
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