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《Chinese Journal of Spectroscopy Laboratory》 2012-06
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Chemical Compositions and Antioxidant Activity of Essential Oil from Seeds of Notopterygium incisum Ting ex H.T.Chang

LIU Wei-Gena,b ZHOU Guo-Yinga XU Wen-Huaa,b LI Chun-Lia,b YANG Lu-Guna SUN Jingaa(Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,P.R.China)b(Graduate University of Chinese Academy of Sciences,Beijing 100049,P.R.China)  
The essential oil from seeds of Notopterygium incisum Ting ex H.T.Chang was extracted by steam distillation(SD),Soxhlet extraction(SE) and supercritical CO2 extraction(SFE-CO2),and qualitatively and quantitatively analyzed by GC-MS.The antioxidant activities of the oil extracted by SFE-CO2 were assessed by 2,2′-diphenyl-1-picrylhydrazyl radical scavenging method(DPPH) and ferric reducing/antioxidant power assay(FRAP),and the typical antioxidant butylated hydroxytoluene(BHT) was used as control.The results showed that the appearance,yield,chemical components and contents of the extracted essential oil by the three methods were different from each other,and the yields of the extracted oil by SD,SE and SFE-CO2 were respectively 2.3%,12.9%,7.6%;and 35,50,55 kind of components were respectively identified,representing 94.64%,99.93%,99.98% of the total essential oil,respectively.The extracted essential oil by SFE-CO2 scavenged DPPH radical with IC50 1.9mg/mL,and the FRAP value increased along with increasing the concentration with linear regression equation y=0.1958x-0.0128(R2=0.9973).The essential oil of seeds of Notopterygium incisum Ting ex H.T.Chang has some degree of antioxidant capacity,and shows the obvious dose-response relationship,but the relationship was weaker than that of synthetic antioxygen BHT.The extracted essential oil by SFE-CO2 can really,comprehensively reflect the chemical compositions of medicinal materials.
【Fund】: 中国科学院“西部之光”人才培养计划项目(2007年);; 科技部科技人员服务企业行动项目(2009GJG20016)
【CateGory Index】: R284
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