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《Chinese Journal of Spectroscopy Laboratory》 2012-03
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Qualitative and Quantitative Analysis of Alpha-Eleostearic Acid in G.Pentaphyllum Seeds

JIANG Dong-Liang MA Cai-Xia XIAO Ya-Ping(College of Life Sciences,Shaanxi Normal University,Xi'an 710062,P.R.China)  
A gas chromatography-mass spectrometry(GC-MS) was established to identify composition and determine the content of alpha-eleostearic acid in G.Pentaphyllum seeds,with RTX-5MS fused-silica capillary column(0.25mm×30m×0.25μm).The composition of alpha-eleostearic acid was qualitatively identified through the relative retention time and mass spectrometry,alpha-eleostearic acid was quantitatively determined by internal standard method.The results revealed that alpha-eleostearic acid was detected and the content was high.The determination of alpha-eleostearic acid using GC-MS was expressed to be A=2.7924C-0.4851 with a correlation coefficient of 0.9996.An excellent linear range for alpha-eleostearic acid was 0.34—1.37mg/mL.The average recovery was 99.91%.Total content of alpha-eleostearic acid in G.pentaphyllum seeds was 66.9mg/g.The high yield suggests an effective way for sustainable production of alpha-eleostearic acid.
【Fund】: 国家“十五”科技攻关项目(2001BA701A35)
【CateGory Index】: R284
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